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Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a me...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485163/ https://www.ncbi.nlm.nih.gov/pubmed/32952396 http://dx.doi.org/10.1177/1177271920954828 |
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author | Álvarez-Rodríguez, Ignacio I Castaño-Tostado, Eduardo García-Gutiérrez, David G Reynoso-Camacho, Rosalía Elton-Puente, Juana E Barajas-Pozos, Alicia Pérez-Ramírez, Iza F |
author_facet | Álvarez-Rodríguez, Ignacio I Castaño-Tostado, Eduardo García-Gutiérrez, David G Reynoso-Camacho, Rosalía Elton-Puente, Juana E Barajas-Pozos, Alicia Pérez-Ramírez, Iza F |
author_sort | Álvarez-Rodríguez, Ignacio I |
collection | PubMed |
description | Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a metabolomic analysis of serum samples obtained from T2DM adult patients diagnosed with diabetic foot ulcer in a cross-sectional, observational, and comparative study. Eighty-four volunteers were classified into the following groups: without T2DM (control group, n = 30) and with T2DM and different stages of diabetic foot ulcer according to Wagner-Meggitt classification system: DFU G0 (n = 11), DFU G1 (n = 14), DFU G2 (n = 16), and DFU G3 (n = 13). The non-target metabolomic profile followed by chemometric analysis revealed that lysophosphatidylethanolamine (16:1) could be proposed as key metabolite related to the onset of diabetic foot ulcer; however, this phospholipid was not affected by diabetic foot ulcer progression. Therefore, further studies are necessary to validate these phospholipids as biomarker candidates for the early diagnosis of diabetic foot ulcer in T2DM patients. |
format | Online Article Text |
id | pubmed-7485163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-74851632020-09-17 Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer Álvarez-Rodríguez, Ignacio I Castaño-Tostado, Eduardo García-Gutiérrez, David G Reynoso-Camacho, Rosalía Elton-Puente, Juana E Barajas-Pozos, Alicia Pérez-Ramírez, Iza F Biomark Insights Original Research Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a metabolomic analysis of serum samples obtained from T2DM adult patients diagnosed with diabetic foot ulcer in a cross-sectional, observational, and comparative study. Eighty-four volunteers were classified into the following groups: without T2DM (control group, n = 30) and with T2DM and different stages of diabetic foot ulcer according to Wagner-Meggitt classification system: DFU G0 (n = 11), DFU G1 (n = 14), DFU G2 (n = 16), and DFU G3 (n = 13). The non-target metabolomic profile followed by chemometric analysis revealed that lysophosphatidylethanolamine (16:1) could be proposed as key metabolite related to the onset of diabetic foot ulcer; however, this phospholipid was not affected by diabetic foot ulcer progression. Therefore, further studies are necessary to validate these phospholipids as biomarker candidates for the early diagnosis of diabetic foot ulcer in T2DM patients. SAGE Publications 2020-09-09 /pmc/articles/PMC7485163/ /pubmed/32952396 http://dx.doi.org/10.1177/1177271920954828 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Álvarez-Rodríguez, Ignacio I Castaño-Tostado, Eduardo García-Gutiérrez, David G Reynoso-Camacho, Rosalía Elton-Puente, Juana E Barajas-Pozos, Alicia Pérez-Ramírez, Iza F Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer |
title | Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid
Alterations in Patients with Early Stages of Diabetic Foot Ulcer |
title_full | Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid
Alterations in Patients with Early Stages of Diabetic Foot Ulcer |
title_fullStr | Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid
Alterations in Patients with Early Stages of Diabetic Foot Ulcer |
title_full_unstemmed | Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid
Alterations in Patients with Early Stages of Diabetic Foot Ulcer |
title_short | Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid
Alterations in Patients with Early Stages of Diabetic Foot Ulcer |
title_sort | non-targeted metabolomic analysis reveals serum phospholipid
alterations in patients with early stages of diabetic foot ulcer |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485163/ https://www.ncbi.nlm.nih.gov/pubmed/32952396 http://dx.doi.org/10.1177/1177271920954828 |
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