Introduction

The major advancements in biomedical research over the past 30 years have been based in the broad field of molecular biology. DNA, RNA, and proteins are now routinely sequenced, altered, and their functions analyzed in great detail. The in situ-based methodologies (immunohistochemistry and in situ hybridization) were in their infancy 30 years ago. Today, they are mainstream, as evidenced by the tens of thousands of peer review articles published each year that have used either methodology. Still, much remains to be learned about how to maximize the power of immunohistochemistry and in situ hybridization. This book starts by building a foundation, assuming little or no prior knowledge in the area of molecular biology. I hope that, at the end of the book, you will have a fount of knowledge that allows you to be very knowledgeable and capable in the in situ-based molecular pathology methods.