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Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults

BACKGROUND: Older adults exposed to periods of inactivity during hospitalization, illness, or injury lose muscle mass and strength. This, in turn, predisposes poor recovery of physical function upon reambulation and represents a significant health risk for older adults. Bed rest (BR) results in alte...

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Autores principales: Standley, Robert A, Distefano, Giovanna, Trevino, Michelle B, Chen, Emily, Narain, Niven R, Greenwood, Bennett, Kondakci, Gramoz, Tolstikov, Vladimir V, Kiebish, Michael A, Yu, Gongxin, Qi, Feng, Kelly, Daniel P, Vega, Rick B, Coen, Paul M, Goodpaster, Bret H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494044/
https://www.ncbi.nlm.nih.gov/pubmed/31907525
http://dx.doi.org/10.1093/gerona/glaa001
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author Standley, Robert A
Distefano, Giovanna
Trevino, Michelle B
Chen, Emily
Narain, Niven R
Greenwood, Bennett
Kondakci, Gramoz
Tolstikov, Vladimir V
Kiebish, Michael A
Yu, Gongxin
Qi, Feng
Kelly, Daniel P
Vega, Rick B
Coen, Paul M
Goodpaster, Bret H
author_facet Standley, Robert A
Distefano, Giovanna
Trevino, Michelle B
Chen, Emily
Narain, Niven R
Greenwood, Bennett
Kondakci, Gramoz
Tolstikov, Vladimir V
Kiebish, Michael A
Yu, Gongxin
Qi, Feng
Kelly, Daniel P
Vega, Rick B
Coen, Paul M
Goodpaster, Bret H
author_sort Standley, Robert A
collection PubMed
description BACKGROUND: Older adults exposed to periods of inactivity during hospitalization, illness, or injury lose muscle mass and strength. This, in turn, predisposes poor recovery of physical function upon reambulation and represents a significant health risk for older adults. Bed rest (BR) results in altered skeletal muscle fuel metabolism and loss of oxidative capacity that have recently been linked to the muscle atrophy program. Our primary objective was to explore the effects of BR on mitochondrial energetics in muscle from older adults. A secondary objective was to examine the effect of β-hydroxy-β-methylbuturate (HMB) supplementation on mitochondrial energetics. METHODS: We studied 20 older adults before and after a 10-day BR intervention, who consumed a complete oral nutritional supplement (ONS) with HMB (3.0 g/d HMB, n = 11) or without HMB (CON, n = 9). Percutaneous biopsies of the vastus lateralis were obtained to determine mitochondrial respiration and H(2)O(2) emission in permeabilized muscle fibers along with markers of content. RNA sequencing and lipidomics analyses were also conducted. RESULTS: We found a significant up-regulation of collagen synthesis and down-regulation of ribosome, oxidative metabolism and mitochondrial gene transcripts following BR in the CON group. Alterations to these gene transcripts were significantly blunted in the HMB group. Mitochondrial respiration and markers of content were both reduced and H(2)O(2) emission was elevated in both groups following BR. CONCLUSIONS: In summary, 10 days of BR in older adults causes a significant deterioration in mitochondrial energetics, while transcriptomic profiling revealed that some of these negative effects may be attenuated by an ONS containing HMB.
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spelling pubmed-74940442020-09-21 Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults Standley, Robert A Distefano, Giovanna Trevino, Michelle B Chen, Emily Narain, Niven R Greenwood, Bennett Kondakci, Gramoz Tolstikov, Vladimir V Kiebish, Michael A Yu, Gongxin Qi, Feng Kelly, Daniel P Vega, Rick B Coen, Paul M Goodpaster, Bret H J Gerontol A Biol Sci Med Sci THE JOURNAL OF GERONTOLOGY: Medical Sciences BACKGROUND: Older adults exposed to periods of inactivity during hospitalization, illness, or injury lose muscle mass and strength. This, in turn, predisposes poor recovery of physical function upon reambulation and represents a significant health risk for older adults. Bed rest (BR) results in altered skeletal muscle fuel metabolism and loss of oxidative capacity that have recently been linked to the muscle atrophy program. Our primary objective was to explore the effects of BR on mitochondrial energetics in muscle from older adults. A secondary objective was to examine the effect of β-hydroxy-β-methylbuturate (HMB) supplementation on mitochondrial energetics. METHODS: We studied 20 older adults before and after a 10-day BR intervention, who consumed a complete oral nutritional supplement (ONS) with HMB (3.0 g/d HMB, n = 11) or without HMB (CON, n = 9). Percutaneous biopsies of the vastus lateralis were obtained to determine mitochondrial respiration and H(2)O(2) emission in permeabilized muscle fibers along with markers of content. RNA sequencing and lipidomics analyses were also conducted. RESULTS: We found a significant up-regulation of collagen synthesis and down-regulation of ribosome, oxidative metabolism and mitochondrial gene transcripts following BR in the CON group. Alterations to these gene transcripts were significantly blunted in the HMB group. Mitochondrial respiration and markers of content were both reduced and H(2)O(2) emission was elevated in both groups following BR. CONCLUSIONS: In summary, 10 days of BR in older adults causes a significant deterioration in mitochondrial energetics, while transcriptomic profiling revealed that some of these negative effects may be attenuated by an ONS containing HMB. Oxford University Press 2020-01-07 /pmc/articles/PMC7494044/ /pubmed/31907525 http://dx.doi.org/10.1093/gerona/glaa001 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle THE JOURNAL OF GERONTOLOGY: Medical Sciences
Standley, Robert A
Distefano, Giovanna
Trevino, Michelle B
Chen, Emily
Narain, Niven R
Greenwood, Bennett
Kondakci, Gramoz
Tolstikov, Vladimir V
Kiebish, Michael A
Yu, Gongxin
Qi, Feng
Kelly, Daniel P
Vega, Rick B
Coen, Paul M
Goodpaster, Bret H
Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults
title Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults
title_full Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults
title_fullStr Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults
title_full_unstemmed Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults
title_short Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults
title_sort skeletal muscle energetics and mitochondrial function are impaired following 10 days of bed rest in older adults
topic THE JOURNAL OF GERONTOLOGY: Medical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494044/
https://www.ncbi.nlm.nih.gov/pubmed/31907525
http://dx.doi.org/10.1093/gerona/glaa001
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