Cargando…

Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using Error-Prone PCR

Lipases catalyze the hydrolysis of fats and oils, and have been widely used in various industrial fields. However, bacterial lipases have a lower thermostability in industrial processes, which was a limiting factor in their industrial application. In this study, we obtained an improve variant of Pse...

Descripción completa

Detalles Bibliográficos
Autores principales:	Guan, Lijun, Gao, Yang, Li, Jialei, Wang, Kunlun, Zhang, Zhihong, Yan, Song, Ji, Nina, Zhou, Ye, Lu, Shuwen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492553/ https://www.ncbi.nlm.nih.gov/pubmed/32984290 http://dx.doi.org/10.3389/fbioe.2020.01034

Ejemplares similares

Corrigendum: Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using Error-Prone PCR
por: Guan, Lijun, et al.
Publicado: (2020)

Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis
por: Xing, Hongguan, et al.
Publicado: (2023)

Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads
por: Arana-Peña, Sara, et al.
Publicado: (2020)

Biochemical and Structural Characterization of a Novel Bacterial Tannase From Lachnospiraceae bacterium in Ruminant Gastrointestinal Tract
por: Guan, Lijun, et al.
Publicado: (2021)

Enhancement of Activity and Thermostability of Keratinase From Pseudomonas aeruginosa CCTCC AB2013184 by Directed Evolution With Noncanonical Amino Acids
por: Pan, Xianchao, et al.
Publicado: (2021)

An image cryptography method by highly error-prone DNA storage channel
por: Zan, Xiangzhen, et al.
Publicado: (2023)

Characterization of Polymer Degrading Lipases, LIP1 and LIP2 From Pseudomonas chlororaphis PA23
por: Mohanan, Nisha, et al.
Publicado: (2022)

Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study
por: Zhang, Weiqing, et al.
Publicado: (2018)

Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids
por: Hu, Zhiming, et al.
Publicado: (2023)

Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective
por: Ajeje, Samaila Boyi, et al.
Publicado: (2021)

Optimization of the Lipase-Catalyzed Selective Amidation of Phenylglycinol
por: Sun, Meina, et al.
Publicado: (2020)

Biochemical and Structural Properties of a Thermostable Mercuric Ion Reductase from Metallosphaera sedula
por: Artz, Jacob H., et al.
Publicado: (2015)

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13
por: Yadav, Punam, et al.
Publicado: (2018)

Purification and Characterization of Nit(phym), a Robust Thermostable Nitrilase From Paraburkholderia phymatum
por: Bessonnet, Thomas, et al.
Publicado: (2021)

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases
por: Nawaz, Ali, et al.
Publicado: (2023)

Optimization of lipase production on agro-industrial residue medium by Pseudomonas fluorescens (NRLL B-2641) using response surface methodology
por: Tanyol, Mehtap, et al.
Publicado: (2015)

Improving Thermostability of Chimeric Enzymes Generated by Domain Shuffling Between Two Different Original Glucoamylases
por: Chen, Zhongxiu, et al.
Publicado: (2022)

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties
por: Khan, Faez Iqbal, et al.
Publicado: (2017)

Cell Surface Display of Thermomyces lanuginosus Lipase in Pichia pastoris
por: Yang, Jiaming, et al.
Publicado: (2020)

Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
por: Bartha-Vári, Judith-Hajnal, et al.
Publicado: (2020)

Thermostable DNA Ligase-Mediated PCR Production of Circular Plasmid (PPCP) and Its Application in Directed Evolution via In situ Error-Prone PCR
por: Le, Yilin, et al.
Publicado: (2013)

Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon
por: Leis, Benedikt, et al.
Publicado: (2015)

Expression, Characterization and Its Deinking Potential of a Thermostable Xylanase From Planomicrobium glaciei CHR43
por: Liu, Zhaoxing, et al.
Publicado: (2021)

A thermostable and CBM2-linked GH10 xylanase from Thermobifida fusca for paper bleaching
por: Wu, Xiuyun, et al.
Publicado: (2022)

An Insight Into Ameliorating Production, Catalytic Efficiency, Thermostability and Starch Saccharification of Acid-Stable α-Amylases From Acidophiles
por: Parashar, Deepak, et al.
Publicado: (2018)

Enhancing the Thermostability of Papain by Immobilizing on Deep Eutectic Solvents-Treated Chitosan With Optimal Microporous Structure and Catalytic Microenvironment
por: Lin, Kai-Peng, et al.
Publicado: (2020)

N-Ribosyltransferase From Archaeoglobus veneficus: A Novel Halotolerant and Thermostable Biocatalyst for the Synthesis of Purine Ribonucleoside Analogs
por: Acosta, Javier, et al.
Publicado: (2020)

Engineering Cofactor Specificity of a Thermostable Phosphite Dehydrogenase for a Highly Efficient and Robust NADPH Regeneration System
por: Abdel-Hady, Gamal Nasser, et al.
Publicado: (2021)

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis
por: Chen, Meiqi, et al.
Publicado: (2022)

Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis
por: Zhang, Ning, et al.
Publicado: (2023)

Biochemical purification and characterization of a truncated acidic, thermostable chitinase from marine fungus for N-acetylglucosamine production
por: He, Bin, et al.
Publicado: (2022)

Solvent-Free Lipase-Catalyzed Synthesis of Diacylgycerols as Low-Calorie Food Ingredients
por: Vázquez, Luis, et al.
Publicado: (2016)

Molecular Dynamic Simulation of the Porcine Pancreatic Lipase in Non-aqueous Organic Solvents
por: Chen, Zi-Shi, et al.
Publicado: (2020)

A Novel Fungal Lipase With Methanol Tolerance and Preference for Macaw Palm Oil
por: Rade, Letícia L., et al.
Publicado: (2020)

Highly Thermostable Xylanase Production from A Thermophilic Geobacillus sp. Strain WSUCF1 Utilizing Lignocellulosic Biomass
por: Bhalla, Aditya, et al.
Publicado: (2015)

Thermostable Branched-Chain Amino Acid Transaminases From the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus: Biochemical and Structural Characterization
por: Isupov, Michail N., et al.
Publicado: (2019)

Characterization and Secretory Expression of a Thermostable Tannase from Aureobasidium melanogenum T9: Potential Candidate for Food and Agricultural Industries
por: Liu, Lu, et al.
Publicado: (2022)

Study of key amino acid residues of GH66 dextranase for producing high-degree polymerized isomaltooligosaccharides and improving of thermostability
por: Lin, Qianru, et al.
Publicado: (2022)

Improved secretory expression and characterization of thermostable xylanase and β-xylosidase from Pseudothermotoga thermarum and their application in synergistic degradation of lignocellulose
por: Chen, Jinkang, et al.
Publicado: (2023)

Modification of fatty acid selectivity of Candida antarctica lipase A by error-prone PCR
por: Głód, Dagmara
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_bglfjovdhb581ao111dklu8mue