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Establishment and characterization of a new gastric cancer cell line, XGC-1

BACKGROUND: To establish a primary human gastric cancer cell line. METHODS: Fresh gastric cancer tissue samples were separated into a cell suspension, and DMEM/F12 medium containing 10% foetal bovine serum was used for primary culture and subculture. The morphology of the cells was observed under a...

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Detalles Bibliográficos
Autores principales: Peng, Jigui, Xu, Hao, Cai, Jianchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487967/
https://www.ncbi.nlm.nih.gov/pubmed/32943986
http://dx.doi.org/10.1186/s12935-020-01536-w
Descripción
Sumario:BACKGROUND: To establish a primary human gastric cancer cell line. METHODS: Fresh gastric cancer tissue samples were separated into a cell suspension, and DMEM/F12 medium containing 10% foetal bovine serum was used for primary culture and subculture. The morphology of the cells was observed under a light microscope, and the cell growth curve was plotted. A soft agar colony formation assay was used to detect the colony formation ability of the cell line. Immunohistochemical methods were used to detect cytokeratin, vimentin and Ki-67, the chromosome G banding method was used to analyse the karyotype of the cells, and the tumourigenic ability of the cells was detected by subcutaneous inoculation of BALB/C nude mice. RESULTS: We established a gastric cancer cell line from a 68-year-old male patient. This gastric cancer cell line was named XGC-1 and had a doubling time of approximately 48 h. The cell line displayed strong colony formation ability and tumourigenicity in BALB/C nude mice and had complicated chromosomal abnormalities. When nutrients were insufficient, the cells shed and floated in the medium, but adherent growth was observed in nutrient-rich conditions. CONCLUSIONS: The XGC-1 cell line will be useful for future studies of gastric cancer development, progression, metastasis and therapy.