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Does methylene blue reduce adhesion during the healing process after tendon repair?
OBJECTIVES: This study aims to biomechanically and histopathologically investigate the effects of methylene blue (MB) on preventing postoperative adhesion in chickens undergoing full- thickness flexor tendon incision. MATERIALS AND METHODS: This study was performed between June 2017 and June 2018 on...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bayçınar Medical Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489185/ https://www.ncbi.nlm.nih.gov/pubmed/32584721 http://dx.doi.org/10.5606/ehc.2020.74405 |
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author | Boz, Mehmet Çakıcı, Hüsamettin Pakdil, Murat Şahin, Abdullah Alper Erdoğan Düzcü, Selma Bala, Mehmet Murat Çelik, Metin |
author_facet | Boz, Mehmet Çakıcı, Hüsamettin Pakdil, Murat Şahin, Abdullah Alper Erdoğan Düzcü, Selma Bala, Mehmet Murat Çelik, Metin |
author_sort | Boz, Mehmet |
collection | PubMed |
description | OBJECTIVES: This study aims to biomechanically and histopathologically investigate the effects of methylene blue (MB) on preventing postoperative adhesion in chickens undergoing full- thickness flexor tendon incision. MATERIALS AND METHODS: This study was performed between June 2017 and June 2018 on Hubbard JA 57 type chickens (age, 6 months; weight, 2.2±0.3 kg). Sixty-four tendons were studied in 32 chickens, including 16 in the control group and 16 in the experimental group. The second and third finger flexor digitorum profundus tendons of the left foot of each chicken were repaired primarily after a full-thickness incision approximately 1 cm proximal to the distal adhesion area. In the control (n=32) and experimental groups (n=32), 0.25 mL of normal saline and 0.25 mL of 1% MB solutions were applied locally to the surgical site, respectively. The operated limb was immobilized using a circular cast. 16 chickens were randomly selected in each group and examined at the fourth week, and the remaining 16 chickens were examined at the sixth week. Thirty-two of these tendons were evaluated using the Tang histopathological adhesion classification system, and the other 32 were evaluated with a biomechanical pull system. RESULTS: Adhesion was found to be less in the experimental group compared to the control group at the end of the fourth and sixth weeks both histopathologically and biomechanically. Furthermore, adhesion was significantly less in the experimental group at the end of the sixth week compared to the fourth week both histopathologically and biomechanically. CONCLUSION: Histopathological and biomechanical results show that MB, which has anti-inflammatory, antiseptic, antimicrobial and antioxidant effects, reduces adhesion during the healing process after tendon repair. We think that local MB application, particularly in surgeries performed after this type of injury, will be beneficial on early rehabilitation and functional results. |
format | Online Article Text |
id | pubmed-7489185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Bayçınar Medical Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-74891852020-09-17 Does methylene blue reduce adhesion during the healing process after tendon repair? Boz, Mehmet Çakıcı, Hüsamettin Pakdil, Murat Şahin, Abdullah Alper Erdoğan Düzcü, Selma Bala, Mehmet Murat Çelik, Metin Jt Dis Relat Surg Original Article OBJECTIVES: This study aims to biomechanically and histopathologically investigate the effects of methylene blue (MB) on preventing postoperative adhesion in chickens undergoing full- thickness flexor tendon incision. MATERIALS AND METHODS: This study was performed between June 2017 and June 2018 on Hubbard JA 57 type chickens (age, 6 months; weight, 2.2±0.3 kg). Sixty-four tendons were studied in 32 chickens, including 16 in the control group and 16 in the experimental group. The second and third finger flexor digitorum profundus tendons of the left foot of each chicken were repaired primarily after a full-thickness incision approximately 1 cm proximal to the distal adhesion area. In the control (n=32) and experimental groups (n=32), 0.25 mL of normal saline and 0.25 mL of 1% MB solutions were applied locally to the surgical site, respectively. The operated limb was immobilized using a circular cast. 16 chickens were randomly selected in each group and examined at the fourth week, and the remaining 16 chickens were examined at the sixth week. Thirty-two of these tendons were evaluated using the Tang histopathological adhesion classification system, and the other 32 were evaluated with a biomechanical pull system. RESULTS: Adhesion was found to be less in the experimental group compared to the control group at the end of the fourth and sixth weeks both histopathologically and biomechanically. Furthermore, adhesion was significantly less in the experimental group at the end of the sixth week compared to the fourth week both histopathologically and biomechanically. CONCLUSION: Histopathological and biomechanical results show that MB, which has anti-inflammatory, antiseptic, antimicrobial and antioxidant effects, reduces adhesion during the healing process after tendon repair. We think that local MB application, particularly in surgeries performed after this type of injury, will be beneficial on early rehabilitation and functional results. Bayçınar Medical Publishing 2020-06-18 /pmc/articles/PMC7489185/ /pubmed/32584721 http://dx.doi.org/10.5606/ehc.2020.74405 Text en Copyright © 2020, Turkish Joint Diseases Foundation http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Article Boz, Mehmet Çakıcı, Hüsamettin Pakdil, Murat Şahin, Abdullah Alper Erdoğan Düzcü, Selma Bala, Mehmet Murat Çelik, Metin Does methylene blue reduce adhesion during the healing process after tendon repair? |
title | Does methylene blue reduce adhesion during the healing process after tendon repair? |
title_full | Does methylene blue reduce adhesion during the healing process after tendon repair? |
title_fullStr | Does methylene blue reduce adhesion during the healing process after tendon repair? |
title_full_unstemmed | Does methylene blue reduce adhesion during the healing process after tendon repair? |
title_short | Does methylene blue reduce adhesion during the healing process after tendon repair? |
title_sort | does methylene blue reduce adhesion during the healing process after tendon repair? |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489185/ https://www.ncbi.nlm.nih.gov/pubmed/32584721 http://dx.doi.org/10.5606/ehc.2020.74405 |
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