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Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis

Benzo[b]fluoranthene (BbF) is one of the main pollutants of polycyclic aromatic hydrocarbons (PAHs), which are generated from organic materials combustion and diesel exhaust. It has been reported that after maternal exposure, BbF crosses the placental barrier, leading to offspring defects. However,...

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Autores principales: Guo, Jing, Huang, Jiayu, Zhang, Liqun, Li, Chong, Qin, Yinhua, Liu, Weiwei, Li, Jingyu, Huang, Guoning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483922/
https://www.ncbi.nlm.nih.gov/pubmed/32982721
http://dx.doi.org/10.3389/fphar.2020.01226
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author Guo, Jing
Huang, Jiayu
Zhang, Liqun
Li, Chong
Qin, Yinhua
Liu, Weiwei
Li, Jingyu
Huang, Guoning
author_facet Guo, Jing
Huang, Jiayu
Zhang, Liqun
Li, Chong
Qin, Yinhua
Liu, Weiwei
Li, Jingyu
Huang, Guoning
author_sort Guo, Jing
collection PubMed
description Benzo[b]fluoranthene (BbF) is one of the main pollutants of polycyclic aromatic hydrocarbons (PAHs), which are generated from organic materials combustion and diesel exhaust. It has been reported that after maternal exposure, BbF crosses the placental barrier, leading to offspring defects. However, the effect of BbF on the female reproductive system, especially on oocyte maturation has not been studied. To elucidate the effect and precise mechanism of BbF on oocyte maturation, nuclear, and cytoplasm maturation were evaluated after exposing mouse oocytes to different concentrations of BbF. Results showed that BbF exposure shows no effect on the meiotic progression, but it caused defects on nuclear maturation via impairment on chromosome alignment. In addition, the treatment of BbF displayed the defects on the cytoplasmic maturation by leading to the mitochondrial dysfunction, DNA damage accumulation, early apoptosis and the loss of H3K4me3. To investigate the mechanism, we found that BbF impaired the oocyte maturation via the AMPK pathway. BbF exposure caused the phosphorylation of AMPK, which cause the DNA damage accumulation and apoptotic incidence. Taken together, our results demonstrated that BbF exposure impaired the mouse oocyte maturation due to mitochondrial dysfunction and early apoptosis.
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spelling pubmed-74839222020-09-25 Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis Guo, Jing Huang, Jiayu Zhang, Liqun Li, Chong Qin, Yinhua Liu, Weiwei Li, Jingyu Huang, Guoning Front Pharmacol Pharmacology Benzo[b]fluoranthene (BbF) is one of the main pollutants of polycyclic aromatic hydrocarbons (PAHs), which are generated from organic materials combustion and diesel exhaust. It has been reported that after maternal exposure, BbF crosses the placental barrier, leading to offspring defects. However, the effect of BbF on the female reproductive system, especially on oocyte maturation has not been studied. To elucidate the effect and precise mechanism of BbF on oocyte maturation, nuclear, and cytoplasm maturation were evaluated after exposing mouse oocytes to different concentrations of BbF. Results showed that BbF exposure shows no effect on the meiotic progression, but it caused defects on nuclear maturation via impairment on chromosome alignment. In addition, the treatment of BbF displayed the defects on the cytoplasmic maturation by leading to the mitochondrial dysfunction, DNA damage accumulation, early apoptosis and the loss of H3K4me3. To investigate the mechanism, we found that BbF impaired the oocyte maturation via the AMPK pathway. BbF exposure caused the phosphorylation of AMPK, which cause the DNA damage accumulation and apoptotic incidence. Taken together, our results demonstrated that BbF exposure impaired the mouse oocyte maturation due to mitochondrial dysfunction and early apoptosis. Frontiers Media S.A. 2020-08-28 /pmc/articles/PMC7483922/ /pubmed/32982721 http://dx.doi.org/10.3389/fphar.2020.01226 Text en Copyright © 2020 Guo, Huang, Zhang, Li, Qin, Liu, Li and Huang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Guo, Jing
Huang, Jiayu
Zhang, Liqun
Li, Chong
Qin, Yinhua
Liu, Weiwei
Li, Jingyu
Huang, Guoning
Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
title Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
title_full Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
title_fullStr Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
title_full_unstemmed Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
title_short Benzo[b]fluoranthene Impairs Mouse Oocyte Maturation via Inducing the Apoptosis
title_sort benzo[b]fluoranthene impairs mouse oocyte maturation via inducing the apoptosis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483922/
https://www.ncbi.nlm.nih.gov/pubmed/32982721
http://dx.doi.org/10.3389/fphar.2020.01226
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