Cargando…
Isolation of mesenchymal stem cells from Pap smear samples
OBJECTIVE: Exploiting their ability to differentiate into mesenchymal lineages like cartilage, bone, fat, and muscle, and to elicit paracrine effects, mesenchymal stem cells (MSCs) are widely used in clinical settings to treat tissue injuries and autoimmune disorders. One of accessible sources of MS...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Obstetrics and Gynecology
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494764/ https://www.ncbi.nlm.nih.gov/pubmed/32698560 http://dx.doi.org/10.5468/ogs.20073 |
_version_ | 1783582793006055424 |
---|---|
author | Park, Junghyun Son, Daryeon Hong, Wonjun Jang, Jihoon Cho, Geum Joon Song, Gwonhwa Kim, In Yong You, Seungkwon |
author_facet | Park, Junghyun Son, Daryeon Hong, Wonjun Jang, Jihoon Cho, Geum Joon Song, Gwonhwa Kim, In Yong You, Seungkwon |
author_sort | Park, Junghyun |
collection | PubMed |
description | OBJECTIVE: Exploiting their ability to differentiate into mesenchymal lineages like cartilage, bone, fat, and muscle, and to elicit paracrine effects, mesenchymal stem cells (MSCs) are widely used in clinical settings to treat tissue injuries and autoimmune disorders. One of accessible sources of MSC is the samples used for Papanicolaou (Pap) test, which is a cervical screening method for detecting potentially pre-cancerous and cancerous alterations in the cervical cells and to diagnose genetic abnormalities in fetuses. This study aimed to identify and isolate the stem cells from Pap smear samples collected from pregnant women, and to trace the origin of these cells to maternal or fetal tissue, and characterize their stem cell properties. METHODS: To investigate the possibility and efficiency of establishing MSC lines from the Pap smear samples, we were able to establish 6 cell lines from Pap smear samples from 60 pregnant women at different stages of gestation. RESULTS: The 3 cell lines randomly selected among the 6 established in this study, displayed high proliferation rates, several characteristics of MSCs, and the capacity to differentiate into adipocytes, osteocytes, and chondrocytes. Our study identified that the stem cell lines obtainable from Pap smear sampling were uterine cervical stromal cells (UCSCs) and had 10% efficiency of establishment. CONCLUSION: Despite their low efficiency of establishment, human UCSCs from Pap smear samples can become a simple, safe, low-cost, and donor-specific source of MSCs for stem cell therapy and regenerative medicine. |
format | Online Article Text |
id | pubmed-7494764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society of Obstetrics and Gynecology |
record_format | MEDLINE/PubMed |
spelling | pubmed-74947642020-09-24 Isolation of mesenchymal stem cells from Pap smear samples Park, Junghyun Son, Daryeon Hong, Wonjun Jang, Jihoon Cho, Geum Joon Song, Gwonhwa Kim, In Yong You, Seungkwon Obstet Gynecol Sci Original Article OBJECTIVE: Exploiting their ability to differentiate into mesenchymal lineages like cartilage, bone, fat, and muscle, and to elicit paracrine effects, mesenchymal stem cells (MSCs) are widely used in clinical settings to treat tissue injuries and autoimmune disorders. One of accessible sources of MSC is the samples used for Papanicolaou (Pap) test, which is a cervical screening method for detecting potentially pre-cancerous and cancerous alterations in the cervical cells and to diagnose genetic abnormalities in fetuses. This study aimed to identify and isolate the stem cells from Pap smear samples collected from pregnant women, and to trace the origin of these cells to maternal or fetal tissue, and characterize their stem cell properties. METHODS: To investigate the possibility and efficiency of establishing MSC lines from the Pap smear samples, we were able to establish 6 cell lines from Pap smear samples from 60 pregnant women at different stages of gestation. RESULTS: The 3 cell lines randomly selected among the 6 established in this study, displayed high proliferation rates, several characteristics of MSCs, and the capacity to differentiate into adipocytes, osteocytes, and chondrocytes. Our study identified that the stem cell lines obtainable from Pap smear sampling were uterine cervical stromal cells (UCSCs) and had 10% efficiency of establishment. CONCLUSION: Despite their low efficiency of establishment, human UCSCs from Pap smear samples can become a simple, safe, low-cost, and donor-specific source of MSCs for stem cell therapy and regenerative medicine. Korean Society of Obstetrics and Gynecology 2020-09 2020-07-23 /pmc/articles/PMC7494764/ /pubmed/32698560 http://dx.doi.org/10.5468/ogs.20073 Text en Copyright © 2020 Korean Society of Obstetrics and Gynecology Articles published in Obstet Gynecol Sci are open-access, distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Junghyun Son, Daryeon Hong, Wonjun Jang, Jihoon Cho, Geum Joon Song, Gwonhwa Kim, In Yong You, Seungkwon Isolation of mesenchymal stem cells from Pap smear samples |
title | Isolation of mesenchymal stem cells from Pap smear samples |
title_full | Isolation of mesenchymal stem cells from Pap smear samples |
title_fullStr | Isolation of mesenchymal stem cells from Pap smear samples |
title_full_unstemmed | Isolation of mesenchymal stem cells from Pap smear samples |
title_short | Isolation of mesenchymal stem cells from Pap smear samples |
title_sort | isolation of mesenchymal stem cells from pap smear samples |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494764/ https://www.ncbi.nlm.nih.gov/pubmed/32698560 http://dx.doi.org/10.5468/ogs.20073 |
work_keys_str_mv | AT parkjunghyun isolationofmesenchymalstemcellsfrompapsmearsamples AT sondaryeon isolationofmesenchymalstemcellsfrompapsmearsamples AT hongwonjun isolationofmesenchymalstemcellsfrompapsmearsamples AT jangjihoon isolationofmesenchymalstemcellsfrompapsmearsamples AT chogeumjoon isolationofmesenchymalstemcellsfrompapsmearsamples AT songgwonhwa isolationofmesenchymalstemcellsfrompapsmearsamples AT kiminyong isolationofmesenchymalstemcellsfrompapsmearsamples AT youseungkwon isolationofmesenchymalstemcellsfrompapsmearsamples |