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Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer

Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a me...

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Autores principales: Álvarez-Rodríguez, Ignacio I, Castaño-Tostado, Eduardo, García-Gutiérrez, David G, Reynoso-Camacho, Rosalía, Elton-Puente, Juana E, Barajas-Pozos, Alicia, Pérez-Ramírez, Iza F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485163/
https://www.ncbi.nlm.nih.gov/pubmed/32952396
http://dx.doi.org/10.1177/1177271920954828
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author Álvarez-Rodríguez, Ignacio I
Castaño-Tostado, Eduardo
García-Gutiérrez, David G
Reynoso-Camacho, Rosalía
Elton-Puente, Juana E
Barajas-Pozos, Alicia
Pérez-Ramírez, Iza F
author_facet Álvarez-Rodríguez, Ignacio I
Castaño-Tostado, Eduardo
García-Gutiérrez, David G
Reynoso-Camacho, Rosalía
Elton-Puente, Juana E
Barajas-Pozos, Alicia
Pérez-Ramírez, Iza F
author_sort Álvarez-Rodríguez, Ignacio I
collection PubMed
description Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a metabolomic analysis of serum samples obtained from T2DM adult patients diagnosed with diabetic foot ulcer in a cross-sectional, observational, and comparative study. Eighty-four volunteers were classified into the following groups: without T2DM (control group, n = 30) and with T2DM and different stages of diabetic foot ulcer according to Wagner-Meggitt classification system: DFU G0 (n = 11), DFU G1 (n = 14), DFU G2 (n = 16), and DFU G3 (n = 13). The non-target metabolomic profile followed by chemometric analysis revealed that lysophosphatidylethanolamine (16:1) could be proposed as key metabolite related to the onset of diabetic foot ulcer; however, this phospholipid was not affected by diabetic foot ulcer progression. Therefore, further studies are necessary to validate these phospholipids as biomarker candidates for the early diagnosis of diabetic foot ulcer in T2DM patients.
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spelling pubmed-74851632020-09-17 Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer Álvarez-Rodríguez, Ignacio I Castaño-Tostado, Eduardo García-Gutiérrez, David G Reynoso-Camacho, Rosalía Elton-Puente, Juana E Barajas-Pozos, Alicia Pérez-Ramírez, Iza F Biomark Insights Original Research Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a metabolomic analysis of serum samples obtained from T2DM adult patients diagnosed with diabetic foot ulcer in a cross-sectional, observational, and comparative study. Eighty-four volunteers were classified into the following groups: without T2DM (control group, n = 30) and with T2DM and different stages of diabetic foot ulcer according to Wagner-Meggitt classification system: DFU G0 (n = 11), DFU G1 (n = 14), DFU G2 (n = 16), and DFU G3 (n = 13). The non-target metabolomic profile followed by chemometric analysis revealed that lysophosphatidylethanolamine (16:1) could be proposed as key metabolite related to the onset of diabetic foot ulcer; however, this phospholipid was not affected by diabetic foot ulcer progression. Therefore, further studies are necessary to validate these phospholipids as biomarker candidates for the early diagnosis of diabetic foot ulcer in T2DM patients. SAGE Publications 2020-09-09 /pmc/articles/PMC7485163/ /pubmed/32952396 http://dx.doi.org/10.1177/1177271920954828 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Álvarez-Rodríguez, Ignacio I
Castaño-Tostado, Eduardo
García-Gutiérrez, David G
Reynoso-Camacho, Rosalía
Elton-Puente, Juana E
Barajas-Pozos, Alicia
Pérez-Ramírez, Iza F
Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
title Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
title_full Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
title_fullStr Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
title_full_unstemmed Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
title_short Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer
title_sort non-targeted metabolomic analysis reveals serum phospholipid alterations in patients with early stages of diabetic foot ulcer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485163/
https://www.ncbi.nlm.nih.gov/pubmed/32952396
http://dx.doi.org/10.1177/1177271920954828
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