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Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

BACKGROUND: To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, the establishment of a PEG-mediated banana protoplast transformation system makes it possible to build an efficient DNA-free method for a site-directed mutagenesis system. RESULTS: P...

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Detalles Bibliográficos
Autores principales:	Wu, Shaoping, Zhu, Haocheng, Liu, Jinxing, Yang, Qiaosong, Shao, Xiuhong, Bi, Fangcheng, Hu, Chunhua, Huo, Heqiang, Chen, Kunling, Yi, Ganjun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493974/ https://www.ncbi.nlm.nih.gov/pubmed/32933485 http://dx.doi.org/10.1186/s12870-020-02609-8

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