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The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding
Plants have evolved both cell surface-resident receptor-like kinases (RLKs) and intracellular nucleotide-binding leucine-rich repeat (NLR) proteins as immune receptors to detect infections. It remains enigmatic how RLK- and NLR-mediated signaling is connected. Disruption of an immune-activated MEKK1...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492416/ https://www.ncbi.nlm.nih.gov/pubmed/32839517 http://dx.doi.org/10.1038/s41477-020-0748-6 |
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author | Liu, Jun Huang, Yanyan Kong, Liang Yu, Xiao Feng, Baomin Liu, Derui Zhao, Baoyu Mendes, Giselle C. Yuan, Peiguo Ge, Dongdong Wang, Wen-Ming Fontes, Elizabeth P. B. Li, Pingwei Shan, Libo He, Ping |
author_facet | Liu, Jun Huang, Yanyan Kong, Liang Yu, Xiao Feng, Baomin Liu, Derui Zhao, Baoyu Mendes, Giselle C. Yuan, Peiguo Ge, Dongdong Wang, Wen-Ming Fontes, Elizabeth P. B. Li, Pingwei Shan, Libo He, Ping |
author_sort | Liu, Jun |
collection | PubMed |
description | Plants have evolved both cell surface-resident receptor-like kinases (RLKs) and intracellular nucleotide-binding leucine-rich repeat (NLR) proteins as immune receptors to detect infections. It remains enigmatic how RLK- and NLR-mediated signaling is connected. Disruption of an immune-activated MEKK1-MKK1/2-MPK4 MAPK cascade activates the NLR SUMM2 via the MAPK kinase kinase MEKK2, leading to autoimmunity. To gain insights into the mechanisms underlying SUMM2 activation, we deployed an RNAi-based genetic screen for mekk1 autoimmune suppressors, and identified an uncharacterized malectin-like RLK, named LETUM1 (LET1), as a specific regulator of mekk1-mkk1/2-mpk4 autoimmunity via complexing with both SUMM2 and MEKK2. MEKK2 scaffolds LET1 and SUMM2 for protein stability and association, and counter-regulates the F-box protein CPR1-mediated SUMM2 ubiquitination and degradation; thereby, regulating SUMM2 accumulation and activation. Our study indicates that malectin-like RLK LET1 senses the perturbance of cellular homeostasis caused by the deficiency in immune-activated signaling, and activates the NLR SUMM2-mediated autoimmunity via MEKK2 scaffolding. |
format | Online Article Text |
id | pubmed-7492416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-74924162021-02-24 The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding Liu, Jun Huang, Yanyan Kong, Liang Yu, Xiao Feng, Baomin Liu, Derui Zhao, Baoyu Mendes, Giselle C. Yuan, Peiguo Ge, Dongdong Wang, Wen-Ming Fontes, Elizabeth P. B. Li, Pingwei Shan, Libo He, Ping Nat Plants Article Plants have evolved both cell surface-resident receptor-like kinases (RLKs) and intracellular nucleotide-binding leucine-rich repeat (NLR) proteins as immune receptors to detect infections. It remains enigmatic how RLK- and NLR-mediated signaling is connected. Disruption of an immune-activated MEKK1-MKK1/2-MPK4 MAPK cascade activates the NLR SUMM2 via the MAPK kinase kinase MEKK2, leading to autoimmunity. To gain insights into the mechanisms underlying SUMM2 activation, we deployed an RNAi-based genetic screen for mekk1 autoimmune suppressors, and identified an uncharacterized malectin-like RLK, named LETUM1 (LET1), as a specific regulator of mekk1-mkk1/2-mpk4 autoimmunity via complexing with both SUMM2 and MEKK2. MEKK2 scaffolds LET1 and SUMM2 for protein stability and association, and counter-regulates the F-box protein CPR1-mediated SUMM2 ubiquitination and degradation; thereby, regulating SUMM2 accumulation and activation. Our study indicates that malectin-like RLK LET1 senses the perturbance of cellular homeostasis caused by the deficiency in immune-activated signaling, and activates the NLR SUMM2-mediated autoimmunity via MEKK2 scaffolding. 2020-08-24 2020-09 /pmc/articles/PMC7492416/ /pubmed/32839517 http://dx.doi.org/10.1038/s41477-020-0748-6 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Liu, Jun Huang, Yanyan Kong, Liang Yu, Xiao Feng, Baomin Liu, Derui Zhao, Baoyu Mendes, Giselle C. Yuan, Peiguo Ge, Dongdong Wang, Wen-Ming Fontes, Elizabeth P. B. Li, Pingwei Shan, Libo He, Ping The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding |
title | The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding |
title_full | The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding |
title_fullStr | The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding |
title_full_unstemmed | The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding |
title_short | The malectin-like receptor-like kinase LETUM1 modulates NLR protein SUMM2 activation via MEKK2 scaffolding |
title_sort | malectin-like receptor-like kinase letum1 modulates nlr protein summ2 activation via mekk2 scaffolding |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492416/ https://www.ncbi.nlm.nih.gov/pubmed/32839517 http://dx.doi.org/10.1038/s41477-020-0748-6 |
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