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Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium

Oxidative injury is implicated in retinal damage observed in age-related macular degeneration (AMD), as well as other degenerative conditions. Abnormally elevated levels of iron accumulation within the retinal pigment epithelium have been detected in eyes with AMD, and it is suspected to play a role...

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Autores principales: Kieffer, Jason, Singh, Sushant, Dhillon, Baltej S, Kumar, Udit, Shaikh, Saad, Ho, Son, Seal, Sudipta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485992/
https://www.ncbi.nlm.nih.gov/pubmed/32923270
http://dx.doi.org/10.7759/cureus.9675
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author Kieffer, Jason
Singh, Sushant
Dhillon, Baltej S
Kumar, Udit
Shaikh, Saad
Ho, Son
Seal, Sudipta
author_facet Kieffer, Jason
Singh, Sushant
Dhillon, Baltej S
Kumar, Udit
Shaikh, Saad
Ho, Son
Seal, Sudipta
author_sort Kieffer, Jason
collection PubMed
description Oxidative injury is implicated in retinal damage observed in age-related macular degeneration (AMD), as well as other degenerative conditions. Abnormally elevated levels of iron accumulation within the retinal pigment epithelium have been detected in eyes with AMD, and it is suspected to play a role in the pathogenesis through the production of reactive oxygen species (ROS). Ceria nanoparticles (CNP) have the ability to scavenge ROS. This study sought to evaluate the ability of CNP to mitigate iron-induced oxidative stress and assess cell viability in the human ARPE-19 cell line in vitro. Cell viability was measured by an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and compared between experimental groups undergoing 48-hr exposure to a ferrous iron solution with and without 24-hr CNP pre-treatment. The CNP effect on ROS formation was evaluated additionally by H2DCFDA (2,7-dichlorodihydrofluorescein diacetate) fluorescent probe assay and superoxide dismutase assay. CNP demonstrated a three-fold increase in cell viability and a reduction in ROS generation. The results show a promising treatment modality for diseases causing oxidative damage in the eye.
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spelling pubmed-74859922020-09-12 Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium Kieffer, Jason Singh, Sushant Dhillon, Baltej S Kumar, Udit Shaikh, Saad Ho, Son Seal, Sudipta Cureus Ophthalmology Oxidative injury is implicated in retinal damage observed in age-related macular degeneration (AMD), as well as other degenerative conditions. Abnormally elevated levels of iron accumulation within the retinal pigment epithelium have been detected in eyes with AMD, and it is suspected to play a role in the pathogenesis through the production of reactive oxygen species (ROS). Ceria nanoparticles (CNP) have the ability to scavenge ROS. This study sought to evaluate the ability of CNP to mitigate iron-induced oxidative stress and assess cell viability in the human ARPE-19 cell line in vitro. Cell viability was measured by an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and compared between experimental groups undergoing 48-hr exposure to a ferrous iron solution with and without 24-hr CNP pre-treatment. The CNP effect on ROS formation was evaluated additionally by H2DCFDA (2,7-dichlorodihydrofluorescein diacetate) fluorescent probe assay and superoxide dismutase assay. CNP demonstrated a three-fold increase in cell viability and a reduction in ROS generation. The results show a promising treatment modality for diseases causing oxidative damage in the eye. Cureus 2020-08-11 /pmc/articles/PMC7485992/ /pubmed/32923270 http://dx.doi.org/10.7759/cureus.9675 Text en Copyright © 2020, Kieffer et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Ophthalmology
Kieffer, Jason
Singh, Sushant
Dhillon, Baltej S
Kumar, Udit
Shaikh, Saad
Ho, Son
Seal, Sudipta
Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium
title Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium
title_full Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium
title_fullStr Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium
title_full_unstemmed Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium
title_short Ceria Nanoparticles Mitigate the Iron Oxidative Toxicity of Human Retinal Pigment Epithelium
title_sort ceria nanoparticles mitigate the iron oxidative toxicity of human retinal pigment epithelium
topic Ophthalmology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485992/
https://www.ncbi.nlm.nih.gov/pubmed/32923270
http://dx.doi.org/10.7759/cureus.9675
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