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The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding

Plants have evolved both cell surface-resident receptor-like kinases (RLKs) and intracellular nucleotide-binding leucine-rich repeat (NLR) proteins as immune receptors to detect infections. It remains enigmatic how RLK- and NLR-mediated signaling is connected. Disruption of an immune-activated MEKK1...

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Autores principales: Liu, Jun, Huang, Yanyan, Kong, Liang, Yu, Xiao, Feng, Baomin, Liu, Derui, Zhao, Baoyu, Mendes, Giselle C., Yuan, Peiguo, Ge, Dongdong, Wang, Wen-Ming, Fontes, Elizabeth P. B., Li, Pingwei, Shan, Libo, He, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492416/
https://www.ncbi.nlm.nih.gov/pubmed/32839517
http://dx.doi.org/10.1038/s41477-020-0748-6
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author Liu, Jun
Huang, Yanyan
Kong, Liang
Yu, Xiao
Feng, Baomin
Liu, Derui
Zhao, Baoyu
Mendes, Giselle C.
Yuan, Peiguo
Ge, Dongdong
Wang, Wen-Ming
Fontes, Elizabeth P. B.
Li, Pingwei
Shan, Libo
He, Ping
author_facet Liu, Jun
Huang, Yanyan
Kong, Liang
Yu, Xiao
Feng, Baomin
Liu, Derui
Zhao, Baoyu
Mendes, Giselle C.
Yuan, Peiguo
Ge, Dongdong
Wang, Wen-Ming
Fontes, Elizabeth P. B.
Li, Pingwei
Shan, Libo
He, Ping
author_sort Liu, Jun
collection PubMed
description Plants have evolved both cell surface-resident receptor-like kinases (RLKs) and intracellular nucleotide-binding leucine-rich repeat (NLR) proteins as immune receptors to detect infections. It remains enigmatic how RLK- and NLR-mediated signaling is connected. Disruption of an immune-activated MEKK1-MKK1/2-MPK4 MAPK cascade activates the NLR SUMM2 via the MAPK kinase kinase MEKK2, leading to autoimmunity. To gain insights into the mechanisms underlying SUMM2 activation, we deployed an RNAi-based genetic screen for mekk1 autoimmune suppressors, and identified an uncharacterized malectin-like RLK, named LETUM1 (LET1), as a specific regulator of mekk1-mkk1/2-mpk4 autoimmunity via complexing with both SUMM2 and MEKK2. MEKK2 scaffolds LET1 and SUMM2 for protein stability and association, and counter-regulates the F-box protein CPR1-mediated SUMM2 ubiquitination and degradation; thereby, regulating SUMM2 accumulation and activation. Our study indicates that malectin-like RLK LET1 senses the perturbance of cellular homeostasis caused by the deficiency in immune-activated signaling, and activates the NLR SUMM2-mediated autoimmunity via MEKK2 scaffolding.
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spelling pubmed-74924162021-02-24 The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding Liu, Jun Huang, Yanyan Kong, Liang Yu, Xiao Feng, Baomin Liu, Derui Zhao, Baoyu Mendes, Giselle C. Yuan, Peiguo Ge, Dongdong Wang, Wen-Ming Fontes, Elizabeth P. B. Li, Pingwei Shan, Libo He, Ping Nat Plants Article Plants have evolved both cell surface-resident receptor-like kinases (RLKs) and intracellular nucleotide-binding leucine-rich repeat (NLR) proteins as immune receptors to detect infections. It remains enigmatic how RLK- and NLR-mediated signaling is connected. Disruption of an immune-activated MEKK1-MKK1/2-MPK4 MAPK cascade activates the NLR SUMM2 via the MAPK kinase kinase MEKK2, leading to autoimmunity. To gain insights into the mechanisms underlying SUMM2 activation, we deployed an RNAi-based genetic screen for mekk1 autoimmune suppressors, and identified an uncharacterized malectin-like RLK, named LETUM1 (LET1), as a specific regulator of mekk1-mkk1/2-mpk4 autoimmunity via complexing with both SUMM2 and MEKK2. MEKK2 scaffolds LET1 and SUMM2 for protein stability and association, and counter-regulates the F-box protein CPR1-mediated SUMM2 ubiquitination and degradation; thereby, regulating SUMM2 accumulation and activation. Our study indicates that malectin-like RLK LET1 senses the perturbance of cellular homeostasis caused by the deficiency in immune-activated signaling, and activates the NLR SUMM2-mediated autoimmunity via MEKK2 scaffolding. 2020-08-24 2020-09 /pmc/articles/PMC7492416/ /pubmed/32839517 http://dx.doi.org/10.1038/s41477-020-0748-6 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Liu, Jun
Huang, Yanyan
Kong, Liang
Yu, Xiao
Feng, Baomin
Liu, Derui
Zhao, Baoyu
Mendes, Giselle C.
Yuan, Peiguo
Ge, Dongdong
Wang, Wen-Ming
Fontes, Elizabeth P. B.
Li, Pingwei
Shan, Libo
He, Ping
The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
title The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
title_full The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
title_fullStr The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
title_full_unstemmed The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
title_short The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
title_sort malectin-like receptor-like kinase letum1 modulates nlr protein summ2 activation via mekk2 scaffolding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492416/
https://www.ncbi.nlm.nih.gov/pubmed/32839517
http://dx.doi.org/10.1038/s41477-020-0748-6
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