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Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion
In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490527/ https://www.ncbi.nlm.nih.gov/pubmed/32963976 http://dx.doi.org/10.1016/j.btre.2020.e00526 |
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author | Ghio, Silvina Bradanini, María B. Garrido, Mercedes M. Ontañon, Ornella M. Piccinni, Florencia E. Marrero Diaz de Villegas, Ruben Talia, Paola M. Campos, Eleonora |
author_facet | Ghio, Silvina Bradanini, María B. Garrido, Mercedes M. Ontañon, Ornella M. Piccinni, Florencia E. Marrero Diaz de Villegas, Ruben Talia, Paola M. Campos, Eleonora |
author_sort | Ghio, Silvina |
collection | PubMed |
description | In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol. |
format | Online Article Text |
id | pubmed-7490527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-74905272020-09-21 Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion Ghio, Silvina Bradanini, María B. Garrido, Mercedes M. Ontañon, Ornella M. Piccinni, Florencia E. Marrero Diaz de Villegas, Ruben Talia, Paola M. Campos, Eleonora Biotechnol Rep (Amst) Research Article In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol. Elsevier 2020-09-05 /pmc/articles/PMC7490527/ /pubmed/32963976 http://dx.doi.org/10.1016/j.btre.2020.e00526 Text en © 2020 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Ghio, Silvina Bradanini, María B. Garrido, Mercedes M. Ontañon, Ornella M. Piccinni, Florencia E. Marrero Diaz de Villegas, Ruben Talia, Paola M. Campos, Eleonora Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion |
title | Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion |
title_full | Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion |
title_fullStr | Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion |
title_full_unstemmed | Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion |
title_short | Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion |
title_sort | synergic activity of cel8pa β-1,4 endoglucanase and bg1pa β-glucosidase from paenibacillus xylanivorans a59 in beta-glucan conversion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490527/ https://www.ncbi.nlm.nih.gov/pubmed/32963976 http://dx.doi.org/10.1016/j.btre.2020.e00526 |
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