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Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica

BACKGROUND: Hepatic granuloma formation and fibrosis as the consequence of tissue entrapped eggs produced by female schistosomes characterize the pathology of Schistosoma japonicum infection. It has been proposed that fucoidan, a sulfated polysaccharide existing naturally in brown seaweed Fucus vesi...

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Autores principales: Bai, Xueqi, Li, Maining, Wang, Xinyue, Chang, Hao, Ni, Yangyue, Li, Chen, He, Kaiyue, Wang, Huiquan, Yang, Yuxuan, Tian, Tian, Hou, Min, Ji, Minjun, Xu, Zhipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487607/
https://www.ncbi.nlm.nih.gov/pubmed/32894174
http://dx.doi.org/10.1186/s13071-020-04332-7
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author Bai, Xueqi
Li, Maining
Wang, Xinyue
Chang, Hao
Ni, Yangyue
Li, Chen
He, Kaiyue
Wang, Huiquan
Yang, Yuxuan
Tian, Tian
Hou, Min
Ji, Minjun
Xu, Zhipeng
author_facet Bai, Xueqi
Li, Maining
Wang, Xinyue
Chang, Hao
Ni, Yangyue
Li, Chen
He, Kaiyue
Wang, Huiquan
Yang, Yuxuan
Tian, Tian
Hou, Min
Ji, Minjun
Xu, Zhipeng
author_sort Bai, Xueqi
collection PubMed
description BACKGROUND: Hepatic granuloma formation and fibrosis as the consequence of tissue entrapped eggs produced by female schistosomes characterize the pathology of Schistosoma japonicum infection. It has been proposed that fucoidan, a sulfated polysaccharide existing naturally in brown seaweed Fucus vesiculosus, plays a diversified role to perform immunomodulatory activities. However, whether fucoidan functions in the host hepatic pathology is unknown and identifying the potential mechanism that is responsible for hepatic improvement is still necessary. METHODS: We evaluated the hepatic pathology from S. japonicum-infected mice after treatment with fucoidan. qRT-PCR and immunofluorescence were used to detect the pro- or anti-inflammatory factors and the phosphorylated p65 in the livers. In addition, flow cytometry was also performed to investigate the T cell subsets in the S. japonicum-infected mice after treatment with fucoidan, and functional molecules relatively specific to Treg cells were detected in vitro. Furthermore, macrophages were treated with fucoidan in vitro and to detect the inflammatory cytokines. RESULTS: Treatment with fucoidan significantly reduced the hepatic granuloma size and fibrosis response during S. japonicum infection. The attenuated phospho-p65 protein levels and the mRNA levels of pro-inflammatory cytokines (IL-6, IL-12 and TNF-α) were observed in the livers from fucoidan-treated S. japonicum-infected mice; however, the mRNA levels of anti-inflammatory cytokines (IL-4 and IL-13) were increased. In addition, the infiltration of Treg cells was significantly enhanced both in the livers and spleens from fucoidan-treated S. japonicum-infected mice. Consistent with this, the mRNA levels of IL-10 and TGF-β were dramatically increased in the livers from S. japonicum-infected mice after fucoidan treatment. Furthermore, in vitro stimulated splenocytes with fucoidan resulted in increasing Treg cells in splenocytes as well as the functional expression of CC chemokine receptor type 4 (CCR4) and CXC chemokine receptor type 5 (CXCR5) in Treg cells. Additionally, fucoidan promoted the mRNA levels of IL-4 and IL-13 in macrophages. CONCLUSIONS: These findings suggest an important role of natural fucoidan in reducing hepatic pathology in the progress of S. japonicum infection with a stronger Treg response, which may reveal a new potential therapeutic strategy for hepatic disease caused by parasitic chronic infection. [Image: see text]
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spelling pubmed-74876072020-09-15 Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica Bai, Xueqi Li, Maining Wang, Xinyue Chang, Hao Ni, Yangyue Li, Chen He, Kaiyue Wang, Huiquan Yang, Yuxuan Tian, Tian Hou, Min Ji, Minjun Xu, Zhipeng Parasit Vectors Research BACKGROUND: Hepatic granuloma formation and fibrosis as the consequence of tissue entrapped eggs produced by female schistosomes characterize the pathology of Schistosoma japonicum infection. It has been proposed that fucoidan, a sulfated polysaccharide existing naturally in brown seaweed Fucus vesiculosus, plays a diversified role to perform immunomodulatory activities. However, whether fucoidan functions in the host hepatic pathology is unknown and identifying the potential mechanism that is responsible for hepatic improvement is still necessary. METHODS: We evaluated the hepatic pathology from S. japonicum-infected mice after treatment with fucoidan. qRT-PCR and immunofluorescence were used to detect the pro- or anti-inflammatory factors and the phosphorylated p65 in the livers. In addition, flow cytometry was also performed to investigate the T cell subsets in the S. japonicum-infected mice after treatment with fucoidan, and functional molecules relatively specific to Treg cells were detected in vitro. Furthermore, macrophages were treated with fucoidan in vitro and to detect the inflammatory cytokines. RESULTS: Treatment with fucoidan significantly reduced the hepatic granuloma size and fibrosis response during S. japonicum infection. The attenuated phospho-p65 protein levels and the mRNA levels of pro-inflammatory cytokines (IL-6, IL-12 and TNF-α) were observed in the livers from fucoidan-treated S. japonicum-infected mice; however, the mRNA levels of anti-inflammatory cytokines (IL-4 and IL-13) were increased. In addition, the infiltration of Treg cells was significantly enhanced both in the livers and spleens from fucoidan-treated S. japonicum-infected mice. Consistent with this, the mRNA levels of IL-10 and TGF-β were dramatically increased in the livers from S. japonicum-infected mice after fucoidan treatment. Furthermore, in vitro stimulated splenocytes with fucoidan resulted in increasing Treg cells in splenocytes as well as the functional expression of CC chemokine receptor type 4 (CCR4) and CXC chemokine receptor type 5 (CXCR5) in Treg cells. Additionally, fucoidan promoted the mRNA levels of IL-4 and IL-13 in macrophages. CONCLUSIONS: These findings suggest an important role of natural fucoidan in reducing hepatic pathology in the progress of S. japonicum infection with a stronger Treg response, which may reveal a new potential therapeutic strategy for hepatic disease caused by parasitic chronic infection. [Image: see text] BioMed Central 2020-09-07 /pmc/articles/PMC7487607/ /pubmed/32894174 http://dx.doi.org/10.1186/s13071-020-04332-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bai, Xueqi
Li, Maining
Wang, Xinyue
Chang, Hao
Ni, Yangyue
Li, Chen
He, Kaiyue
Wang, Huiquan
Yang, Yuxuan
Tian, Tian
Hou, Min
Ji, Minjun
Xu, Zhipeng
Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
title Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
title_full Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
title_fullStr Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
title_full_unstemmed Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
title_short Therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
title_sort therapeutic potential of fucoidan in the reduction of hepatic pathology in murine schistosomiasis japonica
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487607/
https://www.ncbi.nlm.nih.gov/pubmed/32894174
http://dx.doi.org/10.1186/s13071-020-04332-7
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