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Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019

Mumps cases continue to occur, also in countries with a relatively high vaccination rate. The last major outbreaks of mumps in the Netherlands were in 2009–2012 and thereafter, only small clusters and single cases were reported. Molecular epidemiology can provide insights in the circulation of mumps...

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Autores principales: Bodewes, Rogier, Reijnen, Linda, Kerkhof, Jeroen, Cremer, Jeroen, Schmitz, Dennis, van Binnendijk, Rob, Veldhuijzen, Irene K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489541/
https://www.ncbi.nlm.nih.gov/pubmed/32925979
http://dx.doi.org/10.1371/journal.pone.0233143
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author Bodewes, Rogier
Reijnen, Linda
Kerkhof, Jeroen
Cremer, Jeroen
Schmitz, Dennis
van Binnendijk, Rob
Veldhuijzen, Irene K.
author_facet Bodewes, Rogier
Reijnen, Linda
Kerkhof, Jeroen
Cremer, Jeroen
Schmitz, Dennis
van Binnendijk, Rob
Veldhuijzen, Irene K.
author_sort Bodewes, Rogier
collection PubMed
description Mumps cases continue to occur, also in countries with a relatively high vaccination rate. The last major outbreaks of mumps in the Netherlands were in 2009–2012 and thereafter, only small clusters and single cases were reported. Molecular epidemiology can provide insights in the circulation of mumps viruses. The aims of the present study were to analyze the molecular epidemiology of mumps viruses in the Netherlands in 2017–2019 and to compare the phylogenetic trees built from sequence data of near complete mumps virus genomes or from the SH gene and non-coding regions (SH+NCRs). To this end, Sanger sequence data from SH+NCRs were analyzed from 82 mumps genotype G viruses. In addition, near complete genomes were obtained from 10 mumps virus isolates using next-generation sequencing. Analysis of SH+NCRs sequences of mumps genotype G viruses revealed the presence of two major genetic lineages in the Netherlands, which was confirmed by analysis of near complete genomes. Comparison of phylogenetic trees built with SH+NCRs or near complete genomes indicated that the topology was similar, while somewhat longer branches were present in the phylogenetic tree with near complete genomes. These results confirm that analysis of SH + NCRs sequence data is a useful approach for molecular surveillance. Furthermore, data from recent mumps genotype G viruses might indicate (intermittent) circulation of mumps genotype G viruses in the Netherlands in 2017–2019.
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spelling pubmed-74895412020-09-22 Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019 Bodewes, Rogier Reijnen, Linda Kerkhof, Jeroen Cremer, Jeroen Schmitz, Dennis van Binnendijk, Rob Veldhuijzen, Irene K. PLoS One Research Article Mumps cases continue to occur, also in countries with a relatively high vaccination rate. The last major outbreaks of mumps in the Netherlands were in 2009–2012 and thereafter, only small clusters and single cases were reported. Molecular epidemiology can provide insights in the circulation of mumps viruses. The aims of the present study were to analyze the molecular epidemiology of mumps viruses in the Netherlands in 2017–2019 and to compare the phylogenetic trees built from sequence data of near complete mumps virus genomes or from the SH gene and non-coding regions (SH+NCRs). To this end, Sanger sequence data from SH+NCRs were analyzed from 82 mumps genotype G viruses. In addition, near complete genomes were obtained from 10 mumps virus isolates using next-generation sequencing. Analysis of SH+NCRs sequences of mumps genotype G viruses revealed the presence of two major genetic lineages in the Netherlands, which was confirmed by analysis of near complete genomes. Comparison of phylogenetic trees built with SH+NCRs or near complete genomes indicated that the topology was similar, while somewhat longer branches were present in the phylogenetic tree with near complete genomes. These results confirm that analysis of SH + NCRs sequence data is a useful approach for molecular surveillance. Furthermore, data from recent mumps genotype G viruses might indicate (intermittent) circulation of mumps genotype G viruses in the Netherlands in 2017–2019. Public Library of Science 2020-09-14 /pmc/articles/PMC7489541/ /pubmed/32925979 http://dx.doi.org/10.1371/journal.pone.0233143 Text en © 2020 Bodewes et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bodewes, Rogier
Reijnen, Linda
Kerkhof, Jeroen
Cremer, Jeroen
Schmitz, Dennis
van Binnendijk, Rob
Veldhuijzen, Irene K.
Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019
title Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019
title_full Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019
title_fullStr Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019
title_full_unstemmed Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019
title_short Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019
title_sort molecular epidemiology of mumps viruses in the netherlands, 2017-2019
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489541/
https://www.ncbi.nlm.nih.gov/pubmed/32925979
http://dx.doi.org/10.1371/journal.pone.0233143
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