Cargando…

Design and Construction of Portable CRISPR-Cpf1-Mediated Genome Editing in Bacillus subtilis 168 Oriented Toward Multiple Utilities

Bacillus subtilis is an important Gram-positive bacterium for industrial biotechnology, which has been widely used to produce diverse high-value added chemicals and industrially and pharmaceutically relevant proteins. Robust and versatile toolkits for genome editing in B. subtilis are highly demandi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hao, Wenliang, Suo, Feiya, Lin, Qiao, Chen, Qiaoqing, Zhou, Li, Liu, Zhongmei, Cui, Wenjing, Zhou, Zhemin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492563/ https://www.ncbi.nlm.nih.gov/pubmed/32984297 http://dx.doi.org/10.3389/fbioe.2020.524676

Ejemplares similares

Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis
por: Han, Laichuang, et al.
Publicado: (2019)

Construction and application of an efficient dual-base editing platform for Bacillus subtilis evolution employing programmable base conversion
por: Hao, Wenliang, et al.
Publicado: (2022)

Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis
por: Cui, Wenjing, et al.
Publicado: (2018)

Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme Maturation via Programming a Controllable Cobalt-Responsive Gene Circuit
por: Han, Laichuang, et al.
Publicado: (2020)

Development of a base editor for protein evolution via in situ mutation in vivo
por: Hao, Wenliang, et al.
Publicado: (2021)

Development of a Glycerol-Inducible Expression System for High-Yield Heterologous Protein Production in Bacillus subtilis
por: Han, Laichuang, et al.
Publicado: (2022)

Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis
por: Guan, Chengran, et al.
Publicado: (2016)

Realization of Robust and Precise Regulation of Gene Expression by Multiple Sigma Recognizable Artificial Promoters
por: Han, Laichuang, et al.
Publicado: (2020)

High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters
por: Liu, Zhongmei, et al.
Publicado: (2019)

Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum
por: Li, Mingyue, et al.
Publicado: (2020)

Enhanced Thermal Stability and Hydrolytic Ability of Bacillus subtilis Aminopeptidase by Removing the Thermal Sensitive Domain in the Non-Catalytic Region
por: Gao, Xinxing, et al.
Publicado: (2014)

CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
por: Jiang, Yu, et al.
Publicado: (2017)

Regulation of heterologous subtilin production in Bacillus subtilis W168
por: Zhang, Qian, et al.
Publicado: (2022)

The Cpf1 CRISPR-Cas protein expands genome-editing tools
por: Fagerlund, Robert D., et al.
Publicado: (2015)

Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing
por: Li, Bin, et al.
Publicado: (2018)

CRISPR Cpf1 proteins: structure, function and implications for genome editing
por: Safari, Fatemeh, et al.
Publicado: (2019)

CRISPR/Cpf1-mediated DNA-free plant genome editing
por: Kim, Hyeran, et al.
Publicado: (2017)

The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants
por: Alok, Anshu, et al.
Publicado: (2020)

Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophylline-activated synthetic riboswitch
por: Cui, Wenjing, et al.
Publicado: (2016)

FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae
por: Świat, Michal A., et al.
Publicado: (2017)

Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae
por: Feng, Qing, et al.
Publicado: (2023)

Inactivation of aprE Gene in Bacillus subtilis 168 by Homologus Recombination
por: Rabbani, Mohammed, et al.
Publicado: (2014)

Intrinsic Levanase Activity of Bacillus subtilis 168 Levansucrase (SacB)
por: Méndez-Lorenzo, Luz, et al.
Publicado: (2015)

Comparative Assessment of Factors Involved in Acetoin Synthesis by Bacillus subtilis 168
por: Sharma, Pratibha, et al.
Publicado: (2014)

The significance of proline and glutamate on butanol chaotropic stress in Bacillus subtilis 168
por: Mahipant, Gumpanat, et al.
Publicado: (2017)

Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs
por: Ji, Shengyue, et al.
Publicado: (2015)

CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae
por: Verwaal, René, et al.
Publicado: (2017)

Survey and Validation of tRNA Modifications and Their Corresponding Genes in Bacillus subtilis sp Subtilis Strain 168
por: de Crécy-Lagard, Valérie, et al.
Publicado: (2020)

Homology modeling of an antifungal metabolite plipastatin synthase from the Bacillus subtilis 168
por: Batool, Maria, et al.
Publicado: (2011)

Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria
por: Ungerer, Justin, et al.
Publicado: (2016)

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
por: Moreno-Mateos, Miguel A., et al.
Publicado: (2017)

Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency
por: Li, Bin, et al.
Publicado: (2017)

Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases
por: Begemann, Matthew B., et al.
Publicado: (2017)

SEVA-Cpf1, a CRISPR-Cas12a vector for genome editing in cyanobacteria
por: Baldanta, Sara, et al.
Publicado: (2022)

Construction and development of an auto-regulatory gene expression system in Bacillus subtilis
por: Guan, Chengran, et al.
Publicado: (2015)

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
por: Bin Moon, Su, et al.
Publicado: (2018)

Gene activation in human cells using CRISPR/Cpf1-p300 and CRISPR/Cpf1-SunTag systems
por: Zhang, Xin, et al.
Publicado: (2017)

Membrane composition and organization of Bacillus subtilis 168 and its genome‐reduced derivative miniBacillus PG10
por: van Tilburg, Amanda Y., et al.
Publicado: (2021)

Towards the Anaerobic Production of Surfactin Using Bacillus subtilis
por: Hoffmann, Mareen, et al.
Publicado: (2020)

Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
por: Garg, Abhroop, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ogore9dfqc8bj4umfi9gtvlmgf