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Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study

The present work evaluated the effect of flaxseed oil (FO) against toxicity induced by cadmium chloride (CdCl(2)) in the mouse liver and kidney. Male Swiss albino mice were treated with CdCl(2) (4.5 mg/kg, intraperitoneally) with or without FO at three concentrations (4, 8, 12 mL/kg, orally) for two...

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Autores principales: Diab, Kawthar A., Ibrahim, Noha E., Fahmy, Maha A., Hassan, Emad M., Omara, Enayat A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490469/
https://www.ncbi.nlm.nih.gov/pubmed/32963966
http://dx.doi.org/10.1016/j.toxrep.2020.08.023
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author Diab, Kawthar A.
Ibrahim, Noha E.
Fahmy, Maha A.
Hassan, Emad M.
Omara, Enayat A.
author_facet Diab, Kawthar A.
Ibrahim, Noha E.
Fahmy, Maha A.
Hassan, Emad M.
Omara, Enayat A.
author_sort Diab, Kawthar A.
collection PubMed
description The present work evaluated the effect of flaxseed oil (FO) against toxicity induced by cadmium chloride (CdCl(2)) in the mouse liver and kidney. Male Swiss albino mice were treated with CdCl(2) (4.5 mg/kg, intraperitoneally) with or without FO at three concentrations (4, 8, 12 mL/kg, orally) for two consecutive weeks. To analyze the effects of FO, we used the following techniques: (1) histopathological examination; (2) comet assay; (3) RT-PCR gene expression analysis of tumor necrosis factor (TNF-α) and tumor suppressor protein (p53); and (4) immunohistochemical analysis of caspase-9 protein expression. The gas chromatography-mass spectrometry results showed that FO had a high content of unsaturated fatty acids including, oleic acid, linolenic acid, and linoleic acid. Oral supplementation with FO (12 mL/kg) resulted in a normal histological appearance without alteration in the DNA integrity and gene expression of TNF-α, p53, and caspase-9 in liver and kidney tissues. As expected, CdCl(2) remarkably induced loss of histological integrity, increased DNA comet formation, increased TNF-α and p53 mRNA expression levels and increased the immunoreactivity of caspase-9 expression. When FO was given before administration of CdCl(2), these histopathological defects were reversed; necrosis, degeneration, inflammatory cell infiltration, hemorrhage, Kupffer cells, and pyknotic cells were all reduced. These histological improvements induced by FO were accompanied by reduced DNA breakage, downregulated mRNA expression of TNF-α and p53, and downregulated immunohistochemical expression of caspase-9 protein. In conclusion, FO and its constituents may act as signaling molecules and modify the expression of genes involved in proinflammatory cytokine production (TNF-α), cell cycle arrest (p53), and apoptosis (caspase-9), thereby improving biological activities and health.
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spelling pubmed-74904692020-09-21 Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study Diab, Kawthar A. Ibrahim, Noha E. Fahmy, Maha A. Hassan, Emad M. Omara, Enayat A. Toxicol Rep Regular Article The present work evaluated the effect of flaxseed oil (FO) against toxicity induced by cadmium chloride (CdCl(2)) in the mouse liver and kidney. Male Swiss albino mice were treated with CdCl(2) (4.5 mg/kg, intraperitoneally) with or without FO at three concentrations (4, 8, 12 mL/kg, orally) for two consecutive weeks. To analyze the effects of FO, we used the following techniques: (1) histopathological examination; (2) comet assay; (3) RT-PCR gene expression analysis of tumor necrosis factor (TNF-α) and tumor suppressor protein (p53); and (4) immunohistochemical analysis of caspase-9 protein expression. The gas chromatography-mass spectrometry results showed that FO had a high content of unsaturated fatty acids including, oleic acid, linolenic acid, and linoleic acid. Oral supplementation with FO (12 mL/kg) resulted in a normal histological appearance without alteration in the DNA integrity and gene expression of TNF-α, p53, and caspase-9 in liver and kidney tissues. As expected, CdCl(2) remarkably induced loss of histological integrity, increased DNA comet formation, increased TNF-α and p53 mRNA expression levels and increased the immunoreactivity of caspase-9 expression. When FO was given before administration of CdCl(2), these histopathological defects were reversed; necrosis, degeneration, inflammatory cell infiltration, hemorrhage, Kupffer cells, and pyknotic cells were all reduced. These histological improvements induced by FO were accompanied by reduced DNA breakage, downregulated mRNA expression of TNF-α and p53, and downregulated immunohistochemical expression of caspase-9 protein. In conclusion, FO and its constituents may act as signaling molecules and modify the expression of genes involved in proinflammatory cytokine production (TNF-α), cell cycle arrest (p53), and apoptosis (caspase-9), thereby improving biological activities and health. Elsevier 2020-08-31 /pmc/articles/PMC7490469/ /pubmed/32963966 http://dx.doi.org/10.1016/j.toxrep.2020.08.023 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Article
Diab, Kawthar A.
Ibrahim, Noha E.
Fahmy, Maha A.
Hassan, Emad M.
Omara, Enayat A.
Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study
title Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study
title_full Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study
title_fullStr Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study
title_full_unstemmed Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study
title_short Inhibitory activity of flaxseed oil against CdCl(2) induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study
title_sort inhibitory activity of flaxseed oil against cdcl(2) induced liver and kidney damage: histopathology, genotoxicity, and gene expression study
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490469/
https://www.ncbi.nlm.nih.gov/pubmed/32963966
http://dx.doi.org/10.1016/j.toxrep.2020.08.023
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