Cargando…

Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking

PURPOSE: Genipin has been proposed as a possible neuroprotective therapy in myopia and glaucoma. Here, we aim to determine the effects of prolonged genipin-induced scleral stiffening on visual function. METHODS: Eyes from Brown Norway rats were treated in vivo with either a single 15 mM genipin retr...

Descripción completa

Detalles Bibliográficos
Autores principales: Hannon, Bailey G., Luna, Coralia, Feola, Andrew J., Ritch, Matthew D., Read, A. Thomas, Stinnett, Sandra S., Vo, Harrison, Pardue, Machelle T., Gonzalez, Pedro, Ethier, C. Ross
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488211/
https://www.ncbi.nlm.nih.gov/pubmed/32974080
http://dx.doi.org/10.1167/tvst.9.10.8
_version_ 1783581647085502464
author Hannon, Bailey G.
Luna, Coralia
Feola, Andrew J.
Ritch, Matthew D.
Read, A. Thomas
Stinnett, Sandra S.
Vo, Harrison
Pardue, Machelle T.
Gonzalez, Pedro
Ethier, C. Ross
author_facet Hannon, Bailey G.
Luna, Coralia
Feola, Andrew J.
Ritch, Matthew D.
Read, A. Thomas
Stinnett, Sandra S.
Vo, Harrison
Pardue, Machelle T.
Gonzalez, Pedro
Ethier, C. Ross
author_sort Hannon, Bailey G.
collection PubMed
description PURPOSE: Genipin has been proposed as a possible neuroprotective therapy in myopia and glaucoma. Here, we aim to determine the effects of prolonged genipin-induced scleral stiffening on visual function. METHODS: Eyes from Brown Norway rats were treated in vivo with either a single 15 mM genipin retrobulbar injection or sham retrobulbar injection and were compared to naïve eyes. Intraocular pressure, optomotor response, and electroretinograms were repeatedly measured over 4 weeks following retrobulbar injections to determine visual and retinal function. At 4 weeks, we quantified retinal ganglion cell axon counts. Finally, molecular changes in gene and protein expression were analyzed via real-time polymerase chain reaction (RT-PCR) and proteomics. RESULTS: Retrobulbar injection of genipin did not affect intraocular pressure (IOP) or retinal function, nor have a sustained impact on visual function. Although genipin-treated eyes had a small decrease in retinal ganglion cell axon counts compared to contralateral sham-treated eyes (−8,558 ± 18,646; mean ± SD), this was not statistically significant (P = 0.206, n = 9). Last, we did not observe any changes in gene or protein expression due to genipin treatment. CONCLUSIONS: Posterior scleral stiffening with a single retrobulbar injection of 15 mM genipin causes no sustained deficits in visual or retinal function or at the molecular level in the retina and sclera. Retinal ganglion cell axon morphology appeared normal. TRANSLATIONAL SIGNIFICANCE: These results support future in vivo studies to determine the efficacy of genipin-induced posterior scleral stiffening to help treat ocular diseases, like myopia and glaucoma.
format Online
Article
Text
id pubmed-7488211
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Association for Research in Vision and Ophthalmology
record_format MEDLINE/PubMed
spelling pubmed-74882112020-09-23 Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking Hannon, Bailey G. Luna, Coralia Feola, Andrew J. Ritch, Matthew D. Read, A. Thomas Stinnett, Sandra S. Vo, Harrison Pardue, Machelle T. Gonzalez, Pedro Ethier, C. Ross Transl Vis Sci Technol Article PURPOSE: Genipin has been proposed as a possible neuroprotective therapy in myopia and glaucoma. Here, we aim to determine the effects of prolonged genipin-induced scleral stiffening on visual function. METHODS: Eyes from Brown Norway rats were treated in vivo with either a single 15 mM genipin retrobulbar injection or sham retrobulbar injection and were compared to naïve eyes. Intraocular pressure, optomotor response, and electroretinograms were repeatedly measured over 4 weeks following retrobulbar injections to determine visual and retinal function. At 4 weeks, we quantified retinal ganglion cell axon counts. Finally, molecular changes in gene and protein expression were analyzed via real-time polymerase chain reaction (RT-PCR) and proteomics. RESULTS: Retrobulbar injection of genipin did not affect intraocular pressure (IOP) or retinal function, nor have a sustained impact on visual function. Although genipin-treated eyes had a small decrease in retinal ganglion cell axon counts compared to contralateral sham-treated eyes (−8,558 ± 18,646; mean ± SD), this was not statistically significant (P = 0.206, n = 9). Last, we did not observe any changes in gene or protein expression due to genipin treatment. CONCLUSIONS: Posterior scleral stiffening with a single retrobulbar injection of 15 mM genipin causes no sustained deficits in visual or retinal function or at the molecular level in the retina and sclera. Retinal ganglion cell axon morphology appeared normal. TRANSLATIONAL SIGNIFICANCE: These results support future in vivo studies to determine the efficacy of genipin-induced posterior scleral stiffening to help treat ocular diseases, like myopia and glaucoma. The Association for Research in Vision and Ophthalmology 2020-09-08 /pmc/articles/PMC7488211/ /pubmed/32974080 http://dx.doi.org/10.1167/tvst.9.10.8 Text en Copyright 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Article
Hannon, Bailey G.
Luna, Coralia
Feola, Andrew J.
Ritch, Matthew D.
Read, A. Thomas
Stinnett, Sandra S.
Vo, Harrison
Pardue, Machelle T.
Gonzalez, Pedro
Ethier, C. Ross
Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking
title Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking
title_full Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking
title_fullStr Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking
title_full_unstemmed Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking
title_short Assessment of Visual and Retinal Function Following In Vivo Genipin-Induced Scleral Crosslinking
title_sort assessment of visual and retinal function following in vivo genipin-induced scleral crosslinking
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488211/
https://www.ncbi.nlm.nih.gov/pubmed/32974080
http://dx.doi.org/10.1167/tvst.9.10.8
work_keys_str_mv AT hannonbaileyg assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT lunacoralia assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT feolaandrewj assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT ritchmatthewd assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT readathomas assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT stinnettsandras assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT voharrison assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT parduemachellet assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT gonzalezpedro assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking
AT ethiercross assessmentofvisualandretinalfunctionfollowinginvivogenipininducedscleralcrosslinking