Cargando…
Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research
Embryonic stem cells (ESCs) derived from somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) are promising tools for meeting the personalized requirements of regenerative medicine. However, some obstacles need to be overcome before clinical trials can be undertaken. First...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487873/ https://www.ncbi.nlm.nih.gov/pubmed/32894201 http://dx.doi.org/10.1186/s13287-020-01907-0 |
_version_ | 1783581578045161472 |
---|---|
author | Zhang, Zhi-ping Zhang, Jun-tao Huang, Shu-cheng He, Xiu-yuan Deng, Li-xin |
author_facet | Zhang, Zhi-ping Zhang, Jun-tao Huang, Shu-cheng He, Xiu-yuan Deng, Li-xin |
author_sort | Zhang, Zhi-ping |
collection | PubMed |
description | Embryonic stem cells (ESCs) derived from somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) are promising tools for meeting the personalized requirements of regenerative medicine. However, some obstacles need to be overcome before clinical trials can be undertaken. First, donor cells vary, and the reprogramming procedures are diverse, so standardization is a great obstacle regarding SCNT and iPSCs. Second, somatic cells derived from a patient may carry mitochondrial DNA mutations and exhibit telomere instability with aging or disease, and SCNT-ESCs and iPSCs retain the epigenetic memory or epigenetic modification errors. Third, reprogramming efficiency has remained low. Therefore, in addition to improving their success rate, other alternatives for producing ESCs should be explored. Producing androgenetic diploid embryos could be an outstanding strategy; androgenic diploid embryos are produced through double sperm cloning (DSC), in which two capacitated sperms (XY or XX, sorted by flow cytometer) are injected into a denucleated oocyte by intracytoplasmic sperm injection (ICSI) to reconstruct embryo and derive DSC-ESCs. This process could avoid some potential issues, such as mitochondrial interference, telomere shortening, and somatic epigenetic memory, all of which accompany somatic donor cells. Oocytes are naturally activated by sperm, which is unlike the artificial activation that occurs in SCNT. The procedure is simple and practical and can be easily standardized. In addition, DSC-ESCs can overcome ethical concerns and resolve immunological response matching with sperm providers. Certainly, some challenges must be faced regarding imprinted genes, epigenetics, X chromosome inactivation, and dosage compensation. In mice, DSC-ESCs have been produced and have shown excellent differentiation ability. Therefore, the many advantages of DSC make the study of this process worthwhile for regenerative medicine and animal breeding. |
format | Online Article Text |
id | pubmed-7487873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74878732020-09-16 Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research Zhang, Zhi-ping Zhang, Jun-tao Huang, Shu-cheng He, Xiu-yuan Deng, Li-xin Stem Cell Res Ther Review Embryonic stem cells (ESCs) derived from somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) are promising tools for meeting the personalized requirements of regenerative medicine. However, some obstacles need to be overcome before clinical trials can be undertaken. First, donor cells vary, and the reprogramming procedures are diverse, so standardization is a great obstacle regarding SCNT and iPSCs. Second, somatic cells derived from a patient may carry mitochondrial DNA mutations and exhibit telomere instability with aging or disease, and SCNT-ESCs and iPSCs retain the epigenetic memory or epigenetic modification errors. Third, reprogramming efficiency has remained low. Therefore, in addition to improving their success rate, other alternatives for producing ESCs should be explored. Producing androgenetic diploid embryos could be an outstanding strategy; androgenic diploid embryos are produced through double sperm cloning (DSC), in which two capacitated sperms (XY or XX, sorted by flow cytometer) are injected into a denucleated oocyte by intracytoplasmic sperm injection (ICSI) to reconstruct embryo and derive DSC-ESCs. This process could avoid some potential issues, such as mitochondrial interference, telomere shortening, and somatic epigenetic memory, all of which accompany somatic donor cells. Oocytes are naturally activated by sperm, which is unlike the artificial activation that occurs in SCNT. The procedure is simple and practical and can be easily standardized. In addition, DSC-ESCs can overcome ethical concerns and resolve immunological response matching with sperm providers. Certainly, some challenges must be faced regarding imprinted genes, epigenetics, X chromosome inactivation, and dosage compensation. In mice, DSC-ESCs have been produced and have shown excellent differentiation ability. Therefore, the many advantages of DSC make the study of this process worthwhile for regenerative medicine and animal breeding. BioMed Central 2020-09-07 /pmc/articles/PMC7487873/ /pubmed/32894201 http://dx.doi.org/10.1186/s13287-020-01907-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Review Zhang, Zhi-ping Zhang, Jun-tao Huang, Shu-cheng He, Xiu-yuan Deng, Li-xin Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research |
title | Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research |
title_full | Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research |
title_fullStr | Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research |
title_full_unstemmed | Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research |
title_short | Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research |
title_sort | double sperm cloning (dsc) is a promising strategy in mammalian genetic engineering and stem cell research |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487873/ https://www.ncbi.nlm.nih.gov/pubmed/32894201 http://dx.doi.org/10.1186/s13287-020-01907-0 |
work_keys_str_mv | AT zhangzhiping doublespermcloningdscisapromisingstrategyinmammaliangeneticengineeringandstemcellresearch AT zhangjuntao doublespermcloningdscisapromisingstrategyinmammaliangeneticengineeringandstemcellresearch AT huangshucheng doublespermcloningdscisapromisingstrategyinmammaliangeneticengineeringandstemcellresearch AT hexiuyuan doublespermcloningdscisapromisingstrategyinmammaliangeneticengineeringandstemcellresearch AT denglixin doublespermcloningdscisapromisingstrategyinmammaliangeneticengineeringandstemcellresearch |