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Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis

The aim of this study was to investigate the precise clinical use of Sinitang decoction (SNT) in ulcerative colitis (UC). Network pharmacology-based analysis of the drug components–targets–diseases–pathways was used to predict the possible clinical applications of SNT. Next, 2,4,6-trinitrobenzenesul...

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Autores principales: Ji, Enhui, Wang, Tingting, Xu, Jing, Fan, Jianwei, Zhang, Yi, Guan, Yongxia, Yang, Hongjun, Wei, Junying, Zhang, Guimin, Huang, Luqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490561/
https://www.ncbi.nlm.nih.gov/pubmed/32982747
http://dx.doi.org/10.3389/fphar.2020.01337
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author Ji, Enhui
Wang, Tingting
Xu, Jing
Fan, Jianwei
Zhang, Yi
Guan, Yongxia
Yang, Hongjun
Wei, Junying
Zhang, Guimin
Huang, Luqi
author_facet Ji, Enhui
Wang, Tingting
Xu, Jing
Fan, Jianwei
Zhang, Yi
Guan, Yongxia
Yang, Hongjun
Wei, Junying
Zhang, Guimin
Huang, Luqi
author_sort Ji, Enhui
collection PubMed
description The aim of this study was to investigate the precise clinical use of Sinitang decoction (SNT) in ulcerative colitis (UC). Network pharmacology-based analysis of the drug components–targets–diseases–pathways was used to predict the possible clinical applications of SNT. Next, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was used to establish a rat model of UC, and the efficacy of SNT against UC was tested, followed by a proteomic analysis of the specific signatures regulated by SNT against UC. SNT was predicted to be effective in inflammatory bowel disease, UC, and several other diseases. In the rats with UC, SNT decreased the disease activity index and colon mucosal damage index compared to the untreated UC model rats. Additionally, SNT reversed the upregulated levels of serum tumor necrosis factor (TNF)-α, prostaglandin E(2) (PGE(2)), interleukin (IL)-6, and nitric oxide (NO) in UC model rats. The proteomic analysis identified 78 proteins that were differentially regulated by SNT in the rats with UC, which were associated with the Gene Ontology terms sulfur compound binding, calcium ion binding, and Toll-like receptor (TLR)-4 binding. Among these differentially regulated proteins, C-reactive protein (CRP) and collagen alpha-1(XII) chain (COL12A1) were found to be signature proteins associated with the efficacy of SNT against UC. This study represents the first precise investigation of the efficacy and mechanisms of SNT against UC, and shows that SNT is a promising candidate for personalized management of UC.
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spelling pubmed-74905612020-09-25 Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis Ji, Enhui Wang, Tingting Xu, Jing Fan, Jianwei Zhang, Yi Guan, Yongxia Yang, Hongjun Wei, Junying Zhang, Guimin Huang, Luqi Front Pharmacol Pharmacology The aim of this study was to investigate the precise clinical use of Sinitang decoction (SNT) in ulcerative colitis (UC). Network pharmacology-based analysis of the drug components–targets–diseases–pathways was used to predict the possible clinical applications of SNT. Next, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was used to establish a rat model of UC, and the efficacy of SNT against UC was tested, followed by a proteomic analysis of the specific signatures regulated by SNT against UC. SNT was predicted to be effective in inflammatory bowel disease, UC, and several other diseases. In the rats with UC, SNT decreased the disease activity index and colon mucosal damage index compared to the untreated UC model rats. Additionally, SNT reversed the upregulated levels of serum tumor necrosis factor (TNF)-α, prostaglandin E(2) (PGE(2)), interleukin (IL)-6, and nitric oxide (NO) in UC model rats. The proteomic analysis identified 78 proteins that were differentially regulated by SNT in the rats with UC, which were associated with the Gene Ontology terms sulfur compound binding, calcium ion binding, and Toll-like receptor (TLR)-4 binding. Among these differentially regulated proteins, C-reactive protein (CRP) and collagen alpha-1(XII) chain (COL12A1) were found to be signature proteins associated with the efficacy of SNT against UC. This study represents the first precise investigation of the efficacy and mechanisms of SNT against UC, and shows that SNT is a promising candidate for personalized management of UC. Frontiers Media S.A. 2020-08-31 /pmc/articles/PMC7490561/ /pubmed/32982747 http://dx.doi.org/10.3389/fphar.2020.01337 Text en Copyright © 2020 Ji, Wang, Xu, Fan, Zhang, Guan, Yang, Wei, Zhang and Huang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Ji, Enhui
Wang, Tingting
Xu, Jing
Fan, Jianwei
Zhang, Yi
Guan, Yongxia
Yang, Hongjun
Wei, Junying
Zhang, Guimin
Huang, Luqi
Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis
title Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis
title_full Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis
title_fullStr Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis
title_full_unstemmed Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis
title_short Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis
title_sort systematic investigation of the efficacy of sinitang decoction against ulcerative colitis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490561/
https://www.ncbi.nlm.nih.gov/pubmed/32982747
http://dx.doi.org/10.3389/fphar.2020.01337
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