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Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats
BACKGROUND/PURPOSE: Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Association for Dental Sciences of the Republic of China
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486518/ https://www.ncbi.nlm.nih.gov/pubmed/32952894 http://dx.doi.org/10.1016/j.jds.2020.02.005 |
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author | Xu, Jie Zhao, Xian Zeng, Jin Yu, Jing-Hong Guan, Simon Xu, Xiao-Mei Mei, Li |
author_facet | Xu, Jie Zhao, Xian Zeng, Jin Yu, Jing-Hong Guan, Simon Xu, Xiao-Mei Mei, Li |
author_sort | Xu, Jie |
collection | PubMed |
description | BACKGROUND/PURPOSE: Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(TNF-α) expression in the periodontal ligaments (PDLs) of Sprague–Dawley (SD) rats to analyze the involvement of autophagy and inflammatory cytokines in orthodontic tooth movement (OTM) and maintaining periodontal tissue homeostasis. MATERIALS AND METHODS: SD rats (n = 100) were randomly divided into a control group (n = 10) and an experimental group (n = 90). An orthodontic appliance was placed in each rat in the experimental group, and 10 rats were randomly euthanized 15 min, 30 min, 1 h, 2 h, 4 h, 12 h, 1 d, 3 d and 7 d after mechanical loading. The OTM distance was then measured. Hematoxylin and eosin (HE) staining was used to analyze the morphology of the PDL. Immunohistochemical (IHC) staining and tartrate-resistant acid phosphatase (TRAP) staining were also performed. RESULTS: After the application of orthodontic force and under the dual effects of mechanical force and starvation caused by compressed vessels, the level of autophagy and TNF-α expression in the PDL fluctuated and exhibited a similar trend. CONCLUSION: Our data suggest a significant correlation between the initiation of autophagy and TNF-α expression, which both exerted positive effects on PDL remodeling during OTM in rats. |
format | Online Article Text |
id | pubmed-7486518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Association for Dental Sciences of the Republic of China |
record_format | MEDLINE/PubMed |
spelling | pubmed-74865182020-09-18 Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats Xu, Jie Zhao, Xian Zeng, Jin Yu, Jing-Hong Guan, Simon Xu, Xiao-Mei Mei, Li J Dent Sci Original Article BACKGROUND/PURPOSE: Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(TNF-α) expression in the periodontal ligaments (PDLs) of Sprague–Dawley (SD) rats to analyze the involvement of autophagy and inflammatory cytokines in orthodontic tooth movement (OTM) and maintaining periodontal tissue homeostasis. MATERIALS AND METHODS: SD rats (n = 100) were randomly divided into a control group (n = 10) and an experimental group (n = 90). An orthodontic appliance was placed in each rat in the experimental group, and 10 rats were randomly euthanized 15 min, 30 min, 1 h, 2 h, 4 h, 12 h, 1 d, 3 d and 7 d after mechanical loading. The OTM distance was then measured. Hematoxylin and eosin (HE) staining was used to analyze the morphology of the PDL. Immunohistochemical (IHC) staining and tartrate-resistant acid phosphatase (TRAP) staining were also performed. RESULTS: After the application of orthodontic force and under the dual effects of mechanical force and starvation caused by compressed vessels, the level of autophagy and TNF-α expression in the PDL fluctuated and exhibited a similar trend. CONCLUSION: Our data suggest a significant correlation between the initiation of autophagy and TNF-α expression, which both exerted positive effects on PDL remodeling during OTM in rats. Association for Dental Sciences of the Republic of China 2020-09 2020-03-24 /pmc/articles/PMC7486518/ /pubmed/32952894 http://dx.doi.org/10.1016/j.jds.2020.02.005 Text en © 2020 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Xu, Jie Zhao, Xian Zeng, Jin Yu, Jing-Hong Guan, Simon Xu, Xiao-Mei Mei, Li Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
title | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
title_full | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
title_fullStr | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
title_full_unstemmed | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
title_short | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
title_sort | role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486518/ https://www.ncbi.nlm.nih.gov/pubmed/32952894 http://dx.doi.org/10.1016/j.jds.2020.02.005 |
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