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Identification of salinity responsive genes in lavender through cDNA-AFLP

Currently, a global demand exists forlavender as a significant medicinal plant and source of essential oils. Freshwater and arable lands are two major factors that inhibit extensive farming of medicinal plants in Iran. Saline water from seas and salty soil may be new resources for agricultural use,...

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Detalles Bibliográficos
Autores principales: Banikamali, Mania, Soltanloo, Hassan, Ramezanpour, S. Sanaz, Yamchi, Ahad, Sorahinobar, Mona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490537/
https://www.ncbi.nlm.nih.gov/pubmed/32963973
http://dx.doi.org/10.1016/j.btre.2020.e00520
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author Banikamali, Mania
Soltanloo, Hassan
Ramezanpour, S. Sanaz
Yamchi, Ahad
Sorahinobar, Mona
author_facet Banikamali, Mania
Soltanloo, Hassan
Ramezanpour, S. Sanaz
Yamchi, Ahad
Sorahinobar, Mona
author_sort Banikamali, Mania
collection PubMed
description Currently, a global demand exists forlavender as a significant medicinal plant and source of essential oils. Freshwater and arable lands are two major factors that inhibit extensive farming of medicinal plants in Iran. Saline water from seas and salty soil may be new resources for agricultural use, especially for medicinal plants. We sought to extend our knowledge of the Lavandula angustifolia genome and molecular basis of its salinity tolerance by using cDNA amplified fragment length polymorphism (cDNA-AFLP) to investigate the changes in plant transcriptomes in response to NaCl. All identified transcript derived fragments (TDF) were assigned as novel L. angustifolia genes related to signal transduction, regulation of gene expression, alternative splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR analysis of the TDFs in response to different concentrations of NaCl revealed various levels of mRNA of the identified genes in this plant. Our findings provided primary insights into the molecular response of L. angustifolia to salinity.
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spelling pubmed-74905372020-09-21 Identification of salinity responsive genes in lavender through cDNA-AFLP Banikamali, Mania Soltanloo, Hassan Ramezanpour, S. Sanaz Yamchi, Ahad Sorahinobar, Mona Biotechnol Rep (Amst) Research Article Currently, a global demand exists forlavender as a significant medicinal plant and source of essential oils. Freshwater and arable lands are two major factors that inhibit extensive farming of medicinal plants in Iran. Saline water from seas and salty soil may be new resources for agricultural use, especially for medicinal plants. We sought to extend our knowledge of the Lavandula angustifolia genome and molecular basis of its salinity tolerance by using cDNA amplified fragment length polymorphism (cDNA-AFLP) to investigate the changes in plant transcriptomes in response to NaCl. All identified transcript derived fragments (TDF) were assigned as novel L. angustifolia genes related to signal transduction, regulation of gene expression, alternative splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR analysis of the TDFs in response to different concentrations of NaCl revealed various levels of mRNA of the identified genes in this plant. Our findings provided primary insights into the molecular response of L. angustifolia to salinity. Elsevier 2020-08-11 /pmc/articles/PMC7490537/ /pubmed/32963973 http://dx.doi.org/10.1016/j.btre.2020.e00520 Text en © 2020 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Banikamali, Mania
Soltanloo, Hassan
Ramezanpour, S. Sanaz
Yamchi, Ahad
Sorahinobar, Mona
Identification of salinity responsive genes in lavender through cDNA-AFLP
title Identification of salinity responsive genes in lavender through cDNA-AFLP
title_full Identification of salinity responsive genes in lavender through cDNA-AFLP
title_fullStr Identification of salinity responsive genes in lavender through cDNA-AFLP
title_full_unstemmed Identification of salinity responsive genes in lavender through cDNA-AFLP
title_short Identification of salinity responsive genes in lavender through cDNA-AFLP
title_sort identification of salinity responsive genes in lavender through cdna-aflp
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490537/
https://www.ncbi.nlm.nih.gov/pubmed/32963973
http://dx.doi.org/10.1016/j.btre.2020.e00520
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