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Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes
BACKGROUND AND OBJECTIVE: The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested. Lactobacillus and Bifidobacterium species influence P. gingivalis interaction with gingival epithelial cells (GECs) but may not act in a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Taylor & Francis
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482675/ https://www.ncbi.nlm.nih.gov/pubmed/32944156 http://dx.doi.org/10.1080/20002297.2020.1805553 |
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author | Ishikawa, Karin Hitomi Mita, Daniela Kawamoto, Dione Nicoli, Jacques Robert Albuquerque-Souza, Emmanuel Lorenzetti Simionato, Maria Regina Mayer, Marcia Pinto Alves |
author_facet | Ishikawa, Karin Hitomi Mita, Daniela Kawamoto, Dione Nicoli, Jacques Robert Albuquerque-Souza, Emmanuel Lorenzetti Simionato, Maria Regina Mayer, Marcia Pinto Alves |
author_sort | Ishikawa, Karin Hitomi |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested. Lactobacillus and Bifidobacterium species influence P. gingivalis interaction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select the most appropriate probiotic against P. gingivalis, we aimed to evaluate the effect of several strains on Porphyromonas gingivalis biofilm formation and transcription virulence-associated factors (PgVAFs). METHODS: Cell-free pH neutralized supernatants (CFS) and living Lactobacillus spp. and Bifidobacterium spp. were tested against P. gingivalis ATCC 33277 and W83, in mono- and multi-species (with Streptococcus oralis and S. gordonii) biofilms. Relative transcription of P. gingivalis genes (fimA, mfa1, kgp, rgp, ftsH and luxS) was determined in biofilms and under GECs co-infection. RESULTS: Probiotics CFS reduced P. gingivalis ATCC 33277 levels in mono-species biofilms and living probiotics reduced P. gingivalis abundance in multi-species biofilms. L. acidophilus LA5 down-regulated transcription of most PgVAFs in biofilms and GECs. CONCLUSIONS: Probiotics affect P. gingivalis biofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed for L. acidophilus LA5. |
format | Online Article Text |
id | pubmed-7482675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-74826752020-09-16 Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes Ishikawa, Karin Hitomi Mita, Daniela Kawamoto, Dione Nicoli, Jacques Robert Albuquerque-Souza, Emmanuel Lorenzetti Simionato, Maria Regina Mayer, Marcia Pinto Alves J Oral Microbiol Original Article BACKGROUND AND OBJECTIVE: The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested. Lactobacillus and Bifidobacterium species influence P. gingivalis interaction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select the most appropriate probiotic against P. gingivalis, we aimed to evaluate the effect of several strains on Porphyromonas gingivalis biofilm formation and transcription virulence-associated factors (PgVAFs). METHODS: Cell-free pH neutralized supernatants (CFS) and living Lactobacillus spp. and Bifidobacterium spp. were tested against P. gingivalis ATCC 33277 and W83, in mono- and multi-species (with Streptococcus oralis and S. gordonii) biofilms. Relative transcription of P. gingivalis genes (fimA, mfa1, kgp, rgp, ftsH and luxS) was determined in biofilms and under GECs co-infection. RESULTS: Probiotics CFS reduced P. gingivalis ATCC 33277 levels in mono-species biofilms and living probiotics reduced P. gingivalis abundance in multi-species biofilms. L. acidophilus LA5 down-regulated transcription of most PgVAFs in biofilms and GECs. CONCLUSIONS: Probiotics affect P. gingivalis biofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed for L. acidophilus LA5. Taylor & Francis 2020-08-20 /pmc/articles/PMC7482675/ /pubmed/32944156 http://dx.doi.org/10.1080/20002297.2020.1805553 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ishikawa, Karin Hitomi Mita, Daniela Kawamoto, Dione Nicoli, Jacques Robert Albuquerque-Souza, Emmanuel Lorenzetti Simionato, Maria Regina Mayer, Marcia Pinto Alves Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes |
title | Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes |
title_full | Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes |
title_fullStr | Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes |
title_full_unstemmed | Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes |
title_short | Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes |
title_sort | probiotics alter biofilm formation and the transcription of porphyromonas gingivalis virulence-associated genes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482675/ https://www.ncbi.nlm.nih.gov/pubmed/32944156 http://dx.doi.org/10.1080/20002297.2020.1805553 |
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