Evanescent Wave Optical Trapping and Sensing on Polymer Optical Fibers for Ultra-Trace Detection of Glucose

[Image: see text] Graphene sensitization of glucose-imprinted polymer (G-IP)-coated optical fiber has been introduced as a new biosensor for evanescent wave trapping on the polymer optical fiber to detect low-level glucose. The developed sensor operates based on the evanescent wave modulation principle. Full characterization via atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy, high-resolution transmission electron microscopy (HRTEM), and N(2) adsorption/desorption of as-prepared G-IP-coated optical fibers was experimentally tested. Accordingly, related operational parameters such as roughness and diameter were optimized. Incorporating graphene into the G-IP not only steadily promotes the electron transport between the fiber surface and as-proposed G-IP but also significantly enhances the sensitivity by acting as a carrier for immobilizing G-IP with specific imprinted cavities. The sensor demonstrates a fast response time (5 s) and high sensitivity, selectivity, and stability, which cause a wide linear range (10–100 nM) and a low limit of detection (LOD = 2.54 nM). Experimental results indicate that the developed sensor facilitates online monitoring and remote sensing of glucose in biological liquids and food samples.