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Immunohistochemical basigin expression level in thyroid cancer tissues
BACKGROUND: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. METHODS: The BS...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487720/ https://www.ncbi.nlm.nih.gov/pubmed/32891152 http://dx.doi.org/10.1186/s12957-020-01975-9 |
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author | Guo, Wan-Ping Tang, Deng Pang, Yu-Yan Li, Xiao-Jiao Chen, Gang Huang, Zhi-Guang Tang, Xiao-Zhun Lai, Qin-Qiao Gan, Jin-Yan Huang, Xiao-Li Liu, Xiao-Fan Wei, Zhi-Xiao Ma, Wei |
author_facet | Guo, Wan-Ping Tang, Deng Pang, Yu-Yan Li, Xiao-Jiao Chen, Gang Huang, Zhi-Guang Tang, Xiao-Zhun Lai, Qin-Qiao Gan, Jin-Yan Huang, Xiao-Li Liu, Xiao-Fan Wei, Zhi-Xiao Ma, Wei |
author_sort | Guo, Wan-Ping |
collection | PubMed |
description | BACKGROUND: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. METHODS: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays, and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. RESULTS: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly upregulated in TC cases. CONCLUSION: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions. |
format | Online Article Text |
id | pubmed-7487720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74877202020-09-16 Immunohistochemical basigin expression level in thyroid cancer tissues Guo, Wan-Ping Tang, Deng Pang, Yu-Yan Li, Xiao-Jiao Chen, Gang Huang, Zhi-Guang Tang, Xiao-Zhun Lai, Qin-Qiao Gan, Jin-Yan Huang, Xiao-Li Liu, Xiao-Fan Wei, Zhi-Xiao Ma, Wei World J Surg Oncol Research BACKGROUND: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. METHODS: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays, and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. RESULTS: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly upregulated in TC cases. CONCLUSION: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions. BioMed Central 2020-09-05 /pmc/articles/PMC7487720/ /pubmed/32891152 http://dx.doi.org/10.1186/s12957-020-01975-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Guo, Wan-Ping Tang, Deng Pang, Yu-Yan Li, Xiao-Jiao Chen, Gang Huang, Zhi-Guang Tang, Xiao-Zhun Lai, Qin-Qiao Gan, Jin-Yan Huang, Xiao-Li Liu, Xiao-Fan Wei, Zhi-Xiao Ma, Wei Immunohistochemical basigin expression level in thyroid cancer tissues |
title | Immunohistochemical basigin expression level in thyroid cancer tissues |
title_full | Immunohistochemical basigin expression level in thyroid cancer tissues |
title_fullStr | Immunohistochemical basigin expression level in thyroid cancer tissues |
title_full_unstemmed | Immunohistochemical basigin expression level in thyroid cancer tissues |
title_short | Immunohistochemical basigin expression level in thyroid cancer tissues |
title_sort | immunohistochemical basigin expression level in thyroid cancer tissues |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487720/ https://www.ncbi.nlm.nih.gov/pubmed/32891152 http://dx.doi.org/10.1186/s12957-020-01975-9 |
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