Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage

In the context of an unprecedented shortage of nasopharyngeal swabs (NPS) or sample transport media during the coronavirus disease 2019 (COVID-19) crisis, alternative methods for sample collection are needed. To address this need, we validated a cell culture medium as a viral transport medium, and c...

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Autores principales: Calame, Adrien, Mazza, Léna, Renzoni, Adriana, Kaiser, Laurent, Schibler, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494432/
https://www.ncbi.nlm.nih.gov/pubmed/32939576
http://dx.doi.org/10.1007/s10096-020-04039-8
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author Calame, Adrien
Mazza, Léna
Renzoni, Adriana
Kaiser, Laurent
Schibler, Manuel
author_facet Calame, Adrien
Mazza, Léna
Renzoni, Adriana
Kaiser, Laurent
Schibler, Manuel
author_sort Calame, Adrien
collection PubMed
description In the context of an unprecedented shortage of nasopharyngeal swabs (NPS) or sample transport media during the coronavirus disease 2019 (COVID-19) crisis, alternative methods for sample collection are needed. To address this need, we validated a cell culture medium as a viral transport medium, and compared the analytical sensitivity of SARS-CoV-2 RT-PCR in nasal wash (NW), oropharyngeal swab (OPS), and NPS specimens. Both the clinical and analytical sensitivity were comparable in these three sample types. OPS and NW specimens may therefore represent suitable alternatives to NPS for SARS-CoV-2 detection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10096-020-04039-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-74944322020-09-17 Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage Calame, Adrien Mazza, Léna Renzoni, Adriana Kaiser, Laurent Schibler, Manuel Eur J Clin Microbiol Infect Dis Brief Report In the context of an unprecedented shortage of nasopharyngeal swabs (NPS) or sample transport media during the coronavirus disease 2019 (COVID-19) crisis, alternative methods for sample collection are needed. To address this need, we validated a cell culture medium as a viral transport medium, and compared the analytical sensitivity of SARS-CoV-2 RT-PCR in nasal wash (NW), oropharyngeal swab (OPS), and NPS specimens. Both the clinical and analytical sensitivity were comparable in these three sample types. OPS and NW specimens may therefore represent suitable alternatives to NPS for SARS-CoV-2 detection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10096-020-04039-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-09-17 2021 /pmc/articles/PMC7494432/ /pubmed/32939576 http://dx.doi.org/10.1007/s10096-020-04039-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Report
Calame, Adrien
Mazza, Léna
Renzoni, Adriana
Kaiser, Laurent
Schibler, Manuel
Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage
title Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage
title_full Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage
title_fullStr Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage
title_full_unstemmed Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage
title_short Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage
title_sort sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for sars-cov-2 detection in the setting of sampling device shortage
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494432/
https://www.ncbi.nlm.nih.gov/pubmed/32939576
http://dx.doi.org/10.1007/s10096-020-04039-8
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