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Canine visceral leishmaniasis in area with recent Leishmania transmission: prevalence, diagnosis, and molecular identification of the infecting species
INTRODUCTION: Canine visceral leishmaniasis (CVL) is an endemic disease in Brazil, and integrated control actions have been adopted by the Brazilian Ministry of Health to control its spread. However, the transmission profile is unknown in areas with recent CVL cases, including Itaúna, located in the...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Medicina Tropical - SBMT
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491567/ https://www.ncbi.nlm.nih.gov/pubmed/32935783 http://dx.doi.org/10.1590/0037-8682-0141-2020 |
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author | Lopes, Josiane Valadão Michalsky, Érika Monteiro Pereira, Nathália Cristina Lima de Paula, Adão Junior Viana Souza, Andreza Geisiane Maia Pinheiro, Letícia Cavalari Lima, Ana Cristina Vianna Mariano da Rocha de Avelar, Daniel Moreira França-Silva, João Carlos Lanzetta, Virgínia Aguiar Sorice de Melo, Jarbas Fortes-Dias, Consuelo Latorre Dias, Edelberto Santos |
author_facet | Lopes, Josiane Valadão Michalsky, Érika Monteiro Pereira, Nathália Cristina Lima de Paula, Adão Junior Viana Souza, Andreza Geisiane Maia Pinheiro, Letícia Cavalari Lima, Ana Cristina Vianna Mariano da Rocha de Avelar, Daniel Moreira França-Silva, João Carlos Lanzetta, Virgínia Aguiar Sorice de Melo, Jarbas Fortes-Dias, Consuelo Latorre Dias, Edelberto Santos |
author_sort | Lopes, Josiane Valadão |
collection | PubMed |
description | INTRODUCTION: Canine visceral leishmaniasis (CVL) is an endemic disease in Brazil, and integrated control actions have been adopted by the Brazilian Ministry of Health to control its spread. However, the transmission profile is unknown in areas with recent CVL cases, including Itaúna, located in the Brazilian state of Minas Gerais, where the present study was carried out. METHODS: A total of 2,302 dogs from 12 neighborhoods were serologically tested for canine VL using the current diagnostic protocol adopted by the Brazilian Ministry of Health. Test positivity rate (TPR) and CVL prevalence were determined for each neighborhood. The presence of Leishmania was assessed in 60 seropositive dogs which had been recommended for euthanasia. Twenty-two of them (37%) were asymptomatic, and 38 (63%) were symptomatic for CVL. Parasitological (myeloculture and smear/imprint) and molecular (PCR) methods were employed for Leishmania detection in bone marrow, spleen, mesenteric lymph nodes, and ear skin. The infecting Leishmania species was identified by DNA sequencing. RESULTS: CVL prevalence (per 1,000 dogs) varied from 0.0-166.67, depending on the neighborhood, with a mean of 68.96 (SD 51.38). Leishmania DNA was detected in at least one tissue from all seropositive dogs, with comparable TPR among tissues. Leishmania parasites were identified in most (54/60) seropositive dogs, and the infecting parasite was identified as Leishmania infantum in all of these. CONCLUSIONS: Prevalence of CVL is a contributor to the spread of visceral leishmaniasis in Itaúna. |
format | Online Article Text |
id | pubmed-7491567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Sociedade Brasileira de Medicina Tropical - SBMT |
record_format | MEDLINE/PubMed |
spelling | pubmed-74915672020-09-16 Canine visceral leishmaniasis in area with recent Leishmania transmission: prevalence, diagnosis, and molecular identification of the infecting species Lopes, Josiane Valadão Michalsky, Érika Monteiro Pereira, Nathália Cristina Lima de Paula, Adão Junior Viana Souza, Andreza Geisiane Maia Pinheiro, Letícia Cavalari Lima, Ana Cristina Vianna Mariano da Rocha de Avelar, Daniel Moreira França-Silva, João Carlos Lanzetta, Virgínia Aguiar Sorice de Melo, Jarbas Fortes-Dias, Consuelo Latorre Dias, Edelberto Santos Rev Soc Bras Med Trop Major Article INTRODUCTION: Canine visceral leishmaniasis (CVL) is an endemic disease in Brazil, and integrated control actions have been adopted by the Brazilian Ministry of Health to control its spread. However, the transmission profile is unknown in areas with recent CVL cases, including Itaúna, located in the Brazilian state of Minas Gerais, where the present study was carried out. METHODS: A total of 2,302 dogs from 12 neighborhoods were serologically tested for canine VL using the current diagnostic protocol adopted by the Brazilian Ministry of Health. Test positivity rate (TPR) and CVL prevalence were determined for each neighborhood. The presence of Leishmania was assessed in 60 seropositive dogs which had been recommended for euthanasia. Twenty-two of them (37%) were asymptomatic, and 38 (63%) were symptomatic for CVL. Parasitological (myeloculture and smear/imprint) and molecular (PCR) methods were employed for Leishmania detection in bone marrow, spleen, mesenteric lymph nodes, and ear skin. The infecting Leishmania species was identified by DNA sequencing. RESULTS: CVL prevalence (per 1,000 dogs) varied from 0.0-166.67, depending on the neighborhood, with a mean of 68.96 (SD 51.38). Leishmania DNA was detected in at least one tissue from all seropositive dogs, with comparable TPR among tissues. Leishmania parasites were identified in most (54/60) seropositive dogs, and the infecting parasite was identified as Leishmania infantum in all of these. CONCLUSIONS: Prevalence of CVL is a contributor to the spread of visceral leishmaniasis in Itaúna. Sociedade Brasileira de Medicina Tropical - SBMT 2020-09-11 /pmc/articles/PMC7491567/ /pubmed/32935783 http://dx.doi.org/10.1590/0037-8682-0141-2020 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Major Article Lopes, Josiane Valadão Michalsky, Érika Monteiro Pereira, Nathália Cristina Lima de Paula, Adão Junior Viana Souza, Andreza Geisiane Maia Pinheiro, Letícia Cavalari Lima, Ana Cristina Vianna Mariano da Rocha de Avelar, Daniel Moreira França-Silva, João Carlos Lanzetta, Virgínia Aguiar Sorice de Melo, Jarbas Fortes-Dias, Consuelo Latorre Dias, Edelberto Santos Canine visceral leishmaniasis in area with recent Leishmania transmission: prevalence, diagnosis, and molecular identification of the infecting species |
title | Canine visceral leishmaniasis in area with recent
Leishmania transmission: prevalence, diagnosis, and
molecular identification of the infecting species |
title_full | Canine visceral leishmaniasis in area with recent
Leishmania transmission: prevalence, diagnosis, and
molecular identification of the infecting species |
title_fullStr | Canine visceral leishmaniasis in area with recent
Leishmania transmission: prevalence, diagnosis, and
molecular identification of the infecting species |
title_full_unstemmed | Canine visceral leishmaniasis in area with recent
Leishmania transmission: prevalence, diagnosis, and
molecular identification of the infecting species |
title_short | Canine visceral leishmaniasis in area with recent
Leishmania transmission: prevalence, diagnosis, and
molecular identification of the infecting species |
title_sort | canine visceral leishmaniasis in area with recent
leishmania transmission: prevalence, diagnosis, and
molecular identification of the infecting species |
topic | Major Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491567/ https://www.ncbi.nlm.nih.gov/pubmed/32935783 http://dx.doi.org/10.1590/0037-8682-0141-2020 |
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