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Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
Benzo[b]fluoranthene (BbF) is one of the main pollutants of polycyclic aromatic hydrocarbons (PAHs), which are generated from organic materials combustion and diesel exhaust. It has been reported that after maternal exposure, BbF crosses the placental barrier, leading to offspring defects. However,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483922/ https://www.ncbi.nlm.nih.gov/pubmed/32982721 http://dx.doi.org/10.3389/fphar.2020.01226 |
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author | Guo, Jing Huang, Jiayu Zhang, Liqun Li, Chong Qin, Yinhua Liu, Weiwei Li, Jingyu Huang, Guoning |
author_facet | Guo, Jing Huang, Jiayu Zhang, Liqun Li, Chong Qin, Yinhua Liu, Weiwei Li, Jingyu Huang, Guoning |
author_sort | Guo, Jing |
collection | PubMed |
description | Benzo[b]fluoranthene (BbF) is one of the main pollutants of polycyclic aromatic hydrocarbons (PAHs), which are generated from organic materials combustion and diesel exhaust. It has been reported that after maternal exposure, BbF crosses the placental barrier, leading to offspring defects. However, the effect of BbF on the female reproductive system, especially on oocyte maturation has not been studied. To elucidate the effect and precise mechanism of BbF on oocyte maturation, nuclear, and cytoplasm maturation were evaluated after exposing mouse oocytes to different concentrations of BbF. Results showed that BbF exposure shows no effect on the meiotic progression, but it caused defects on nuclear maturation via impairment on chromosome alignment. In addition, the treatment of BbF displayed the defects on the cytoplasmic maturation by leading to the mitochondrial dysfunction, DNA damage accumulation, early apoptosis and the loss of H3K4me3. To investigate the mechanism, we found that BbF impaired the oocyte maturation via the AMPK pathway. BbF exposure caused the phosphorylation of AMPK, which cause the DNA damage accumulation and apoptotic incidence. Taken together, our results demonstrated that BbF exposure impaired the mouse oocyte maturation due to mitochondrial dysfunction and early apoptosis. |
format | Online Article Text |
id | pubmed-7483922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74839222020-09-25 Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis Guo, Jing Huang, Jiayu Zhang, Liqun Li, Chong Qin, Yinhua Liu, Weiwei Li, Jingyu Huang, Guoning Front Pharmacol Pharmacology Benzo[b]fluoranthene (BbF) is one of the main pollutants of polycyclic aromatic hydrocarbons (PAHs), which are generated from organic materials combustion and diesel exhaust. It has been reported that after maternal exposure, BbF crosses the placental barrier, leading to offspring defects. However, the effect of BbF on the female reproductive system, especially on oocyte maturation has not been studied. To elucidate the effect and precise mechanism of BbF on oocyte maturation, nuclear, and cytoplasm maturation were evaluated after exposing mouse oocytes to different concentrations of BbF. Results showed that BbF exposure shows no effect on the meiotic progression, but it caused defects on nuclear maturation via impairment on chromosome alignment. In addition, the treatment of BbF displayed the defects on the cytoplasmic maturation by leading to the mitochondrial dysfunction, DNA damage accumulation, early apoptosis and the loss of H3K4me3. To investigate the mechanism, we found that BbF impaired the oocyte maturation via the AMPK pathway. BbF exposure caused the phosphorylation of AMPK, which cause the DNA damage accumulation and apoptotic incidence. Taken together, our results demonstrated that BbF exposure impaired the mouse oocyte maturation due to mitochondrial dysfunction and early apoptosis. Frontiers Media S.A. 2020-08-28 /pmc/articles/PMC7483922/ /pubmed/32982721 http://dx.doi.org/10.3389/fphar.2020.01226 Text en Copyright © 2020 Guo, Huang, Zhang, Li, Qin, Liu, Li and Huang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Guo, Jing Huang, Jiayu Zhang, Liqun Li, Chong Qin, Yinhua Liu, Weiwei Li, Jingyu Huang, Guoning Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis |
title | Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis |
title_full | Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis |
title_fullStr | Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis |
title_full_unstemmed | Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis |
title_short | Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis |
title_sort | benzo[b]fluoranthene impairs mouse oocyte maturation via inducing the apoptosis |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483922/ https://www.ncbi.nlm.nih.gov/pubmed/32982721 http://dx.doi.org/10.3389/fphar.2020.01226 |
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