qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parall...

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Autores principales: Giadone, Richard M., Mithal, Aditya, Miller, Gregory J., Matte, Taylor M., Yeung, Anthony K., Dowrey, Todd W., Werder, Rhiannon B., Miller, Nancy S., Andry, Christopher D., Vanuytsel, Kim, Murphy, George J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490627/
https://www.ncbi.nlm.nih.gov/pubmed/32954369
http://dx.doi.org/10.1016/j.xpro.2020.100102
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author Giadone, Richard M.
Mithal, Aditya
Miller, Gregory J.
Matte, Taylor M.
Yeung, Anthony K.
Dowrey, Todd W.
Werder, Rhiannon B.
Miller, Nancy S.
Andry, Christopher D.
Vanuytsel, Kim
Murphy, George J.
author_facet Giadone, Richard M.
Mithal, Aditya
Miller, Gregory J.
Matte, Taylor M.
Yeung, Anthony K.
Dowrey, Todd W.
Werder, Rhiannon B.
Miller, Nancy S.
Andry, Christopher D.
Vanuytsel, Kim
Murphy, George J.
author_sort Giadone, Richard M.
collection PubMed
description The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time. For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020).
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spelling pubmed-74906272020-09-15 qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples Giadone, Richard M. Mithal, Aditya Miller, Gregory J. Matte, Taylor M. Yeung, Anthony K. Dowrey, Todd W. Werder, Rhiannon B. Miller, Nancy S. Andry, Christopher D. Vanuytsel, Kim Murphy, George J. STAR Protoc Protocol The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time. For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020). Elsevier 2020-09-15 /pmc/articles/PMC7490627/ /pubmed/32954369 http://dx.doi.org/10.1016/j.xpro.2020.100102 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Giadone, Richard M.
Mithal, Aditya
Miller, Gregory J.
Matte, Taylor M.
Yeung, Anthony K.
Dowrey, Todd W.
Werder, Rhiannon B.
Miller, Nancy S.
Andry, Christopher D.
Vanuytsel, Kim
Murphy, George J.
qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
title qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
title_full qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
title_fullStr qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
title_full_unstemmed qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
title_short qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
title_sort qrt-pcr platforms for diagnosing and reporting sars-cov-2 infection in human samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490627/
https://www.ncbi.nlm.nih.gov/pubmed/32954369
http://dx.doi.org/10.1016/j.xpro.2020.100102
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