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Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma

BACKGROUND: The spondin-2 (SPON2) gene is overexpressed in multiple malignant tumors and may promote tumor aggressiveness. However, its expression profile and functional roles in clear cell renal cell carcinoma (ccRCC) are still unclear. METHODS: SPON2 expression in ccRCC was evaluated using express...

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Autores principales: Ma, Hui-Min, Yu, Meng, Wu, Cong, Huang, Hou-Bao, Li, Ya-Wei, Zhang, Peng, Huang, Jian-Jun, Cheng, Long, Feng, Gang, Li, Guo-Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487092/
https://www.ncbi.nlm.nih.gov/pubmed/32952742
http://dx.doi.org/10.1155/2020/5074239
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author Ma, Hui-Min
Yu, Meng
Wu, Cong
Huang, Hou-Bao
Li, Ya-Wei
Zhang, Peng
Huang, Jian-Jun
Cheng, Long
Feng, Gang
Li, Guo-Rong
author_facet Ma, Hui-Min
Yu, Meng
Wu, Cong
Huang, Hou-Bao
Li, Ya-Wei
Zhang, Peng
Huang, Jian-Jun
Cheng, Long
Feng, Gang
Li, Guo-Rong
author_sort Ma, Hui-Min
collection PubMed
description BACKGROUND: The spondin-2 (SPON2) gene is overexpressed in multiple malignant tumors and may promote tumor aggressiveness. However, its expression profile and functional roles in clear cell renal cell carcinoma (ccRCC) are still unclear. METHODS: SPON2 expression in ccRCC was evaluated using expression data from TCGA and GEO databases, then confirmed by local patient population (94 patients). The clinical significance of SPON2 expression was evaluated. Downregulation of SPON2 was performed using small-interfering RNA (siRNA). The effects of SPON2 silencing on cell proliferation, apoptosis, invasion, and migration in vitro were investigated. RESULTS: SPON2 was overexpressed in the majority of the ccRCC at both mRNA and protein levels. SPON2 expression was significantly correlated with stage, grade, and recurrence (all P < 0.05) in patients with localized ccRCC. The receiver operating characteristic (ROC) curve showed that SPON2 expression could serve as a predictor of recurrence. SPON2 expression was significantly associated with recurrence-free survival (RFS) in patients with localized ccRCC. Knocking down SPON2 resulted in suppressed cell invasion and migration in vitro. CONCLUSION: SPON2 expression might function as a prognostic biomarker in patients with localized ccRCC.
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spelling pubmed-74870922020-09-17 Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma Ma, Hui-Min Yu, Meng Wu, Cong Huang, Hou-Bao Li, Ya-Wei Zhang, Peng Huang, Jian-Jun Cheng, Long Feng, Gang Li, Guo-Rong Dis Markers Research Article BACKGROUND: The spondin-2 (SPON2) gene is overexpressed in multiple malignant tumors and may promote tumor aggressiveness. However, its expression profile and functional roles in clear cell renal cell carcinoma (ccRCC) are still unclear. METHODS: SPON2 expression in ccRCC was evaluated using expression data from TCGA and GEO databases, then confirmed by local patient population (94 patients). The clinical significance of SPON2 expression was evaluated. Downregulation of SPON2 was performed using small-interfering RNA (siRNA). The effects of SPON2 silencing on cell proliferation, apoptosis, invasion, and migration in vitro were investigated. RESULTS: SPON2 was overexpressed in the majority of the ccRCC at both mRNA and protein levels. SPON2 expression was significantly correlated with stage, grade, and recurrence (all P < 0.05) in patients with localized ccRCC. The receiver operating characteristic (ROC) curve showed that SPON2 expression could serve as a predictor of recurrence. SPON2 expression was significantly associated with recurrence-free survival (RFS) in patients with localized ccRCC. Knocking down SPON2 resulted in suppressed cell invasion and migration in vitro. CONCLUSION: SPON2 expression might function as a prognostic biomarker in patients with localized ccRCC. Hindawi 2020-09-03 /pmc/articles/PMC7487092/ /pubmed/32952742 http://dx.doi.org/10.1155/2020/5074239 Text en Copyright © 2020 Hui-Min Ma et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ma, Hui-Min
Yu, Meng
Wu, Cong
Huang, Hou-Bao
Li, Ya-Wei
Zhang, Peng
Huang, Jian-Jun
Cheng, Long
Feng, Gang
Li, Guo-Rong
Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma
title Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma
title_full Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma
title_fullStr Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma
title_full_unstemmed Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma
title_short Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma
title_sort overexpression of spondin-2 is associated with recurrence-free survival in patients with localized clear cell renal cell carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487092/
https://www.ncbi.nlm.nih.gov/pubmed/32952742
http://dx.doi.org/10.1155/2020/5074239
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