Cargando…
Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles
The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491131/ https://www.ncbi.nlm.nih.gov/pubmed/32933578 http://dx.doi.org/10.1186/s13048-020-00695-8 |
_version_ | 1783582158774861824 |
---|---|
author | Liu, Gang Li, Shubin Ren, Jinyu Wang, Chunyu Zhang, Yaxuan Su, Xiulan Dai, Yanfeng |
author_facet | Liu, Gang Li, Shubin Ren, Jinyu Wang, Chunyu Zhang, Yaxuan Su, Xiulan Dai, Yanfeng |
author_sort | Liu, Gang |
collection | PubMed |
description | The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented with 20, 40 and 60 μg/mL BAPT, respectively. After establishing the in vitro follicle culture, the gene expression levels and hormone levels were quantified. After in vitro maturation, the developmental rates, reactive oxygen species (ROS) production levels and mitochondrial distributions of MII oocytes were investigated, followed by the analyses of embryonic developmental rates after in vitro fertilization. The results showed that BAPT promoted the growth of mouse preantral follicles. Notably, after 14 d of in vitro culture, the levels of 17 β-estradiol and progesterone were up-regulated with BAPT treatments. Moreover, the expression levels of Oct4, Bmp15, GDF9, FOXO3, Zp3, FOXL2, Inhibin alpha, SOD2, Catalase, GPx and Bcl-2 in the developing follicles were significantly up-regulated after BAPT treatments (P < 0.05), while BAPT significantly inhibited the expression levels of BAX (P < 0.05). Following BAPT treatments, the ROS production levels of MII oocytes were decreased while the mitochondrial distributions were significantly enhanced. Furthermore, increased maturation rates, fertilization and embryonic developmental rates were found in these BAPT-treated groups (P < 0.05). These results demonstrated that BAPT significantly improved the development of preantral follicles in vitro by reducing ROS-dependent cellular damages and by enhancing mitochondrial distributions, thereby promoting the further applications of animal-derived BAPT in biomedical research. |
format | Online Article Text |
id | pubmed-7491131 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74911312020-09-16 Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles Liu, Gang Li, Shubin Ren, Jinyu Wang, Chunyu Zhang, Yaxuan Su, Xiulan Dai, Yanfeng J Ovarian Res Research The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented with 20, 40 and 60 μg/mL BAPT, respectively. After establishing the in vitro follicle culture, the gene expression levels and hormone levels were quantified. After in vitro maturation, the developmental rates, reactive oxygen species (ROS) production levels and mitochondrial distributions of MII oocytes were investigated, followed by the analyses of embryonic developmental rates after in vitro fertilization. The results showed that BAPT promoted the growth of mouse preantral follicles. Notably, after 14 d of in vitro culture, the levels of 17 β-estradiol and progesterone were up-regulated with BAPT treatments. Moreover, the expression levels of Oct4, Bmp15, GDF9, FOXO3, Zp3, FOXL2, Inhibin alpha, SOD2, Catalase, GPx and Bcl-2 in the developing follicles were significantly up-regulated after BAPT treatments (P < 0.05), while BAPT significantly inhibited the expression levels of BAX (P < 0.05). Following BAPT treatments, the ROS production levels of MII oocytes were decreased while the mitochondrial distributions were significantly enhanced. Furthermore, increased maturation rates, fertilization and embryonic developmental rates were found in these BAPT-treated groups (P < 0.05). These results demonstrated that BAPT significantly improved the development of preantral follicles in vitro by reducing ROS-dependent cellular damages and by enhancing mitochondrial distributions, thereby promoting the further applications of animal-derived BAPT in biomedical research. BioMed Central 2020-09-15 /pmc/articles/PMC7491131/ /pubmed/32933578 http://dx.doi.org/10.1186/s13048-020-00695-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Liu, Gang Li, Shubin Ren, Jinyu Wang, Chunyu Zhang, Yaxuan Su, Xiulan Dai, Yanfeng Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
title | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
title_full | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
title_fullStr | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
title_full_unstemmed | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
title_short | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
title_sort | effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491131/ https://www.ncbi.nlm.nih.gov/pubmed/32933578 http://dx.doi.org/10.1186/s13048-020-00695-8 |
work_keys_str_mv | AT liugang effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles AT lishubin effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles AT renjinyu effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles AT wangchunyu effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles AT zhangyaxuan effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles AT suxiulan effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles AT daiyanfeng effectofanimalsourcedbioactivepeptidesontheinvitrodevelopmentofmousepreantralfollicles |