Cargando…
Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains
With more demand for Sabin inactivated poliovirus vaccines (sIPVs) to support the global polio eradication effort worldwide, data regarding the potency characteristics of sIPV after exposure to freezing temperatures are urgently required. In the present study, the sIPVs were stored at −20°C for 24 h...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482872/ https://www.ncbi.nlm.nih.gov/pubmed/32118517 http://dx.doi.org/10.1080/21645515.2019.1709352 |
_version_ | 1783580862036574208 |
---|---|
author | Cai, Wei Ping, Ling Shen, Wuling Liu, Jing Zhang, Ming Zhou, Jian Peng, Jia Wang, Mingqing Zhu, Yun Ji, Guang Wang, Xiaoyu Ji, Qiuyan Lai, Chao Shi, Li Che, Yanchun Sun, Mingbo |
author_facet | Cai, Wei Ping, Ling Shen, Wuling Liu, Jing Zhang, Ming Zhou, Jian Peng, Jia Wang, Mingqing Zhu, Yun Ji, Guang Wang, Xiaoyu Ji, Qiuyan Lai, Chao Shi, Li Che, Yanchun Sun, Mingbo |
author_sort | Cai, Wei |
collection | PubMed |
description | With more demand for Sabin inactivated poliovirus vaccines (sIPVs) to support the global polio eradication effort worldwide, data regarding the potency characteristics of sIPV after exposure to freezing temperatures are urgently required. In the present study, the sIPVs were stored at −20°C for 24 h, 1 week, and 2 weeks in the freezer or in a vaccine carrier for 1 or 3 freeze-thaw cycle to evaluate the effect mediated by freezing temperatures that may be encountered during routine storage and transfer. The in vitro potency was then determined by a D-antigen enzyme-linked immunosorbent assay, and the in vivo potency was evaluated in Wistar rats. In the in vitro study for freezer storage groups, the D-antigen contents for all three types decreased and were lower than the release specifications after storing at −20°C for 2 weeks. After storing at −20°C for 1 week, the D-antigen contents for types I and III in combined group of a total of 45 vials, and for type II in the specific lot groups containing 15 vials decreased, but were within the release specifications. Moreover, no significant change in in vivo potency was observed. For vaccine carrier transfer groups, the D-antigen contents did not decrease after 1 freeze-thaw cycle; in contrast, it decreased, but no significant in vivo potency loss was observed after 3 freeze-thaw cycles. These results suggest that it may be possible to retain sufficient sIPV potency after short periods of freezing or freeze-thawing during transport. |
format | Online Article Text |
id | pubmed-7482872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-74828722020-09-16 Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains Cai, Wei Ping, Ling Shen, Wuling Liu, Jing Zhang, Ming Zhou, Jian Peng, Jia Wang, Mingqing Zhu, Yun Ji, Guang Wang, Xiaoyu Ji, Qiuyan Lai, Chao Shi, Li Che, Yanchun Sun, Mingbo Hum Vaccin Immunother Research Paper With more demand for Sabin inactivated poliovirus vaccines (sIPVs) to support the global polio eradication effort worldwide, data regarding the potency characteristics of sIPV after exposure to freezing temperatures are urgently required. In the present study, the sIPVs were stored at −20°C for 24 h, 1 week, and 2 weeks in the freezer or in a vaccine carrier for 1 or 3 freeze-thaw cycle to evaluate the effect mediated by freezing temperatures that may be encountered during routine storage and transfer. The in vitro potency was then determined by a D-antigen enzyme-linked immunosorbent assay, and the in vivo potency was evaluated in Wistar rats. In the in vitro study for freezer storage groups, the D-antigen contents for all three types decreased and were lower than the release specifications after storing at −20°C for 2 weeks. After storing at −20°C for 1 week, the D-antigen contents for types I and III in combined group of a total of 45 vials, and for type II in the specific lot groups containing 15 vials decreased, but were within the release specifications. Moreover, no significant change in in vivo potency was observed. For vaccine carrier transfer groups, the D-antigen contents did not decrease after 1 freeze-thaw cycle; in contrast, it decreased, but no significant in vivo potency loss was observed after 3 freeze-thaw cycles. These results suggest that it may be possible to retain sufficient sIPV potency after short periods of freezing or freeze-thawing during transport. Taylor & Francis 2020-03-02 /pmc/articles/PMC7482872/ /pubmed/32118517 http://dx.doi.org/10.1080/21645515.2019.1709352 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. |
spellingShingle | Research Paper Cai, Wei Ping, Ling Shen, Wuling Liu, Jing Zhang, Ming Zhou, Jian Peng, Jia Wang, Mingqing Zhu, Yun Ji, Guang Wang, Xiaoyu Ji, Qiuyan Lai, Chao Shi, Li Che, Yanchun Sun, Mingbo Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains |
title | Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains |
title_full | Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains |
title_fullStr | Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains |
title_full_unstemmed | Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains |
title_short | Potency of the Sabin inactivated poliovirus vaccine (sIPV) after exposure to freezing temperatures in cold chains |
title_sort | potency of the sabin inactivated poliovirus vaccine (sipv) after exposure to freezing temperatures in cold chains |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482872/ https://www.ncbi.nlm.nih.gov/pubmed/32118517 http://dx.doi.org/10.1080/21645515.2019.1709352 |
work_keys_str_mv | AT caiwei potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT pingling potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT shenwuling potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT liujing potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT zhangming potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT zhoujian potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT pengjia potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT wangmingqing potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT zhuyun potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT jiguang potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT wangxiaoyu potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT jiqiuyan potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT laichao potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT shili potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT cheyanchun potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains AT sunmingbo potencyofthesabininactivatedpoliovirusvaccinesipvafterexposuretofreezingtemperaturesincoldchains |