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Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age
Telomeres are transcribed into long non-coding RNAs known as Telomeric Repeat-Containing RNA (TERRA). They have been shown to be essential regulators of telomeres and to act as epigenomic modulators at extra-telomeric sites. However the role of TERRA during early embryonic development has never been...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485725/ https://www.ncbi.nlm.nih.gov/pubmed/32860669 http://dx.doi.org/10.18632/aging.103922 |
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author | Kordowitzki, Paweł López de Silanes, Isabel Guío-Carrión, Ana Blasco, Maria A. |
author_facet | Kordowitzki, Paweł López de Silanes, Isabel Guío-Carrión, Ana Blasco, Maria A. |
author_sort | Kordowitzki, Paweł |
collection | PubMed |
description | Telomeres are transcribed into long non-coding RNAs known as Telomeric Repeat-Containing RNA (TERRA). They have been shown to be essential regulators of telomeres and to act as epigenomic modulators at extra-telomeric sites. However the role of TERRA during early embryonic development has never been investigated. Here, we show that TERRA is expressed in murine and bovine early development following a wave pattern. It starts at 4-cell stage, reaching a maximum at the 16-cell followed by a decline at the morula and blastocyst stages. Moreover, TERRA expression is not affected by increasing oocyte donor age whereas telomere length does. This indicates that TERRA expression is independent of the telomere length in early development. Our findings anticipate an essential role of TERRA in early stages of development and this might be useful in the future for a better understanding of age related female infertility. |
format | Online Article Text |
id | pubmed-7485725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-74857252020-09-14 Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age Kordowitzki, Paweł López de Silanes, Isabel Guío-Carrión, Ana Blasco, Maria A. Aging (Albany NY) Priority Research Paper Telomeres are transcribed into long non-coding RNAs known as Telomeric Repeat-Containing RNA (TERRA). They have been shown to be essential regulators of telomeres and to act as epigenomic modulators at extra-telomeric sites. However the role of TERRA during early embryonic development has never been investigated. Here, we show that TERRA is expressed in murine and bovine early development following a wave pattern. It starts at 4-cell stage, reaching a maximum at the 16-cell followed by a decline at the morula and blastocyst stages. Moreover, TERRA expression is not affected by increasing oocyte donor age whereas telomere length does. This indicates that TERRA expression is independent of the telomere length in early development. Our findings anticipate an essential role of TERRA in early stages of development and this might be useful in the future for a better understanding of age related female infertility. Impact Journals 2020-08-29 /pmc/articles/PMC7485725/ /pubmed/32860669 http://dx.doi.org/10.18632/aging.103922 Text en Copyright © 2020 Kordowitzki et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited. |
spellingShingle | Priority Research Paper Kordowitzki, Paweł López de Silanes, Isabel Guío-Carrión, Ana Blasco, Maria A. Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age |
title | Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age |
title_full | Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age |
title_fullStr | Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age |
title_full_unstemmed | Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age |
title_short | Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age |
title_sort | dynamics of telomeric repeat-containing rna expression in early embryonic cleavage stages with regards to maternal age |
topic | Priority Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485725/ https://www.ncbi.nlm.nih.gov/pubmed/32860669 http://dx.doi.org/10.18632/aging.103922 |
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