Cargando…

Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria

Paper-based test strip consisting of cerium oxide nanoparticles (nanoceria) as hydrogen peroxide (H(2)O(2))-dependent color-changing nanozymes and cholesterol oxidase (ChOx) has been developed for convenient colorimetric determination of cholesterol without the need for chromogenic substrate. The co...

Descripción completa

Detalles Bibliográficos
Autores principales: Nguyen, Phuong Thy, Kim, Young Im, Kim, Moon Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493407/
https://www.ncbi.nlm.nih.gov/pubmed/32984259
http://dx.doi.org/10.3389/fchem.2020.00798
_version_ 1783582562226012160
author Nguyen, Phuong Thy
Kim, Young Im
Kim, Moon Il
author_facet Nguyen, Phuong Thy
Kim, Young Im
Kim, Moon Il
author_sort Nguyen, Phuong Thy
collection PubMed
description Paper-based test strip consisting of cerium oxide nanoparticles (nanoceria) as hydrogen peroxide (H(2)O(2))-dependent color-changing nanozymes and cholesterol oxidase (ChOx) has been developed for convenient colorimetric determination of cholesterol without the need for chromogenic substrate. The construction of the cholesterol strip begins with physical adsorption of nanoceria on the paper surface, followed by covalent immobilization of ChOx via silanization, chitosan-mediated activation, and glutaraldehyde treatment of the nanoceria-embedded paper matrices. In the presence of cholesterol, ChOx catalyzes its oxidation to produce H(2)O(2), which forms peroxide complex on the nanoceria surface and induces visual color change of the nanoceria-embedded paper from white/light yellow into intense yellow/orange, which was conveniently quantified with an image acquired by a conventional smartphone with the ImageJ software. Using this strategy, target cholesterol was specifically determined down to 40 μM with a dynamic linear concentration range of 0.1–1.5 mM under neutral pH condition, which is suitable to measure the serum cholesterol, with excellent stability during 20 days and reusability by recovering its original color-changing activity for 4 consecutive cycles. Furthermore, the practical utility of this strategy was successfully demonstrated by reliably determining cholesterol in human blood serum samples. This study demonstrates the potential of self color-changing nanozymes for developing colorimetric paper strip sensor, which is particularly useful in instrumentation-free point-of-care environments.
format Online
Article
Text
id pubmed-7493407
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-74934072020-09-25 Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria Nguyen, Phuong Thy Kim, Young Im Kim, Moon Il Front Chem Chemistry Paper-based test strip consisting of cerium oxide nanoparticles (nanoceria) as hydrogen peroxide (H(2)O(2))-dependent color-changing nanozymes and cholesterol oxidase (ChOx) has been developed for convenient colorimetric determination of cholesterol without the need for chromogenic substrate. The construction of the cholesterol strip begins with physical adsorption of nanoceria on the paper surface, followed by covalent immobilization of ChOx via silanization, chitosan-mediated activation, and glutaraldehyde treatment of the nanoceria-embedded paper matrices. In the presence of cholesterol, ChOx catalyzes its oxidation to produce H(2)O(2), which forms peroxide complex on the nanoceria surface and induces visual color change of the nanoceria-embedded paper from white/light yellow into intense yellow/orange, which was conveniently quantified with an image acquired by a conventional smartphone with the ImageJ software. Using this strategy, target cholesterol was specifically determined down to 40 μM with a dynamic linear concentration range of 0.1–1.5 mM under neutral pH condition, which is suitable to measure the serum cholesterol, with excellent stability during 20 days and reusability by recovering its original color-changing activity for 4 consecutive cycles. Furthermore, the practical utility of this strategy was successfully demonstrated by reliably determining cholesterol in human blood serum samples. This study demonstrates the potential of self color-changing nanozymes for developing colorimetric paper strip sensor, which is particularly useful in instrumentation-free point-of-care environments. Frontiers Media S.A. 2020-09-02 /pmc/articles/PMC7493407/ /pubmed/32984259 http://dx.doi.org/10.3389/fchem.2020.00798 Text en Copyright © 2020 Nguyen, Kim and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Nguyen, Phuong Thy
Kim, Young Im
Kim, Moon Il
Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria
title Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria
title_full Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria
title_fullStr Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria
title_full_unstemmed Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria
title_short Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria
title_sort reagent-free colorimetric cholesterol test strip based on self color-changing property of nanoceria
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493407/
https://www.ncbi.nlm.nih.gov/pubmed/32984259
http://dx.doi.org/10.3389/fchem.2020.00798
work_keys_str_mv AT nguyenphuongthy reagentfreecolorimetriccholesterolteststripbasedonselfcolorchangingpropertyofnanoceria
AT kimyoungim reagentfreecolorimetriccholesterolteststripbasedonselfcolorchangingpropertyofnanoceria
AT kimmoonil reagentfreecolorimetriccholesterolteststripbasedonselfcolorchangingpropertyofnanoceria