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Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging

Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that are secreted by cells and facilitate cell-to-cell communication by their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over the last decade, from disease...

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Autores principales: Gupta, Dhanu, Liang, Xiuming, Pavlova, Svetlana, Wiklander, Oscar P.B, Corso, Giulia, Zhao, Ying, Saher, Osama, Bost, Jeremy, Zickler, Antje M., Piffko, Andras, Maire, Cecile L., Ricklefs, Franz L., Gustafsson, Oskar, Llorente, Virginia Castilla, Gustafsson, Manuela O., Bostancioglu, R. Beklem, Mamand, Doste R, Hagey, Daniel W., Görgens, André, Nordin, Joel Z., EL Andaloussi, Samir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481830/
https://www.ncbi.nlm.nih.gov/pubmed/32944187
http://dx.doi.org/10.1080/20013078.2020.1800222
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author Gupta, Dhanu
Liang, Xiuming
Pavlova, Svetlana
Wiklander, Oscar P.B
Corso, Giulia
Zhao, Ying
Saher, Osama
Bost, Jeremy
Zickler, Antje M.
Piffko, Andras
Maire, Cecile L.
Ricklefs, Franz L.
Gustafsson, Oskar
Llorente, Virginia Castilla
Gustafsson, Manuela O.
Bostancioglu, R. Beklem
Mamand, Doste R
Hagey, Daniel W.
Görgens, André
Nordin, Joel Z.
EL Andaloussi, Samir
author_facet Gupta, Dhanu
Liang, Xiuming
Pavlova, Svetlana
Wiklander, Oscar P.B
Corso, Giulia
Zhao, Ying
Saher, Osama
Bost, Jeremy
Zickler, Antje M.
Piffko, Andras
Maire, Cecile L.
Ricklefs, Franz L.
Gustafsson, Oskar
Llorente, Virginia Castilla
Gustafsson, Manuela O.
Bostancioglu, R. Beklem
Mamand, Doste R
Hagey, Daniel W.
Görgens, André
Nordin, Joel Z.
EL Andaloussi, Samir
author_sort Gupta, Dhanu
collection PubMed
description Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that are secreted by cells and facilitate cell-to-cell communication by their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over the last decade, from disease pathophysiology to therapeutic drug delivery, improved molecular tools to track their therapeutic delivery are still needed. Unfortunately, the present catalogue of tools utilised for EV labelling lacks sensitivity or are not sufficiently specific. Here, we have explored the bioluminescent labelling of EVs using different luciferase enzymes tethered to CD63 to achieve a highly sensitive system for in vitro and in vivo tracking of EVs. Using tetraspanin fusions to either NanoLuc or ThermoLuc permits performing highly sensitive in vivo quantification of EVs or real-time imaging, respectively, at low cost and in a semi-high throughput manner. We find that the in vivo distribution pattern of EVs is determined by the route of injection, but that different EV subpopulations display differences in biodistribution patterns. By applying this technology for real-time non-invasive in vivo imaging of EVs, we show that their distribution to different internal organs occurs just minutes after administration.
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spelling pubmed-74818302020-09-16 Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging Gupta, Dhanu Liang, Xiuming Pavlova, Svetlana Wiklander, Oscar P.B Corso, Giulia Zhao, Ying Saher, Osama Bost, Jeremy Zickler, Antje M. Piffko, Andras Maire, Cecile L. Ricklefs, Franz L. Gustafsson, Oskar Llorente, Virginia Castilla Gustafsson, Manuela O. Bostancioglu, R. Beklem Mamand, Doste R Hagey, Daniel W. Görgens, André Nordin, Joel Z. EL Andaloussi, Samir J Extracell Vesicles Research Article Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that are secreted by cells and facilitate cell-to-cell communication by their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over the last decade, from disease pathophysiology to therapeutic drug delivery, improved molecular tools to track their therapeutic delivery are still needed. Unfortunately, the present catalogue of tools utilised for EV labelling lacks sensitivity or are not sufficiently specific. Here, we have explored the bioluminescent labelling of EVs using different luciferase enzymes tethered to CD63 to achieve a highly sensitive system for in vitro and in vivo tracking of EVs. Using tetraspanin fusions to either NanoLuc or ThermoLuc permits performing highly sensitive in vivo quantification of EVs or real-time imaging, respectively, at low cost and in a semi-high throughput manner. We find that the in vivo distribution pattern of EVs is determined by the route of injection, but that different EV subpopulations display differences in biodistribution patterns. By applying this technology for real-time non-invasive in vivo imaging of EVs, we show that their distribution to different internal organs occurs just minutes after administration. Taylor & Francis 2020-08-21 /pmc/articles/PMC7481830/ /pubmed/32944187 http://dx.doi.org/10.1080/20013078.2020.1800222 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gupta, Dhanu
Liang, Xiuming
Pavlova, Svetlana
Wiklander, Oscar P.B
Corso, Giulia
Zhao, Ying
Saher, Osama
Bost, Jeremy
Zickler, Antje M.
Piffko, Andras
Maire, Cecile L.
Ricklefs, Franz L.
Gustafsson, Oskar
Llorente, Virginia Castilla
Gustafsson, Manuela O.
Bostancioglu, R. Beklem
Mamand, Doste R
Hagey, Daniel W.
Görgens, André
Nordin, Joel Z.
EL Andaloussi, Samir
Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
title Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
title_full Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
title_fullStr Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
title_full_unstemmed Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
title_short Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
title_sort quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481830/
https://www.ncbi.nlm.nih.gov/pubmed/32944187
http://dx.doi.org/10.1080/20013078.2020.1800222
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