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STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6

Emerging studies indicate that long noncoding RNAs (lncRNAs) play crucial roles in ovarian cancer (OC). By analyzing high-throughput data, we found that SNHG17 was highly expressed in multiple OC cohorts. However, its functions in OC were not explored. In this study, lncRNA expression in OC was anal...

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Autores principales: Pan, Xuefeng, Guo, Zhiheng, Chen, Yanyan, Zheng, Shu, Peng, Min, Yang, Yi, Wang, Zhenpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490451/
https://www.ncbi.nlm.nih.gov/pubmed/32911343
http://dx.doi.org/10.1016/j.omtn.2020.08.006
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author Pan, Xuefeng
Guo, Zhiheng
Chen, Yanyan
Zheng, Shu
Peng, Min
Yang, Yi
Wang, Zhenpeng
author_facet Pan, Xuefeng
Guo, Zhiheng
Chen, Yanyan
Zheng, Shu
Peng, Min
Yang, Yi
Wang, Zhenpeng
author_sort Pan, Xuefeng
collection PubMed
description Emerging studies indicate that long noncoding RNAs (lncRNAs) play crucial roles in ovarian cancer (OC). By analyzing high-throughput data, we found that SNHG17 was highly expressed in multiple OC cohorts. However, its functions in OC were not explored. In this study, lncRNA expression in OC was analyzed by a series of microarray data. The functions of SNHG17 were investigated by various in vitro and in vivo assays. Fluorescence in situ hybridization (FISH), RNA pull-down, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), and luciferase reporter assays were used to reveal the potential mechanisms involved in the effects of SNHG17. We found that SNHG17 was overexpressed in OC and that the oncogenic transcription factor STAT3 was involved in promoting its expression. In addition, high SNHG17 expression was associated with a poor prognosis in OC. Functional analysis revealed that SNHG17 could promote OC cell growth. Mechanistically, SNHG17 was found to be located predominantly in the cytoplasm. It could regulate expression of CDK6, an important cell-cycle regulator, by acting as a molecular sponge for miR-214-3p. In summary, our study suggested that SNHG17 acted as an oncogene in OC, which might serve as a novel target for OC diagnosis and therapy.
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spelling pubmed-74904512020-09-28 STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6 Pan, Xuefeng Guo, Zhiheng Chen, Yanyan Zheng, Shu Peng, Min Yang, Yi Wang, Zhenpeng Mol Ther Nucleic Acids Original Article Emerging studies indicate that long noncoding RNAs (lncRNAs) play crucial roles in ovarian cancer (OC). By analyzing high-throughput data, we found that SNHG17 was highly expressed in multiple OC cohorts. However, its functions in OC were not explored. In this study, lncRNA expression in OC was analyzed by a series of microarray data. The functions of SNHG17 were investigated by various in vitro and in vivo assays. Fluorescence in situ hybridization (FISH), RNA pull-down, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), and luciferase reporter assays were used to reveal the potential mechanisms involved in the effects of SNHG17. We found that SNHG17 was overexpressed in OC and that the oncogenic transcription factor STAT3 was involved in promoting its expression. In addition, high SNHG17 expression was associated with a poor prognosis in OC. Functional analysis revealed that SNHG17 could promote OC cell growth. Mechanistically, SNHG17 was found to be located predominantly in the cytoplasm. It could regulate expression of CDK6, an important cell-cycle regulator, by acting as a molecular sponge for miR-214-3p. In summary, our study suggested that SNHG17 acted as an oncogene in OC, which might serve as a novel target for OC diagnosis and therapy. American Society of Gene & Cell Therapy 2020-08-08 /pmc/articles/PMC7490451/ /pubmed/32911343 http://dx.doi.org/10.1016/j.omtn.2020.08.006 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Pan, Xuefeng
Guo, Zhiheng
Chen, Yanyan
Zheng, Shu
Peng, Min
Yang, Yi
Wang, Zhenpeng
STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6
title STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6
title_full STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6
title_fullStr STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6
title_full_unstemmed STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6
title_short STAT3-Induced lncRNA SNHG17 Exerts Oncogenic Effects on Ovarian Cancer through Regulating CDK6
title_sort stat3-induced lncrna snhg17 exerts oncogenic effects on ovarian cancer through regulating cdk6
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490451/
https://www.ncbi.nlm.nih.gov/pubmed/32911343
http://dx.doi.org/10.1016/j.omtn.2020.08.006
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