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Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models

Falcarindiol (FaDOH) is a cytotoxic and anti-inflammatory polyacetylenic oxylipin found in food plants of the carrot family (Apiaceae). FaDOH has been shown to activate PPARγ and to increase the expression of the cholesterol transporter ABCA1 in cells, both of which play an important role in lipid m...

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Autores principales: Andersen, Camilla Bertel, Runge Walther, Anders, Pipó-Ollé, Emma, Notabi, Martine K., Juul, Sebastian, Eriksen, Mathias Hessellund, Lovatt, Adam Leslie, Cowie, Richard, Linnet, Jes, Kobaek-Larsen, Morten, El-Houri, Rime, Andersen, Morten Østergaard, Hedegaard, Martin Aage Barsøe, Christensen, Lars Porskjær, Arnspang, Eva Christensen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485416/
https://www.ncbi.nlm.nih.gov/pubmed/32982759
http://dx.doi.org/10.3389/fphar.2020.565524
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author Andersen, Camilla Bertel
Runge Walther, Anders
Pipó-Ollé, Emma
Notabi, Martine K.
Juul, Sebastian
Eriksen, Mathias Hessellund
Lovatt, Adam Leslie
Cowie, Richard
Linnet, Jes
Kobaek-Larsen, Morten
El-Houri, Rime
Andersen, Morten Østergaard
Hedegaard, Martin Aage Barsøe
Christensen, Lars Porskjær
Arnspang, Eva Christensen
author_facet Andersen, Camilla Bertel
Runge Walther, Anders
Pipó-Ollé, Emma
Notabi, Martine K.
Juul, Sebastian
Eriksen, Mathias Hessellund
Lovatt, Adam Leslie
Cowie, Richard
Linnet, Jes
Kobaek-Larsen, Morten
El-Houri, Rime
Andersen, Morten Østergaard
Hedegaard, Martin Aage Barsøe
Christensen, Lars Porskjær
Arnspang, Eva Christensen
author_sort Andersen, Camilla Bertel
collection PubMed
description Falcarindiol (FaDOH) is a cytotoxic and anti-inflammatory polyacetylenic oxylipin found in food plants of the carrot family (Apiaceae). FaDOH has been shown to activate PPARγ and to increase the expression of the cholesterol transporter ABCA1 in cells, both of which play an important role in lipid metabolism. Thus, a common mechanism of action of the anticancer and antidiabetic properties of FaDOH may be due to a possible effect on lipid metabolism. In this study, the effect of sub-toxic concentration (5 μM) of FaDOH inside human mesenchymal stem cells (hMSCs) was studied using white light microscopy and Raman imaging. Our results show that FaDOH increases lipid content in the hMSCs cells as well as the number of lipid droplets (LDs) and that this can be explained by increased expression of PPARγ2 as shown in human colon adenocarcinoma cells. Activation of PPARγ can lead to increased expression of ABCA1. We demonstrate that ABCA1 is upregulated in colorectal neoplastic rat tissue, which indicates a possible role of this transporter in the redistribution of lipids and increased formation of LDs in cancer cells that may lead to endoplasmic reticulum stress and cancer cell death.
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spelling pubmed-74854162020-09-24 Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models Andersen, Camilla Bertel Runge Walther, Anders Pipó-Ollé, Emma Notabi, Martine K. Juul, Sebastian Eriksen, Mathias Hessellund Lovatt, Adam Leslie Cowie, Richard Linnet, Jes Kobaek-Larsen, Morten El-Houri, Rime Andersen, Morten Østergaard Hedegaard, Martin Aage Barsøe Christensen, Lars Porskjær Arnspang, Eva Christensen Front Pharmacol Pharmacology Falcarindiol (FaDOH) is a cytotoxic and anti-inflammatory polyacetylenic oxylipin found in food plants of the carrot family (Apiaceae). FaDOH has been shown to activate PPARγ and to increase the expression of the cholesterol transporter ABCA1 in cells, both of which play an important role in lipid metabolism. Thus, a common mechanism of action of the anticancer and antidiabetic properties of FaDOH may be due to a possible effect on lipid metabolism. In this study, the effect of sub-toxic concentration (5 μM) of FaDOH inside human mesenchymal stem cells (hMSCs) was studied using white light microscopy and Raman imaging. Our results show that FaDOH increases lipid content in the hMSCs cells as well as the number of lipid droplets (LDs) and that this can be explained by increased expression of PPARγ2 as shown in human colon adenocarcinoma cells. Activation of PPARγ can lead to increased expression of ABCA1. We demonstrate that ABCA1 is upregulated in colorectal neoplastic rat tissue, which indicates a possible role of this transporter in the redistribution of lipids and increased formation of LDs in cancer cells that may lead to endoplasmic reticulum stress and cancer cell death. Frontiers Media S.A. 2020-08-28 /pmc/articles/PMC7485416/ /pubmed/32982759 http://dx.doi.org/10.3389/fphar.2020.565524 Text en Copyright © 2020 Andersen, Runge Walther, Pipó-Ollé, Notabi, Juul, Eriksen, Lovatt, Cowie, Linnet, Kobaek-Larsen, El-Houri, Andersen, Hedegaard, Christensen and Arnspang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Andersen, Camilla Bertel
Runge Walther, Anders
Pipó-Ollé, Emma
Notabi, Martine K.
Juul, Sebastian
Eriksen, Mathias Hessellund
Lovatt, Adam Leslie
Cowie, Richard
Linnet, Jes
Kobaek-Larsen, Morten
El-Houri, Rime
Andersen, Morten Østergaard
Hedegaard, Martin Aage Barsøe
Christensen, Lars Porskjær
Arnspang, Eva Christensen
Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
title Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
title_full Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
title_fullStr Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
title_full_unstemmed Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
title_short Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
title_sort falcarindiol purified from carrots leads to elevated levels of lipid droplets and upregulation of peroxisome proliferator-activated receptor-γ gene expression in cellular models
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485416/
https://www.ncbi.nlm.nih.gov/pubmed/32982759
http://dx.doi.org/10.3389/fphar.2020.565524
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