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Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency

Jingui Shenqi Pills (JGSQP) have been a staple of traditional Chinese medicine for thousands of years, used primarily as a treatment for kidney yang deficiency (KYD). In vitro analyses of JGSQP revealed strong induction of osteogenic differentiation and inhibition of adipogenic differentiation in bo...

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Autores principales: Shang, Qi, Zhao, Wenhua, Shen, Gengyang, Yu, Xiang, Zhang, Zhida, Huang, Xuan, Qin, Weicheng, Chen, Guifeng, Yu, Fuyong, Tang, Kai, Chen, Honglin, Li, Juanmin, Liang, De, Tang, Jingjing, Jiang, Xiaobing, Ren, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492893/
https://www.ncbi.nlm.nih.gov/pubmed/32963560
http://dx.doi.org/10.1155/2020/1517596
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author Shang, Qi
Zhao, Wenhua
Shen, Gengyang
Yu, Xiang
Zhang, Zhida
Huang, Xuan
Qin, Weicheng
Chen, Guifeng
Yu, Fuyong
Tang, Kai
Chen, Honglin
Li, Juanmin
Liang, De
Tang, Jingjing
Jiang, Xiaobing
Ren, Hui
author_facet Shang, Qi
Zhao, Wenhua
Shen, Gengyang
Yu, Xiang
Zhang, Zhida
Huang, Xuan
Qin, Weicheng
Chen, Guifeng
Yu, Fuyong
Tang, Kai
Chen, Honglin
Li, Juanmin
Liang, De
Tang, Jingjing
Jiang, Xiaobing
Ren, Hui
author_sort Shang, Qi
collection PubMed
description Jingui Shenqi Pills (JGSQP) have been a staple of traditional Chinese medicine for thousands of years, used primarily as a treatment for kidney yang deficiency (KYD). In vitro analyses of JGSQP revealed strong induction of osteogenic differentiation and inhibition of adipogenic differentiation in bone-marrow-derived mesenchymal stem/stromal cells. However, the mechanisms by which JGSQP regulate the bone-fat balance in murine ovariectomy-induced osteoporosis with KYD have not been reported. Materials and Methods. Two-month-old female C57BL/6 mice were divided randomly into three groups: those receiving a sham operation (Sham); those undergoing bilateral ovariectomy and selection of KYD syndrome (Model); and those subjected to both bilateral ovariectomy and KYD syndrome selection for 8 weeks, followed by JGSQP treatment for 4 weeks (JGSQP). In the Sham and Model groups, mice were given the same dose of distilled water orally for 4 weeks. Animals from all three groups were euthanised at the 12th week. Vertebral microarchitecture and histomorphology were examined by micro-CT and H&E staining, respectively. In addition, we examined the mRNA expression of Akt, Wnt10b, Osterix (Osx), Fndc5, PPARγ, and Fabp4, as well as the protein of AKT, phosphorylation-AKT (p-AKT), BMP2, COL1A1, and FNDC5. Results. JGSQP treatment improved bone microarchitecture and mitigated histomorphological damage relative to the Model group. The osteoblast number (Ob.N/BS) and area (Ob.S/BS) were increased, whereas adipocyte number (adipocyte/tissue area) and area (adipocyte area/tissue area) were decreased in the JGSQP group. JGSQP treatment reduced the mRNA expression of Akt and adipogenesis-related genes (Fndc5, PPARγ, and Fabp4) while promoting osteogenesis-related genes (Wnt10b and Osx) mRNA expression. Additionally, the expression of p-AKT, BMP2, and COL1A1 proteins was increased and FNDC5 protein expression was decreased after JGSQP treatment. Conclusions. JGSQP treatment reversed murine ovariectomy-induced osteoporosis with KYD by controlling bone-fat balance via AKT pathway.
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spelling pubmed-74928932020-09-21 Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency Shang, Qi Zhao, Wenhua Shen, Gengyang Yu, Xiang Zhang, Zhida Huang, Xuan Qin, Weicheng Chen, Guifeng Yu, Fuyong Tang, Kai Chen, Honglin Li, Juanmin Liang, De Tang, Jingjing Jiang, Xiaobing Ren, Hui Evid Based Complement Alternat Med Research Article Jingui Shenqi Pills (JGSQP) have been a staple of traditional Chinese medicine for thousands of years, used primarily as a treatment for kidney yang deficiency (KYD). In vitro analyses of JGSQP revealed strong induction of osteogenic differentiation and inhibition of adipogenic differentiation in bone-marrow-derived mesenchymal stem/stromal cells. However, the mechanisms by which JGSQP regulate the bone-fat balance in murine ovariectomy-induced osteoporosis with KYD have not been reported. Materials and Methods. Two-month-old female C57BL/6 mice were divided randomly into three groups: those receiving a sham operation (Sham); those undergoing bilateral ovariectomy and selection of KYD syndrome (Model); and those subjected to both bilateral ovariectomy and KYD syndrome selection for 8 weeks, followed by JGSQP treatment for 4 weeks (JGSQP). In the Sham and Model groups, mice were given the same dose of distilled water orally for 4 weeks. Animals from all three groups were euthanised at the 12th week. Vertebral microarchitecture and histomorphology were examined by micro-CT and H&E staining, respectively. In addition, we examined the mRNA expression of Akt, Wnt10b, Osterix (Osx), Fndc5, PPARγ, and Fabp4, as well as the protein of AKT, phosphorylation-AKT (p-AKT), BMP2, COL1A1, and FNDC5. Results. JGSQP treatment improved bone microarchitecture and mitigated histomorphological damage relative to the Model group. The osteoblast number (Ob.N/BS) and area (Ob.S/BS) were increased, whereas adipocyte number (adipocyte/tissue area) and area (adipocyte area/tissue area) were decreased in the JGSQP group. JGSQP treatment reduced the mRNA expression of Akt and adipogenesis-related genes (Fndc5, PPARγ, and Fabp4) while promoting osteogenesis-related genes (Wnt10b and Osx) mRNA expression. Additionally, the expression of p-AKT, BMP2, and COL1A1 proteins was increased and FNDC5 protein expression was decreased after JGSQP treatment. Conclusions. JGSQP treatment reversed murine ovariectomy-induced osteoporosis with KYD by controlling bone-fat balance via AKT pathway. Hindawi 2020-09-04 /pmc/articles/PMC7492893/ /pubmed/32963560 http://dx.doi.org/10.1155/2020/1517596 Text en Copyright © 2020 Qi Shang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shang, Qi
Zhao, Wenhua
Shen, Gengyang
Yu, Xiang
Zhang, Zhida
Huang, Xuan
Qin, Weicheng
Chen, Guifeng
Yu, Fuyong
Tang, Kai
Chen, Honglin
Li, Juanmin
Liang, De
Tang, Jingjing
Jiang, Xiaobing
Ren, Hui
Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency
title Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency
title_full Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency
title_fullStr Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency
title_full_unstemmed Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency
title_short Jingui Shenqi Pills Regulate Bone-Fat Balance in Murine Ovariectomy-Induced Osteoporosis with Kidney Yang Deficiency
title_sort jingui shenqi pills regulate bone-fat balance in murine ovariectomy-induced osteoporosis with kidney yang deficiency
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492893/
https://www.ncbi.nlm.nih.gov/pubmed/32963560
http://dx.doi.org/10.1155/2020/1517596
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