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Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants
BACKGROUND: Plant viruses maintain intricate interactions with their vector and non-vector insects and can impact the fitness of insects. However, the details of their molecular and cellular mechanisms have not been studied well. We compared the transcriptome-level responses in vector and non-vector...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493910/ https://www.ncbi.nlm.nih.gov/pubmed/32933469 http://dx.doi.org/10.1186/s12864-020-07057-0 |
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author | Li, Dandan Zhang, Chi Tong, Zeqian Su, Dan Zhang, Gaisheng Zhang, Shize Zhao, Huiyan Hu, Zuqing |
author_facet | Li, Dandan Zhang, Chi Tong, Zeqian Su, Dan Zhang, Gaisheng Zhang, Shize Zhao, Huiyan Hu, Zuqing |
author_sort | Li, Dandan |
collection | PubMed |
description | BACKGROUND: Plant viruses maintain intricate interactions with their vector and non-vector insects and can impact the fitness of insects. However, the details of their molecular and cellular mechanisms have not been studied well. We compared the transcriptome-level responses in vector and non-vector aphids (Schizaphis graminum and Rhopalosiphum padi, respectively) after feeding on wheat plants with viral infections (Barley Yellow Dwarf Virus (BYDV) and Wheat dwarf virus (WDV), respectively). We conducted differentially expressed gene (DEG) annotation analyses and observed DEGs related to immune pathway, growth, development, and reproduction. And we conducted cloning and bioinformatic analyses of the key DEG involved in immune. RESULTS: For all differentially expressed gene analyses, the numbers of DEGs related to immune, growth, development, reproduction and cuticle were higher in vector aphids than in non-vector aphids. STAT5B (signal transducer and activator of transcription 5B), which is involved in the JAK-STAT pathway, was upregulated in R. padi exposed to WDV. The cloning and bioinformatic results indicated that the RpSTAT5B sequence contains a 2082 bp ORF encoding 693 amino acids. The protein molecular weight is 79.1 kD and pI is 8.13. Analysis indicated that RpSTAT5B is a non-transmembrane protein and a non-secreted protein. Homology and evolutionary analysis indicated that RpSTAT5B was closely related to R. maidis. CONCLUSIONS: Unigene expression analysis showed that the total number of differentially expressed genes (DEGs) in the vector aphids was higher than that in the non-vector aphids. Functional enrichment analysis showed that the DEGs related to immunity, growth and reproduction in vector aphids were higher than those in non-vector aphids, and the differentially expressed genes related to immune were up-regulated. This study provides a basis for the evaluation of the response mechanisms of vector/non-vector insects to plant viruses. |
format | Online Article Text |
id | pubmed-7493910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74939102020-09-23 Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants Li, Dandan Zhang, Chi Tong, Zeqian Su, Dan Zhang, Gaisheng Zhang, Shize Zhao, Huiyan Hu, Zuqing BMC Genomics Research Article BACKGROUND: Plant viruses maintain intricate interactions with their vector and non-vector insects and can impact the fitness of insects. However, the details of their molecular and cellular mechanisms have not been studied well. We compared the transcriptome-level responses in vector and non-vector aphids (Schizaphis graminum and Rhopalosiphum padi, respectively) after feeding on wheat plants with viral infections (Barley Yellow Dwarf Virus (BYDV) and Wheat dwarf virus (WDV), respectively). We conducted differentially expressed gene (DEG) annotation analyses and observed DEGs related to immune pathway, growth, development, and reproduction. And we conducted cloning and bioinformatic analyses of the key DEG involved in immune. RESULTS: For all differentially expressed gene analyses, the numbers of DEGs related to immune, growth, development, reproduction and cuticle were higher in vector aphids than in non-vector aphids. STAT5B (signal transducer and activator of transcription 5B), which is involved in the JAK-STAT pathway, was upregulated in R. padi exposed to WDV. The cloning and bioinformatic results indicated that the RpSTAT5B sequence contains a 2082 bp ORF encoding 693 amino acids. The protein molecular weight is 79.1 kD and pI is 8.13. Analysis indicated that RpSTAT5B is a non-transmembrane protein and a non-secreted protein. Homology and evolutionary analysis indicated that RpSTAT5B was closely related to R. maidis. CONCLUSIONS: Unigene expression analysis showed that the total number of differentially expressed genes (DEGs) in the vector aphids was higher than that in the non-vector aphids. Functional enrichment analysis showed that the DEGs related to immunity, growth and reproduction in vector aphids were higher than those in non-vector aphids, and the differentially expressed genes related to immune were up-regulated. This study provides a basis for the evaluation of the response mechanisms of vector/non-vector insects to plant viruses. BioMed Central 2020-09-15 /pmc/articles/PMC7493910/ /pubmed/32933469 http://dx.doi.org/10.1186/s12864-020-07057-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Li, Dandan Zhang, Chi Tong, Zeqian Su, Dan Zhang, Gaisheng Zhang, Shize Zhao, Huiyan Hu, Zuqing Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
title | Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
title_full | Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
title_fullStr | Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
title_full_unstemmed | Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
title_short | Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
title_sort | transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493910/ https://www.ncbi.nlm.nih.gov/pubmed/32933469 http://dx.doi.org/10.1186/s12864-020-07057-0 |
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