Cargando…
Phylogenetic relationships of closely-related phlebotomine sand flies (Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus
BACKGROUND: The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or diff...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491277/ https://www.ncbi.nlm.nih.gov/pubmed/32935751 http://dx.doi.org/10.1590/0074-02760200220 |
_version_ | 1783582190642135040 |
---|---|
author | Moya, Sofía Lorián Pech-May, Angélica Quintana, María Gabriela Manteca-Acosta, Mariana Salomón, Oscar Daniel |
author_facet | Moya, Sofía Lorián Pech-May, Angélica Quintana, María Gabriela Manteca-Acosta, Mariana Salomón, Oscar Daniel |
author_sort | Moya, Sofía Lorián |
collection | PubMed |
description | BACKGROUND: The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details. OBJECTIVES: We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment. METHODS: The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex. FINDINGS: The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species. MAIN CONCLUSIONS: This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank. |
format | Online Article Text |
id | pubmed-7491277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-74912772020-09-21 Phylogenetic relationships of closely-related phlebotomine sand flies (Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus Moya, Sofía Lorián Pech-May, Angélica Quintana, María Gabriela Manteca-Acosta, Mariana Salomón, Oscar Daniel Mem Inst Oswaldo Cruz Original Article BACKGROUND: The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details. OBJECTIVES: We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment. METHODS: The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex. FINDINGS: The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species. MAIN CONCLUSIONS: This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank. Instituto Oswaldo Cruz, Ministério da Saúde 2020-09-14 /pmc/articles/PMC7491277/ /pubmed/32935751 http://dx.doi.org/10.1590/0074-02760200220 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Original Article Moya, Sofía Lorián Pech-May, Angélica Quintana, María Gabriela Manteca-Acosta, Mariana Salomón, Oscar Daniel Phylogenetic relationships of closely-related phlebotomine sand flies (Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus |
title | Phylogenetic relationships of closely-related phlebotomine sand flies
(Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus |
title_full | Phylogenetic relationships of closely-related phlebotomine sand flies
(Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus |
title_fullStr | Phylogenetic relationships of closely-related phlebotomine sand flies
(Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus |
title_full_unstemmed | Phylogenetic relationships of closely-related phlebotomine sand flies
(Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus |
title_short | Phylogenetic relationships of closely-related phlebotomine sand flies
(Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus |
title_sort | phylogenetic relationships of closely-related phlebotomine sand flies
(diptera: psychodidae) of nyssomyia genus and lutzomyia subgenus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491277/ https://www.ncbi.nlm.nih.gov/pubmed/32935751 http://dx.doi.org/10.1590/0074-02760200220 |
work_keys_str_mv | AT moyasofialorian phylogeneticrelationshipsofcloselyrelatedphlebotominesandfliesdipterapsychodidaeofnyssomyiagenusandlutzomyiasubgenus AT pechmayangelica phylogeneticrelationshipsofcloselyrelatedphlebotominesandfliesdipterapsychodidaeofnyssomyiagenusandlutzomyiasubgenus AT quintanamariagabriela phylogeneticrelationshipsofcloselyrelatedphlebotominesandfliesdipterapsychodidaeofnyssomyiagenusandlutzomyiasubgenus AT mantecaacostamariana phylogeneticrelationshipsofcloselyrelatedphlebotominesandfliesdipterapsychodidaeofnyssomyiagenusandlutzomyiasubgenus AT salomonoscardaniel phylogeneticrelationshipsofcloselyrelatedphlebotominesandfliesdipterapsychodidaeofnyssomyiagenusandlutzomyiasubgenus |