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Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression

Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In...

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Autores principales: Lee, Kyubin, Jang, You-Jee, Lee, Hyerim, Kim, Eunbin, Kim, Yeojin, Yoo, Tong-Kewn, Hyun, Tae Kyung, Park, Jae-Il, Yi, Sun-Ju, Kim, Kyunghwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491199/
https://www.ncbi.nlm.nih.gov/pubmed/32781784
http://dx.doi.org/10.3390/biology9080212
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author Lee, Kyubin
Jang, You-Jee
Lee, Hyerim
Kim, Eunbin
Kim, Yeojin
Yoo, Tong-Kewn
Hyun, Tae Kyung
Park, Jae-Il
Yi, Sun-Ju
Kim, Kyunghwan
author_facet Lee, Kyubin
Jang, You-Jee
Lee, Hyerim
Kim, Eunbin
Kim, Yeojin
Yoo, Tong-Kewn
Hyun, Tae Kyung
Park, Jae-Il
Yi, Sun-Ju
Kim, Kyunghwan
author_sort Lee, Kyubin
collection PubMed
description Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In this study, we present the anti-osteoporotic activity of A. distichum extract by inhibiting osteoclast formation. First, we show that the methanolic extract of the leaves of A. distichum, but not extracts of the branches or fruits, significantly inhibits receptor activator of the NF-κB ligand (RANKL)-induced osteoclast differentiation. Second, our transcriptome analysis revealed that the leaf extract (LE) blocks sets of RANKL-mediated osteoclast-related genes. Third, the LE attenuates the phosphorylation of extracellular signal-related kinase. Finally, treatment with the LE effectively prevents postmenopausal bone loss in ovariectomized mice and glucocorticoid-induced osteoporosis in zebrafish. Our findings show that the extract of A. distichum efficiently suppressed osteoclastogenesis by regulating osteoclast-related genes, thus offering a novel therapeutic strategy for osteoporosis.
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spelling pubmed-74911992020-10-02 Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression Lee, Kyubin Jang, You-Jee Lee, Hyerim Kim, Eunbin Kim, Yeojin Yoo, Tong-Kewn Hyun, Tae Kyung Park, Jae-Il Yi, Sun-Ju Kim, Kyunghwan Biology (Basel) Article Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In this study, we present the anti-osteoporotic activity of A. distichum extract by inhibiting osteoclast formation. First, we show that the methanolic extract of the leaves of A. distichum, but not extracts of the branches or fruits, significantly inhibits receptor activator of the NF-κB ligand (RANKL)-induced osteoclast differentiation. Second, our transcriptome analysis revealed that the leaf extract (LE) blocks sets of RANKL-mediated osteoclast-related genes. Third, the LE attenuates the phosphorylation of extracellular signal-related kinase. Finally, treatment with the LE effectively prevents postmenopausal bone loss in ovariectomized mice and glucocorticoid-induced osteoporosis in zebrafish. Our findings show that the extract of A. distichum efficiently suppressed osteoclastogenesis by regulating osteoclast-related genes, thus offering a novel therapeutic strategy for osteoporosis. MDPI 2020-08-06 /pmc/articles/PMC7491199/ /pubmed/32781784 http://dx.doi.org/10.3390/biology9080212 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Kyubin
Jang, You-Jee
Lee, Hyerim
Kim, Eunbin
Kim, Yeojin
Yoo, Tong-Kewn
Hyun, Tae Kyung
Park, Jae-Il
Yi, Sun-Ju
Kim, Kyunghwan
Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression
title Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression
title_full Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression
title_fullStr Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression
title_full_unstemmed Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression
title_short Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression
title_sort transcriptome analysis reveals that abeliophyllum distichum nakai extract inhibits rankl-mediated osteoclastogenensis mainly through suppressing nfatc1 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491199/
https://www.ncbi.nlm.nih.gov/pubmed/32781784
http://dx.doi.org/10.3390/biology9080212
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