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Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis

BACKGROUND: Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lung...

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Autores principales: Nielsen, Bibi Uhre, Kolpen, Mette, Jensen, Peter Østrup, Katzenstein, Terese, Pressler, Tacjana, Ritz, Christian, Mathiesen, Inger Hee Mabuza, Faurholt-Jepsen, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485830/
https://www.ncbi.nlm.nih.gov/pubmed/32915806
http://dx.doi.org/10.1371/journal.pone.0238524
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author Nielsen, Bibi Uhre
Kolpen, Mette
Jensen, Peter Østrup
Katzenstein, Terese
Pressler, Tacjana
Ritz, Christian
Mathiesen, Inger Hee Mabuza
Faurholt-Jepsen, Daniel
author_facet Nielsen, Bibi Uhre
Kolpen, Mette
Jensen, Peter Østrup
Katzenstein, Terese
Pressler, Tacjana
Ritz, Christian
Mathiesen, Inger Hee Mabuza
Faurholt-Jepsen, Daniel
author_sort Nielsen, Bibi Uhre
collection PubMed
description BACKGROUND: Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lungs. We aimed to investigate changes in and associations between PMNs, glucose and L-lactate in sputum during antibiotic treatment. In addition, the effect of hemoglobin A1c and plasma glucose on these biomarkers were investigated. METHODS: We sampled non-induced sputum at day 0, 7, 14 and 42 in 27 chronically infected CF patients electively treated with 14 days of intravenous antibiotic. To analyze sputum samples, we used flowcytometry to count PMNs and colorimetric assays to estimate lactate and glucose. RESULTS: No changes in levels of PMNs, glucose and lactate were detected in sputum during the antibiotic treatment. Sputum PMNs were positively associated with both glucose (log coefficient = 0.20, p = 0.01) and L-lactate (log coefficient = 0.34, p<0.001). In multivariate analyses, hemoglobin A1c was negatively associated with sputum PMNs (log coefficient = -1.68, p<0.001) and plasma glucose was negatively associated with sputum glucose (log coefficient = -0.09, p = 0.02). CONCLUSIONS: In CF sputum PMNs, glucose and lactate were unchanged during elective antibiotic treatment. However, sputum PMNs were associated with both sputum glucose and sputum lactate. Surprisingly, hyperglycemia seemed to be associated with less PMNs infiltration and less glucose in CF sputum.
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spelling pubmed-74858302020-09-21 Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis Nielsen, Bibi Uhre Kolpen, Mette Jensen, Peter Østrup Katzenstein, Terese Pressler, Tacjana Ritz, Christian Mathiesen, Inger Hee Mabuza Faurholt-Jepsen, Daniel PLoS One Research Article BACKGROUND: Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lungs. We aimed to investigate changes in and associations between PMNs, glucose and L-lactate in sputum during antibiotic treatment. In addition, the effect of hemoglobin A1c and plasma glucose on these biomarkers were investigated. METHODS: We sampled non-induced sputum at day 0, 7, 14 and 42 in 27 chronically infected CF patients electively treated with 14 days of intravenous antibiotic. To analyze sputum samples, we used flowcytometry to count PMNs and colorimetric assays to estimate lactate and glucose. RESULTS: No changes in levels of PMNs, glucose and lactate were detected in sputum during the antibiotic treatment. Sputum PMNs were positively associated with both glucose (log coefficient = 0.20, p = 0.01) and L-lactate (log coefficient = 0.34, p<0.001). In multivariate analyses, hemoglobin A1c was negatively associated with sputum PMNs (log coefficient = -1.68, p<0.001) and plasma glucose was negatively associated with sputum glucose (log coefficient = -0.09, p = 0.02). CONCLUSIONS: In CF sputum PMNs, glucose and lactate were unchanged during elective antibiotic treatment. However, sputum PMNs were associated with both sputum glucose and sputum lactate. Surprisingly, hyperglycemia seemed to be associated with less PMNs infiltration and less glucose in CF sputum. Public Library of Science 2020-09-11 /pmc/articles/PMC7485830/ /pubmed/32915806 http://dx.doi.org/10.1371/journal.pone.0238524 Text en © 2020 Nielsen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nielsen, Bibi Uhre
Kolpen, Mette
Jensen, Peter Østrup
Katzenstein, Terese
Pressler, Tacjana
Ritz, Christian
Mathiesen, Inger Hee Mabuza
Faurholt-Jepsen, Daniel
Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis
title Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis
title_full Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis
title_fullStr Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis
title_full_unstemmed Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis
title_short Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis
title_sort neutrophil count in sputum is associated with increased sputum glucose and sputum l-lactate in cystic fibrosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485830/
https://www.ncbi.nlm.nih.gov/pubmed/32915806
http://dx.doi.org/10.1371/journal.pone.0238524
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