Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold

Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation pe...

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Autores principales: Schwartz-Duval, Aaron S., Konopka, Christian J., Moitra, Parikshit, Daza, Enrique A., Srivastava, Indrajit, Johnson, Elyse V., Kampert, Taylor L., Fayn, Stanley, Haran, Anand, Dobrucki, Lawrence W., Pan, Dipanjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483505/
https://www.ncbi.nlm.nih.gov/pubmed/32913195
http://dx.doi.org/10.1038/s41467-020-17595-6
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author Schwartz-Duval, Aaron S.
Konopka, Christian J.
Moitra, Parikshit
Daza, Enrique A.
Srivastava, Indrajit
Johnson, Elyse V.
Kampert, Taylor L.
Fayn, Stanley
Haran, Anand
Dobrucki, Lawrence W.
Pan, Dipanjan
author_facet Schwartz-Duval, Aaron S.
Konopka, Christian J.
Moitra, Parikshit
Daza, Enrique A.
Srivastava, Indrajit
Johnson, Elyse V.
Kampert, Taylor L.
Fayn, Stanley
Haran, Anand
Dobrucki, Lawrence W.
Pan, Dipanjan
author_sort Schwartz-Duval, Aaron S.
collection PubMed
description Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation period of ionic gold. Here, we describe a simplistic method of intracellular biomineralization to produce plasmonic gold nanoparticles at micromolar concentrations within 30 min of application utilizing polyethylene glycol as delivery vector for ionic gold. We have characterized this process for intracellular gold nanoparticle formation, which progressively accumulates proteins as the ionic gold clusters migrate to the nucleus. This nano-vectorized application of ionic gold emphasizes its potential biomedical opportunities while reducing the quantity of ionic gold and required incubation time. To demonstrate its biomedical potential, we further induce in-situ biosynthesis of gold nanoparticles within MCF7 tumor mouse xenografts which is followed by its photothermal remediation.
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spelling pubmed-74835052020-09-21 Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold Schwartz-Duval, Aaron S. Konopka, Christian J. Moitra, Parikshit Daza, Enrique A. Srivastava, Indrajit Johnson, Elyse V. Kampert, Taylor L. Fayn, Stanley Haran, Anand Dobrucki, Lawrence W. Pan, Dipanjan Nat Commun Article Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation period of ionic gold. Here, we describe a simplistic method of intracellular biomineralization to produce plasmonic gold nanoparticles at micromolar concentrations within 30 min of application utilizing polyethylene glycol as delivery vector for ionic gold. We have characterized this process for intracellular gold nanoparticle formation, which progressively accumulates proteins as the ionic gold clusters migrate to the nucleus. This nano-vectorized application of ionic gold emphasizes its potential biomedical opportunities while reducing the quantity of ionic gold and required incubation time. To demonstrate its biomedical potential, we further induce in-situ biosynthesis of gold nanoparticles within MCF7 tumor mouse xenografts which is followed by its photothermal remediation. Nature Publishing Group UK 2020-09-10 /pmc/articles/PMC7483505/ /pubmed/32913195 http://dx.doi.org/10.1038/s41467-020-17595-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schwartz-Duval, Aaron S.
Konopka, Christian J.
Moitra, Parikshit
Daza, Enrique A.
Srivastava, Indrajit
Johnson, Elyse V.
Kampert, Taylor L.
Fayn, Stanley
Haran, Anand
Dobrucki, Lawrence W.
Pan, Dipanjan
Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
title Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
title_full Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
title_fullStr Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
title_full_unstemmed Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
title_short Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
title_sort intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483505/
https://www.ncbi.nlm.nih.gov/pubmed/32913195
http://dx.doi.org/10.1038/s41467-020-17595-6
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