Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones

We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla geno...

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Autores principales: Kitano, Takashi, Kim, Choong-Gon, Saitou, Naruya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484619/
https://www.ncbi.nlm.nih.gov/pubmed/32934753
http://dx.doi.org/10.7150/jgen.50468
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author Kitano, Takashi
Kim, Choong-Gon
Saitou, Naruya
author_facet Kitano, Takashi
Kim, Choong-Gon
Saitou, Naruya
author_sort Kitano, Takashi
collection PubMed
description We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.
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spelling pubmed-74846192020-09-14 Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones Kitano, Takashi Kim, Choong-Gon Saitou, Naruya J Genomics Research Paper We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas. Ivyspring International Publisher 2020-08-29 /pmc/articles/PMC7484619/ /pubmed/32934753 http://dx.doi.org/10.7150/jgen.50468 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Kitano, Takashi
Kim, Choong-Gon
Saitou, Naruya
Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
title Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
title_full Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
title_fullStr Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
title_full_unstemmed Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
title_short Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
title_sort nucleotide sequencing of the hoxa gene cluster using gorilla fosmid clones
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484619/
https://www.ncbi.nlm.nih.gov/pubmed/32934753
http://dx.doi.org/10.7150/jgen.50468
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AT saitounaruya nucleotidesequencingofthehoxageneclusterusinggorillafosmidclones

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