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Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children

BACKGROUND: COVID-19, the disease caused by the highly infectious and transmissible coronavirus SARS-CoV-2, has quickly become a morbid global pandemic. Although the impact of SARS-CoV-2 infection in children is less clinically apparent, collecting high-quality biospecimens from infants, children, a...

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Autores principales: Lima, Rosiane, Gootkind, Elizabeth F., De la Flor, Denis, Yockey, Laura J., Bordt, Evan A., D’Avino, Paolo, Ning, Shen, Heath, Katerina, Harding, Katherine, Zois, Jaclyn, Park, Grace, Hardcastle, Margot, Grinke, Kathleen A., Grimmel, Sheila, Davidson, Susan P., Forde, Pamela J., Hall, Kathryn E., Neilan, Anne M., Matute, Juan D., Lerou, Paul H., Fasano, Alessio, Shui, Jessica E., Edlow, Andrea G., Yonker, Lael M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483494/
https://www.ncbi.nlm.nih.gov/pubmed/32917141
http://dx.doi.org/10.1186/s12874-020-01110-y
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author Lima, Rosiane
Gootkind, Elizabeth F.
De la Flor, Denis
Yockey, Laura J.
Bordt, Evan A.
D’Avino, Paolo
Ning, Shen
Heath, Katerina
Harding, Katherine
Zois, Jaclyn
Park, Grace
Hardcastle, Margot
Grinke, Kathleen A.
Grimmel, Sheila
Davidson, Susan P.
Forde, Pamela J.
Hall, Kathryn E.
Neilan, Anne M.
Matute, Juan D.
Lerou, Paul H.
Fasano, Alessio
Shui, Jessica E.
Edlow, Andrea G.
Yonker, Lael M.
author_facet Lima, Rosiane
Gootkind, Elizabeth F.
De la Flor, Denis
Yockey, Laura J.
Bordt, Evan A.
D’Avino, Paolo
Ning, Shen
Heath, Katerina
Harding, Katherine
Zois, Jaclyn
Park, Grace
Hardcastle, Margot
Grinke, Kathleen A.
Grimmel, Sheila
Davidson, Susan P.
Forde, Pamela J.
Hall, Kathryn E.
Neilan, Anne M.
Matute, Juan D.
Lerou, Paul H.
Fasano, Alessio
Shui, Jessica E.
Edlow, Andrea G.
Yonker, Lael M.
author_sort Lima, Rosiane
collection PubMed
description BACKGROUND: COVID-19, the disease caused by the highly infectious and transmissible coronavirus SARS-CoV-2, has quickly become a morbid global pandemic. Although the impact of SARS-CoV-2 infection in children is less clinically apparent, collecting high-quality biospecimens from infants, children, and adolescents in a standardized manner during the COVID-19 pandemic is essential to establish a biologic understanding of the disease in the pediatric population. This biorepository enables pediatric centers world-wide to collect samples uniformly to drive forward our understanding of COVID-19 by addressing specific pediatric and neonatal COVID-19-related questions. METHODS: A COVID-19 biospecimen collection study was implemented with strategic enrollment guidelines to include patients seen in urgent care clinics and hospital settings, neonates born to SARS-CoV-2 infected mothers, and asymptomatic children. The methodology described here, details the importance of establishing collaborations between the clinical and research teams to harmonize protocols for patient recruitment and sample collection, processing and storage. It also details modifications required for biobanking during a surge of the COVID-19 pandemic. RESULTS: Considerations and challenges facing enrollment of neonatal and pediatric cohorts are described. A roadmap is laid out for successful collection, processing, storage and database management of multiple pediatric samples such as blood, nasopharyngeal and oropharyngeal swabs, sputum, saliva, tracheal aspirates, stool, and urine. Using this methodology, we enrolled 327 participants, who provided a total of 972 biospecimens. CONCLUSIONS: Pediatric biospecimens will be key in answering questions relating to viral transmission by children, differences between pediatric and adult viral susceptibility and immune responses, the impact of maternal SARS-CoV-2 infection on fetal development, and factors driving the Multisystem Inflammatory Syndrome in Children. The specimens in this biorepository will allow necessary comparative studies between children and adults, help determine the accuracy of current pediatric viral testing techniques, in addition to, understanding neonatal exposure to SARS-CoV-2 infection and disease abnormalities. The successful establishment of a pediatric biorepository is critical to provide insight into disease pathogenesis, and subsequently, develop future treatment and vaccination strategies.
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spelling pubmed-74834942020-09-11 Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children Lima, Rosiane Gootkind, Elizabeth F. De la Flor, Denis Yockey, Laura J. Bordt, Evan A. D’Avino, Paolo Ning, Shen Heath, Katerina Harding, Katherine Zois, Jaclyn Park, Grace Hardcastle, Margot Grinke, Kathleen A. Grimmel, Sheila Davidson, Susan P. Forde, Pamela J. Hall, Kathryn E. Neilan, Anne M. Matute, Juan D. Lerou, Paul H. Fasano, Alessio Shui, Jessica E. Edlow, Andrea G. Yonker, Lael M. BMC Med Res Methodol Research Article BACKGROUND: COVID-19, the disease caused by the highly infectious and transmissible coronavirus SARS-CoV-2, has quickly become a morbid global pandemic. Although the impact of SARS-CoV-2 infection in children is less clinically apparent, collecting high-quality biospecimens from infants, children, and adolescents in a standardized manner during the COVID-19 pandemic is essential to establish a biologic understanding of the disease in the pediatric population. This biorepository enables pediatric centers world-wide to collect samples uniformly to drive forward our understanding of COVID-19 by addressing specific pediatric and neonatal COVID-19-related questions. METHODS: A COVID-19 biospecimen collection study was implemented with strategic enrollment guidelines to include patients seen in urgent care clinics and hospital settings, neonates born to SARS-CoV-2 infected mothers, and asymptomatic children. The methodology described here, details the importance of establishing collaborations between the clinical and research teams to harmonize protocols for patient recruitment and sample collection, processing and storage. It also details modifications required for biobanking during a surge of the COVID-19 pandemic. RESULTS: Considerations and challenges facing enrollment of neonatal and pediatric cohorts are described. A roadmap is laid out for successful collection, processing, storage and database management of multiple pediatric samples such as blood, nasopharyngeal and oropharyngeal swabs, sputum, saliva, tracheal aspirates, stool, and urine. Using this methodology, we enrolled 327 participants, who provided a total of 972 biospecimens. CONCLUSIONS: Pediatric biospecimens will be key in answering questions relating to viral transmission by children, differences between pediatric and adult viral susceptibility and immune responses, the impact of maternal SARS-CoV-2 infection on fetal development, and factors driving the Multisystem Inflammatory Syndrome in Children. The specimens in this biorepository will allow necessary comparative studies between children and adults, help determine the accuracy of current pediatric viral testing techniques, in addition to, understanding neonatal exposure to SARS-CoV-2 infection and disease abnormalities. The successful establishment of a pediatric biorepository is critical to provide insight into disease pathogenesis, and subsequently, develop future treatment and vaccination strategies. BioMed Central 2020-09-11 /pmc/articles/PMC7483494/ /pubmed/32917141 http://dx.doi.org/10.1186/s12874-020-01110-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Lima, Rosiane
Gootkind, Elizabeth F.
De la Flor, Denis
Yockey, Laura J.
Bordt, Evan A.
D’Avino, Paolo
Ning, Shen
Heath, Katerina
Harding, Katherine
Zois, Jaclyn
Park, Grace
Hardcastle, Margot
Grinke, Kathleen A.
Grimmel, Sheila
Davidson, Susan P.
Forde, Pamela J.
Hall, Kathryn E.
Neilan, Anne M.
Matute, Juan D.
Lerou, Paul H.
Fasano, Alessio
Shui, Jessica E.
Edlow, Andrea G.
Yonker, Lael M.
Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children
title Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children
title_full Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children
title_fullStr Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children
title_full_unstemmed Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children
title_short Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children
title_sort establishment of a pediatric covid-19 biorepository: unique considerations and opportunities for studying the impact of the covid-19 pandemic on children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483494/
https://www.ncbi.nlm.nih.gov/pubmed/32917141
http://dx.doi.org/10.1186/s12874-020-01110-y
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