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Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion

BACKGROUND: The quantification of nanomaterials accumulated in various organs is crucial in studying their toxicity and toxicokinetics. However, some types of nanomaterials, including carbon nanomaterials (CNMs), are difficult to quantify in a biological matrix. Therefore, developing improved method...

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Autores principales: Lee, Dong-Keun, Jeon, Soyeon, Jeong, Jiyoung, Song, Kyung Seuk, Cho, Wan-Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488454/
https://www.ncbi.nlm.nih.gov/pubmed/32917232
http://dx.doi.org/10.1186/s12989-020-00377-9
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author Lee, Dong-Keun
Jeon, Soyeon
Jeong, Jiyoung
Song, Kyung Seuk
Cho, Wan-Seob
author_facet Lee, Dong-Keun
Jeon, Soyeon
Jeong, Jiyoung
Song, Kyung Seuk
Cho, Wan-Seob
author_sort Lee, Dong-Keun
collection PubMed
description BACKGROUND: The quantification of nanomaterials accumulated in various organs is crucial in studying their toxicity and toxicokinetics. However, some types of nanomaterials, including carbon nanomaterials (CNMs), are difficult to quantify in a biological matrix. Therefore, developing improved methodologies for quantification of CNMs in vital organs is instrumental in their continued modification and application. RESULTS: In this study, carbon black, nanodiamond, multi-walled carbon nanotube, carbon nanofiber, and graphene nanoplatelet were assembled and used as a panel of CNMs. All CNMs showed significant absorbance at 750 nm, while their bio-components showed minimal absorbance at this wavelength. Quantification of CNMs using their absorbance at 750 nm was shown to have more than 94% accuracy in all of the studied materials. Incubating proteinase K (PK) for 2 days with a mixture of lung tissue homogenates and CNMs showed an average recovery rate over 90%. The utility of this method was confirmed in a murine pharyngeal aspiration model using CNMs at 30 μg/mouse. CONCLUSIONS: We developed an improved lung burden assay for CNMs with an accuracy > 94% and a recovery rate > 90% using PK digestion and UV-Vis spectrophotometry. This method can be applied to any nanomaterial with sufficient absorbance in the near-infrared band and can differentiate nanomaterials from elements in the body, as well as the soluble fraction of the nanomaterial. Furthermore, a combination of PK digestion and other instrumental analysis specific to the nanomaterial can be applied to organ burden analysis.
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spelling pubmed-74884542020-09-16 Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion Lee, Dong-Keun Jeon, Soyeon Jeong, Jiyoung Song, Kyung Seuk Cho, Wan-Seob Part Fibre Toxicol Research BACKGROUND: The quantification of nanomaterials accumulated in various organs is crucial in studying their toxicity and toxicokinetics. However, some types of nanomaterials, including carbon nanomaterials (CNMs), are difficult to quantify in a biological matrix. Therefore, developing improved methodologies for quantification of CNMs in vital organs is instrumental in their continued modification and application. RESULTS: In this study, carbon black, nanodiamond, multi-walled carbon nanotube, carbon nanofiber, and graphene nanoplatelet were assembled and used as a panel of CNMs. All CNMs showed significant absorbance at 750 nm, while their bio-components showed minimal absorbance at this wavelength. Quantification of CNMs using their absorbance at 750 nm was shown to have more than 94% accuracy in all of the studied materials. Incubating proteinase K (PK) for 2 days with a mixture of lung tissue homogenates and CNMs showed an average recovery rate over 90%. The utility of this method was confirmed in a murine pharyngeal aspiration model using CNMs at 30 μg/mouse. CONCLUSIONS: We developed an improved lung burden assay for CNMs with an accuracy > 94% and a recovery rate > 90% using PK digestion and UV-Vis spectrophotometry. This method can be applied to any nanomaterial with sufficient absorbance in the near-infrared band and can differentiate nanomaterials from elements in the body, as well as the soluble fraction of the nanomaterial. Furthermore, a combination of PK digestion and other instrumental analysis specific to the nanomaterial can be applied to organ burden analysis. BioMed Central 2020-09-11 /pmc/articles/PMC7488454/ /pubmed/32917232 http://dx.doi.org/10.1186/s12989-020-00377-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lee, Dong-Keun
Jeon, Soyeon
Jeong, Jiyoung
Song, Kyung Seuk
Cho, Wan-Seob
Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion
title Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion
title_full Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion
title_fullStr Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion
title_full_unstemmed Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion
title_short Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion
title_sort carbon nanomaterial-derived lung burden analysis using uv-vis spectrophotometry and proteinase k digestion
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488454/
https://www.ncbi.nlm.nih.gov/pubmed/32917232
http://dx.doi.org/10.1186/s12989-020-00377-9
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