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Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization

PURPOSE: Food allergy is a hypersensitive immune response to specific food proteins. Chitinase 3-like 1 (CHI3L1, also known as YKL-40 in humans or BRP-39 in mice) is associated with various chronic diseases, such as cancer, rheumatoid arthritis, and allergic disease. CHI3L1 is involved in allergen s...

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Autores principales: Kim, Eun Gyul, Kim, Mi Na, Hong, Jung Yeon, Lee, Jae Woo, Kim, Soo Yeon, Kim, Kyung Won, Lee, Chun Geun, Elias, Jack A., Song, Tae Won, Sohn, Myung Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492506/
https://www.ncbi.nlm.nih.gov/pubmed/32935492
http://dx.doi.org/10.4168/aair.2020.12.6.1012
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author Kim, Eun Gyul
Kim, Mi Na
Hong, Jung Yeon
Lee, Jae Woo
Kim, Soo Yeon
Kim, Kyung Won
Lee, Chun Geun
Elias, Jack A.
Song, Tae Won
Sohn, Myung Hyun
author_facet Kim, Eun Gyul
Kim, Mi Na
Hong, Jung Yeon
Lee, Jae Woo
Kim, Soo Yeon
Kim, Kyung Won
Lee, Chun Geun
Elias, Jack A.
Song, Tae Won
Sohn, Myung Hyun
author_sort Kim, Eun Gyul
collection PubMed
description PURPOSE: Food allergy is a hypersensitive immune response to specific food proteins. Chitinase 3-like 1 (CHI3L1, also known as YKL-40 in humans or BRP-39 in mice) is associated with various chronic diseases, such as cancer, rheumatoid arthritis, and allergic disease. CHI3L1 is involved in allergen sensitization and type 2 helper T (Th2) inflammation, but the role of CHI3L1 in food allergy remains unclear. In this study, we sought to investigate the role of CHI3L1 in the development of food allergy. METHODS: We measured serum levels of CHI3L1 in food allergic patients. Food allergy was induced in wild-type (WT) and CHI3L1 null mutant (CHI3L1(−/−)) BALB/c mice with ovalbumin (OVA). We investigated Th2 immune responses, M2 macrophage polarization, and mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase (PI3K) signaling pathways, and also performed transcriptome analysis. RESULTS: Serum levels of CHI3L1 were significantly higher in children with food allergy compared with those in healthy controls. Furthermore, CHI3L1 expression levels were elevated in WT mice after OVA treatment. Food allergy symptoms, immunoglobulin E levels, Th2 cytokine production, and histological injury were attenuated in food allergy-induced CHI3L1(−/−) mice compared with those in food allergy-induced WT mice. CHI3L1 expression was increased in OVA-treated WT intestinal macrophages and caused M2 macrophage polarization. Furthermore, CHI3L1 was involved in the extracellular signal-regulated kinases (ERK) and AKT signaling pathways and was associated with immune response and lipid metabolism as determined through transcriptome analysis. CONCLUSIONS: CHI3L1 plays a pivotal role in Th2 inflammation and M2 macrophage polarization through MAPK/ERK and PI3K/AKT phosphorylation in food allergy.
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spelling pubmed-74925062020-11-01 Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization Kim, Eun Gyul Kim, Mi Na Hong, Jung Yeon Lee, Jae Woo Kim, Soo Yeon Kim, Kyung Won Lee, Chun Geun Elias, Jack A. Song, Tae Won Sohn, Myung Hyun Allergy Asthma Immunol Res Original Article PURPOSE: Food allergy is a hypersensitive immune response to specific food proteins. Chitinase 3-like 1 (CHI3L1, also known as YKL-40 in humans or BRP-39 in mice) is associated with various chronic diseases, such as cancer, rheumatoid arthritis, and allergic disease. CHI3L1 is involved in allergen sensitization and type 2 helper T (Th2) inflammation, but the role of CHI3L1 in food allergy remains unclear. In this study, we sought to investigate the role of CHI3L1 in the development of food allergy. METHODS: We measured serum levels of CHI3L1 in food allergic patients. Food allergy was induced in wild-type (WT) and CHI3L1 null mutant (CHI3L1(−/−)) BALB/c mice with ovalbumin (OVA). We investigated Th2 immune responses, M2 macrophage polarization, and mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase (PI3K) signaling pathways, and also performed transcriptome analysis. RESULTS: Serum levels of CHI3L1 were significantly higher in children with food allergy compared with those in healthy controls. Furthermore, CHI3L1 expression levels were elevated in WT mice after OVA treatment. Food allergy symptoms, immunoglobulin E levels, Th2 cytokine production, and histological injury were attenuated in food allergy-induced CHI3L1(−/−) mice compared with those in food allergy-induced WT mice. CHI3L1 expression was increased in OVA-treated WT intestinal macrophages and caused M2 macrophage polarization. Furthermore, CHI3L1 was involved in the extracellular signal-regulated kinases (ERK) and AKT signaling pathways and was associated with immune response and lipid metabolism as determined through transcriptome analysis. CONCLUSIONS: CHI3L1 plays a pivotal role in Th2 inflammation and M2 macrophage polarization through MAPK/ERK and PI3K/AKT phosphorylation in food allergy. The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2020-07-13 /pmc/articles/PMC7492506/ /pubmed/32935492 http://dx.doi.org/10.4168/aair.2020.12.6.1012 Text en Copyright © 2020 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Eun Gyul
Kim, Mi Na
Hong, Jung Yeon
Lee, Jae Woo
Kim, Soo Yeon
Kim, Kyung Won
Lee, Chun Geun
Elias, Jack A.
Song, Tae Won
Sohn, Myung Hyun
Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization
title Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization
title_full Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization
title_fullStr Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization
title_full_unstemmed Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization
title_short Chitinase 3-Like 1 Contributes to Food Allergy via M2 Macrophage Polarization
title_sort chitinase 3-like 1 contributes to food allergy via m2 macrophage polarization
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492506/
https://www.ncbi.nlm.nih.gov/pubmed/32935492
http://dx.doi.org/10.4168/aair.2020.12.6.1012
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