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KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression
The incidence of thyroid cancer remains high worldwide, and papillary thyroid cancer (PTC) is the most common type. Potassium Calcium-Activated Channel Subfamily N Member 4 (KCNN4) has been reported as an oncogene in various cancers. We examined expression of KCNN4 in public databases and discovered...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485723/ https://www.ncbi.nlm.nih.gov/pubmed/32857728 http://dx.doi.org/10.18632/aging.103710 |
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author | Wen, Jialiang Lin, Bangyi Lin, Lizhi Chen, Yizuo Wang, Ouchen |
author_facet | Wen, Jialiang Lin, Bangyi Lin, Lizhi Chen, Yizuo Wang, Ouchen |
author_sort | Wen, Jialiang |
collection | PubMed |
description | The incidence of thyroid cancer remains high worldwide, and papillary thyroid cancer (PTC) is the most common type. Potassium Calcium-Activated Channel Subfamily N Member 4 (KCNN4) has been reported as an oncogene in various cancers. We examined expression of KCNN4 in public databases and discovered that it is upregulated in PTC. We verified this finding using our own validated cohort and RNA sequencing data. We also found that KCNN4 is a diagnostic and prognostic biomarker that is associated with disease-free survival, immune infiltration, and several other clinicopathological features of PTC. Gene Set Enrichment Analysis indicated that apoptotic and epithelial-mesenchymal transition gene sets are both upregulated in PTC patients with higher KCNN4 levels. In PTC cell lines, silencing KCNN4 inhibited cell proliferation, migration and invasion. Moreover, quantitative real-time PCR and Western blotting indicated that silencing KCNN4 increased expression of apoptotic genes in PTC cells and reduced the expression of genes involved in their epithelial-mesenchymal transition. These results suggest that KCNN4 promotes PTC progression by inducing epithelial-mesenchymal transition and suppressing apoptosis, which suggests KCNN4 may be a useful diagnostic and prognostic biomarker of PTC. |
format | Online Article Text |
id | pubmed-7485723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-74857232020-09-14 KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression Wen, Jialiang Lin, Bangyi Lin, Lizhi Chen, Yizuo Wang, Ouchen Aging (Albany NY) Research Paper The incidence of thyroid cancer remains high worldwide, and papillary thyroid cancer (PTC) is the most common type. Potassium Calcium-Activated Channel Subfamily N Member 4 (KCNN4) has been reported as an oncogene in various cancers. We examined expression of KCNN4 in public databases and discovered that it is upregulated in PTC. We verified this finding using our own validated cohort and RNA sequencing data. We also found that KCNN4 is a diagnostic and prognostic biomarker that is associated with disease-free survival, immune infiltration, and several other clinicopathological features of PTC. Gene Set Enrichment Analysis indicated that apoptotic and epithelial-mesenchymal transition gene sets are both upregulated in PTC patients with higher KCNN4 levels. In PTC cell lines, silencing KCNN4 inhibited cell proliferation, migration and invasion. Moreover, quantitative real-time PCR and Western blotting indicated that silencing KCNN4 increased expression of apoptotic genes in PTC cells and reduced the expression of genes involved in their epithelial-mesenchymal transition. These results suggest that KCNN4 promotes PTC progression by inducing epithelial-mesenchymal transition and suppressing apoptosis, which suggests KCNN4 may be a useful diagnostic and prognostic biomarker of PTC. Impact Journals 2020-08-28 /pmc/articles/PMC7485723/ /pubmed/32857728 http://dx.doi.org/10.18632/aging.103710 Text en Copyright © 2020 Wen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Wen, Jialiang Lin, Bangyi Lin, Lizhi Chen, Yizuo Wang, Ouchen KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
title | KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
title_full | KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
title_fullStr | KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
title_full_unstemmed | KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
title_short | KCNN4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
title_sort | kcnn4 is a diagnostic and prognostic biomarker that promotes papillary thyroid cancer progression |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485723/ https://www.ncbi.nlm.nih.gov/pubmed/32857728 http://dx.doi.org/10.18632/aging.103710 |
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