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Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases
BACKGROUND: Frequencies of polymicrobial infection and pathogens evidenced in course of infected nonunion treatment are largely unknown. Therefore, this study aims at investigating microbial patterns in infected nonunions. METHODS: Surgically treated patients with long bone infected nonunion admitte...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488339/ https://www.ncbi.nlm.nih.gov/pubmed/32912204 http://dx.doi.org/10.1186/s12879-020-05386-9 |
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author | Rupp, Markus Kern, Stefanie Weber, Tobias Menges, Tamina D. Schnettler, Reinhard Heiß, Christian Alt, Volker |
author_facet | Rupp, Markus Kern, Stefanie Weber, Tobias Menges, Tamina D. Schnettler, Reinhard Heiß, Christian Alt, Volker |
author_sort | Rupp, Markus |
collection | PubMed |
description | BACKGROUND: Frequencies of polymicrobial infection and pathogens evidenced in course of infected nonunion treatment are largely unknown. Therefore, this study aims at investigating microbial patterns in infected nonunions. METHODS: Surgically treated patients with long bone infected nonunion admitted between January 2010 and March 2018 were included in the study. Microbiological culture and polymerase-chain-reaction results of tissue samples of initial and follow-up revision surgeries were assessed and compared with patient and treatment characteristics. RESULTS: Forty two patients with a mean age of 53.9 ± 17.7 years were included. In six patients (14.3%) polymicrobial infection was evident. A change of pathogens evidenced in course of the treatment occurred in 21 patients (50%). In 16 patients (38.1%) previously detected bacteria could be determined by microbial testing after further revision surgery. Staphylococcus aureus was most often detected (n = 34, 30.6%), followed by Enterococcus spp. (n = 25, 22.5%) and Staphylococcus epidermidis (n = 18, 16.2%). Five Staphylococcus aureus were resistant to methicillin (MRSA). In patients without polymicrobial infection or further germ detection in course of the treatment, 86.4% of the infections were due to Staphylococcus spp.. Infections due to Streptococcus spp. and gram-negative bacteria were only present in patients with polymicrobial infection and germ-change in course of the treatment. CONCLUSION: A low rate of polymicrobial infections was evidenced in the present study. Germ-change often occurs in course of revision surgeries. Prospective studies with more sensitive diagnostic tools are necessary to elucidate the therapeutical relevance of microbiological testing results for surgical as well as medical treatment in infected nonunions. |
format | Online Article Text |
id | pubmed-7488339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74883392020-09-16 Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases Rupp, Markus Kern, Stefanie Weber, Tobias Menges, Tamina D. Schnettler, Reinhard Heiß, Christian Alt, Volker BMC Infect Dis Research Article BACKGROUND: Frequencies of polymicrobial infection and pathogens evidenced in course of infected nonunion treatment are largely unknown. Therefore, this study aims at investigating microbial patterns in infected nonunions. METHODS: Surgically treated patients with long bone infected nonunion admitted between January 2010 and March 2018 were included in the study. Microbiological culture and polymerase-chain-reaction results of tissue samples of initial and follow-up revision surgeries were assessed and compared with patient and treatment characteristics. RESULTS: Forty two patients with a mean age of 53.9 ± 17.7 years were included. In six patients (14.3%) polymicrobial infection was evident. A change of pathogens evidenced in course of the treatment occurred in 21 patients (50%). In 16 patients (38.1%) previously detected bacteria could be determined by microbial testing after further revision surgery. Staphylococcus aureus was most often detected (n = 34, 30.6%), followed by Enterococcus spp. (n = 25, 22.5%) and Staphylococcus epidermidis (n = 18, 16.2%). Five Staphylococcus aureus were resistant to methicillin (MRSA). In patients without polymicrobial infection or further germ detection in course of the treatment, 86.4% of the infections were due to Staphylococcus spp.. Infections due to Streptococcus spp. and gram-negative bacteria were only present in patients with polymicrobial infection and germ-change in course of the treatment. CONCLUSION: A low rate of polymicrobial infections was evidenced in the present study. Germ-change often occurs in course of revision surgeries. Prospective studies with more sensitive diagnostic tools are necessary to elucidate the therapeutical relevance of microbiological testing results for surgical as well as medical treatment in infected nonunions. BioMed Central 2020-09-10 /pmc/articles/PMC7488339/ /pubmed/32912204 http://dx.doi.org/10.1186/s12879-020-05386-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Rupp, Markus Kern, Stefanie Weber, Tobias Menges, Tamina D. Schnettler, Reinhard Heiß, Christian Alt, Volker Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
title | Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
title_full | Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
title_fullStr | Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
title_full_unstemmed | Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
title_short | Polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
title_sort | polymicrobial infections and microbial patterns in infected nonunions – a descriptive analysis of 42 cases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488339/ https://www.ncbi.nlm.nih.gov/pubmed/32912204 http://dx.doi.org/10.1186/s12879-020-05386-9 |
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