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Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients
We have examined the Acylated Ghrelin (AG)/Gi pathway in different human osteoblastic cell lines. We have found that: 1) AG induces differentiation/mineralization only in mature osteoblasts; 2) the expression of GHS-R1a increases up to the mature cell stage, 3) the action is mediated via the GHS-R/G...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494670/ https://www.ncbi.nlm.nih.gov/pubmed/32984555 http://dx.doi.org/10.1016/j.bbrep.2020.100782 |
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author | Barre, Ronan Beton, Nicolas Batut, Aurélie Accabled, Frank Sales de Gauzy, Jerome Auriol, Françoise Eddiry, Sanaa Tauber, Maithe Laurencin, Sara Salles, Jean Pierre Gennero, Isabelle |
author_facet | Barre, Ronan Beton, Nicolas Batut, Aurélie Accabled, Frank Sales de Gauzy, Jerome Auriol, Françoise Eddiry, Sanaa Tauber, Maithe Laurencin, Sara Salles, Jean Pierre Gennero, Isabelle |
author_sort | Barre, Ronan |
collection | PubMed |
description | We have examined the Acylated Ghrelin (AG)/Gi pathway in different human osteoblastic cell lines. We have found that: 1) AG induces differentiation/mineralization only in mature osteoblasts; 2) the expression of GHS-R1a increases up to the mature cell stage, 3) the action is mediated via the GHS-R/Gi/cAMP pathway only in mature osteoblasts, and 4) osteoblastic cells from adolescent idiopathic scoliosis (AIS) are resistant to the AG/Gi/cAMP pathway. Altogether, these results suggested that AG uses the GHS-R1a/Gi/cAMP pathway to induce differentiation in mature osteoblasts only. This pathway is impaired in AIS osteoblasts. Understanding AG-specific pathways involved in normal and pathological osteoblasts may be useful for developing new treatments for pathologies such as AIS or osteoporosis. |
format | Online Article Text |
id | pubmed-7494670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-74946702020-09-24 Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients Barre, Ronan Beton, Nicolas Batut, Aurélie Accabled, Frank Sales de Gauzy, Jerome Auriol, Françoise Eddiry, Sanaa Tauber, Maithe Laurencin, Sara Salles, Jean Pierre Gennero, Isabelle Biochem Biophys Rep Research Article We have examined the Acylated Ghrelin (AG)/Gi pathway in different human osteoblastic cell lines. We have found that: 1) AG induces differentiation/mineralization only in mature osteoblasts; 2) the expression of GHS-R1a increases up to the mature cell stage, 3) the action is mediated via the GHS-R/Gi/cAMP pathway only in mature osteoblasts, and 4) osteoblastic cells from adolescent idiopathic scoliosis (AIS) are resistant to the AG/Gi/cAMP pathway. Altogether, these results suggested that AG uses the GHS-R1a/Gi/cAMP pathway to induce differentiation in mature osteoblasts only. This pathway is impaired in AIS osteoblasts. Understanding AG-specific pathways involved in normal and pathological osteoblasts may be useful for developing new treatments for pathologies such as AIS or osteoporosis. Elsevier 2020-09-11 /pmc/articles/PMC7494670/ /pubmed/32984555 http://dx.doi.org/10.1016/j.bbrep.2020.100782 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Barre, Ronan Beton, Nicolas Batut, Aurélie Accabled, Frank Sales de Gauzy, Jerome Auriol, Françoise Eddiry, Sanaa Tauber, Maithe Laurencin, Sara Salles, Jean Pierre Gennero, Isabelle Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients |
title | Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients |
title_full | Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients |
title_fullStr | Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients |
title_full_unstemmed | Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients |
title_short | Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients |
title_sort | ghrelin uses the ghs-r1a/gi/camp pathway and induces differentiation only in mature osteoblasts. this ghrelin pathway is impaired in ais patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494670/ https://www.ncbi.nlm.nih.gov/pubmed/32984555 http://dx.doi.org/10.1016/j.bbrep.2020.100782 |
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