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Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum
Trypanosoma equiperdum is the causative agent of dourine, a parasitic venereal disease of equids. In this work, rabbits were infected with T. equiperdum strain OVI; serological tests (complement fixation test, ELISA and immunoblotting), used for the diagnosis of dourine in horses, were applied to st...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483502/ https://www.ncbi.nlm.nih.gov/pubmed/32913248 http://dx.doi.org/10.1038/s41598-020-71992-x |
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author | Di Febo, Tiziana Krasteva, Ivanka Bonfini, Barbara Tittarelli, Manuela Matteucci, Osvaldo Orsini, Gianluca Rossi, Emanuela Podaliri Vulpiani, Michele Rodomonti, Diamante Iannetti, Luigi Luciani, Mirella |
author_facet | Di Febo, Tiziana Krasteva, Ivanka Bonfini, Barbara Tittarelli, Manuela Matteucci, Osvaldo Orsini, Gianluca Rossi, Emanuela Podaliri Vulpiani, Michele Rodomonti, Diamante Iannetti, Luigi Luciani, Mirella |
author_sort | Di Febo, Tiziana |
collection | PubMed |
description | Trypanosoma equiperdum is the causative agent of dourine, a parasitic venereal disease of equids. In this work, rabbits were infected with T. equiperdum strain OVI; serological tests (complement fixation test, ELISA and immunoblotting), used for the diagnosis of dourine in horses, were applied to study rabbit humoral immune response and to characterise T. equiperdum antigen pattern recognised by antibodies from infected rabbits. Moreover a protein extract of T. equiperdum strain OVI was produced and tested in skin tests on infected rabbits to detect the cell-mediated response induced by T. equiperdum, in order to evaluate its use in the field diagnosis of dourine. Sera of infected rabbits recognized in immunoblotting Trypanosoma protein bands with molecular weight below 37 kDa, providing a serological response comparable with that already observed in dourine infected horses. Moreover the trypanosome protein extract was capable to produce in vivo delayed-type hypersensitivity (DHT Type IV) in rabbits and proved itself to be non-toxic and non-sensitizing. |
format | Online Article Text |
id | pubmed-7483502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74835022020-09-15 Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum Di Febo, Tiziana Krasteva, Ivanka Bonfini, Barbara Tittarelli, Manuela Matteucci, Osvaldo Orsini, Gianluca Rossi, Emanuela Podaliri Vulpiani, Michele Rodomonti, Diamante Iannetti, Luigi Luciani, Mirella Sci Rep Article Trypanosoma equiperdum is the causative agent of dourine, a parasitic venereal disease of equids. In this work, rabbits were infected with T. equiperdum strain OVI; serological tests (complement fixation test, ELISA and immunoblotting), used for the diagnosis of dourine in horses, were applied to study rabbit humoral immune response and to characterise T. equiperdum antigen pattern recognised by antibodies from infected rabbits. Moreover a protein extract of T. equiperdum strain OVI was produced and tested in skin tests on infected rabbits to detect the cell-mediated response induced by T. equiperdum, in order to evaluate its use in the field diagnosis of dourine. Sera of infected rabbits recognized in immunoblotting Trypanosoma protein bands with molecular weight below 37 kDa, providing a serological response comparable with that already observed in dourine infected horses. Moreover the trypanosome protein extract was capable to produce in vivo delayed-type hypersensitivity (DHT Type IV) in rabbits and proved itself to be non-toxic and non-sensitizing. Nature Publishing Group UK 2020-09-10 /pmc/articles/PMC7483502/ /pubmed/32913248 http://dx.doi.org/10.1038/s41598-020-71992-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Di Febo, Tiziana Krasteva, Ivanka Bonfini, Barbara Tittarelli, Manuela Matteucci, Osvaldo Orsini, Gianluca Rossi, Emanuela Podaliri Vulpiani, Michele Rodomonti, Diamante Iannetti, Luigi Luciani, Mirella Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum |
title | Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum |
title_full | Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum |
title_fullStr | Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum |
title_full_unstemmed | Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum |
title_short | Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum |
title_sort | humoral immune response and delayed-type hypersensitivity in rabbits infected with trypanosoma equiperdum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483502/ https://www.ncbi.nlm.nih.gov/pubmed/32913248 http://dx.doi.org/10.1038/s41598-020-71992-x |
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