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Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation

OBJECTIVE(S): Lapachone is a natural naphthoquinone-derived compound found in Tabebuia avellanedae. It is well-known for its analgesic, anti-inflammatory, anti-microbial, diuretic, and anti-cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this stud...

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Autores principales: Ahmad, Fayyaz, Bibi, Shaheen, Kang, Mincheol, Anees, Mariam, Ansar, Muhammad, Alam, Muhammad Rizwan, Kim, Sun Yeou, Wahedi, Hussain Mustatab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491501/
https://www.ncbi.nlm.nih.gov/pubmed/32963735
http://dx.doi.org/10.22038/ijbms.2020.43706.10275
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author Ahmad, Fayyaz
Bibi, Shaheen
Kang, Mincheol
Anees, Mariam
Ansar, Muhammad
Alam, Muhammad Rizwan
Kim, Sun Yeou
Wahedi, Hussain Mustatab
author_facet Ahmad, Fayyaz
Bibi, Shaheen
Kang, Mincheol
Anees, Mariam
Ansar, Muhammad
Alam, Muhammad Rizwan
Kim, Sun Yeou
Wahedi, Hussain Mustatab
author_sort Ahmad, Fayyaz
collection PubMed
description OBJECTIVE(S): Lapachone is a natural naphthoquinone-derived compound found in Tabebuia avellanedae. It is well-known for its analgesic, anti-inflammatory, anti-microbial, diuretic, and anti-cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this study was to investigate the wound healing activity of naphthoquinones (α-lapachone and β-lapachone) from Handroanthus impetiginosus. MATERIALS AND METHODS: Expression of Sirt3, migration-related proteins (Rac1, Cdc42, α-Pak) and angiogenesis-related protein of vascular endothelial growth factor (VEGF) was monitored using western blot analysis. Blood vessel formation and tissue development were monitored by angiogenesis assay and hematoxylin & eosin (H & E) staining, respectively on mouse skin tissue samples. Both α-lapachone and β-lapachone increased Sirt3 expression in vivo, but only β-lapachone increased Sirt3 expression in vitro. RESULTS: Both the compounds accelerated wound healing in cultured skin cells as well as mouse skin; however, β-lapachone was more effective at lower concentrations. Both of the compounds increased the expression of migration-related proteins both in vitro and in vivo. Similarly, α-lapachone and β-lapachone increased VEGF expression, tissue development and blood vessel formation in mouse skin. CONCLUSION: These findings indicated that α-lapachone and β-lapachone are novel Sirt3 activators, and Sirt3 has a role in wound healing. Thus, Sirt3 and its regulators come out as a novel target and potential drug candidates, respectively in the important field of cutaneous wound healing.
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spelling pubmed-74915012020-09-21 Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation Ahmad, Fayyaz Bibi, Shaheen Kang, Mincheol Anees, Mariam Ansar, Muhammad Alam, Muhammad Rizwan Kim, Sun Yeou Wahedi, Hussain Mustatab Iran J Basic Med Sci Original Article OBJECTIVE(S): Lapachone is a natural naphthoquinone-derived compound found in Tabebuia avellanedae. It is well-known for its analgesic, anti-inflammatory, anti-microbial, diuretic, and anti-cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this study was to investigate the wound healing activity of naphthoquinones (α-lapachone and β-lapachone) from Handroanthus impetiginosus. MATERIALS AND METHODS: Expression of Sirt3, migration-related proteins (Rac1, Cdc42, α-Pak) and angiogenesis-related protein of vascular endothelial growth factor (VEGF) was monitored using western blot analysis. Blood vessel formation and tissue development were monitored by angiogenesis assay and hematoxylin & eosin (H & E) staining, respectively on mouse skin tissue samples. Both α-lapachone and β-lapachone increased Sirt3 expression in vivo, but only β-lapachone increased Sirt3 expression in vitro. RESULTS: Both the compounds accelerated wound healing in cultured skin cells as well as mouse skin; however, β-lapachone was more effective at lower concentrations. Both of the compounds increased the expression of migration-related proteins both in vitro and in vivo. Similarly, α-lapachone and β-lapachone increased VEGF expression, tissue development and blood vessel formation in mouse skin. CONCLUSION: These findings indicated that α-lapachone and β-lapachone are novel Sirt3 activators, and Sirt3 has a role in wound healing. Thus, Sirt3 and its regulators come out as a novel target and potential drug candidates, respectively in the important field of cutaneous wound healing. Mashhad University of Medical Sciences 2020-09 /pmc/articles/PMC7491501/ /pubmed/32963735 http://dx.doi.org/10.22038/ijbms.2020.43706.10275 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ahmad, Fayyaz
Bibi, Shaheen
Kang, Mincheol
Anees, Mariam
Ansar, Muhammad
Alam, Muhammad Rizwan
Kim, Sun Yeou
Wahedi, Hussain Mustatab
Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
title Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
title_full Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
title_fullStr Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
title_full_unstemmed Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
title_short Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
title_sort naphthoquinones from handroanthus impetiginosus promote skin wound healing through sirt3 regulation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491501/
https://www.ncbi.nlm.nih.gov/pubmed/32963735
http://dx.doi.org/10.22038/ijbms.2020.43706.10275
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